创面负压治疗对糖尿病足创面肉芽组织胶原蛋白沉积的影响
发布时间：2018-08-01 08:23
【摘要】：目的探讨创面负压治疗(NPWT)对糖尿病足(DF)肉芽组织胶原蛋白沉积的影响。方法选取2014年1月至2016年6月在解放军白求恩国际和平医院内分泌科住院的DF患者40例,随机分为NPWT组和对照组(Con),每组各20例。NPWT组给予NPWT治疗,Con组给予常规换药治疗。两组分别于治疗前及治疗第14天取创面肉芽组织,行免疫组织化学染色,Western blot和RT-PCR分析Ⅰ、Ⅲ型胶原蛋白表达情况。结果免疫组织化学分析显示,两组治疗后Ⅰ型、Ⅲ型胶原蛋白平均面密度值较治疗前升高,NPWT组升高的程度高于Con组[(0.12±0.03)vs(0.34±0.04),(0.06±0.03)vs(0.19±0.03),P0.01]。Western blot分析显示,两组治疗后Ⅰ型、Ⅲ型胶原蛋白相对蛋白表达值较治疗前均升高,但NPWT组升高的程度高于Con组[(0.10±.042)vs(0.25±0.08),(0.11±0.03)vs(0.19±0.07),P0.01]。RT-PCR分析显示,两组治疗后Ⅰ型、Ⅲ型胶原蛋白mRNA相对表达值较治疗前均升高,但NPWT组升高程度高于Con组[(0.94±0.29)vs(3.24±0.54),(1.13±0.45)vs(3.13±1.20),P0.01]。结论 NPWT可促明显进创面肉芽组织Ⅰ、Ⅲ型胶原蛋白表达上调,从而促进创面的愈合。
[Abstract]:Objective to investigate the effect of wound negative pressure (NPWT) on the collagen deposition in the granulation tissue of diabetic foot (DF). Methods 40 patients with DF hospitalized in Department of Endocrinology, Heping Hospital, liberation army, from January 2014 to June 2016 were randomly divided into NPWT group and control group (Con). Each group was given NPWT treatment in each group of.NPWT, and Con group was given a regular basis. The two groups were treated with the wound granulation tissue before and fourteenth days of treatment. The immunohistochemical staining was performed in the two groups. The expression of type I and type III collagen was analyzed by Western blot and RT-PCR. Results the immunohistochemical analysis showed that after two groups of treatment, the surface density of type I and type III collagen egg was higher than that before the treatment, and the NPWT group increased. The degree was higher than that of Con Group [(0.12 + 0.03) vs (0.34 + 0.04), (0.06 + 0.03) vs (0.19 + 0.03), P0.01].Western blot analysis showed that the relative protein expression value of type I and type III collagen in the two group was higher than that before treatment, but the level of NPWT group was higher than that of Con Group [0.10 +.042) vs (0.25 + 0.08) and P0.01].RT-PCR analysis, P0.01].RT-PCR analysis. The results showed that the relative expression value of type I and type III collagen mRNA increased after treatment in the two groups, but the increase in NPWT group was higher than that of group Con [(0.94 + 0.29) vs (3.24 + 0.54), (1.13 + 0.45) vs (3.13 + 1.20). P0.01]. conclusion NPWT can promote the wound granulation tissue to be obviously up-regulated, thus promoting the healing of the wound.
【作者单位】： 解放军白求恩国际和平医院内分泌科;河北医科大学研究生学院;武警山东总队医院内分泌科;解放军总医院内分泌科;
【基金】：河北省科技支撑计划项目(12277725)
【分类号】：R587.2
本文编号：2156956
[Abstract]:Objective to investigate the effect of wound negative pressure (NPWT) on the collagen deposition in the granulation tissue of diabetic foot (DF). Methods 40 patients with DF hospitalized in Department of Endocrinology, Heping Hospital, liberation army, from January 2014 to June 2016 were randomly divided into NPWT group and control group (Con). Each group was given NPWT treatment in each group of.NPWT, and Con group was given a regular basis. The two groups were treated with the wound granulation tissue before and fourteenth days of treatment. The immunohistochemical staining was performed in the two groups. The expression of type I and type III collagen was analyzed by Western blot and RT-PCR. Results the immunohistochemical analysis showed that after two groups of treatment, the surface density of type I and type III collagen egg was higher than that before the treatment, and the NPWT group increased. The degree was higher than that of Con Group [(0.12 + 0.03) vs (0.34 + 0.04), (0.06 + 0.03) vs (0.19 + 0.03), P0.01].Western blot analysis showed that the relative protein expression value of type I and type III collagen in the two group was higher than that before treatment, but the level of NPWT group was higher than that of Con Group [0.10 +.042) vs (0.25 + 0.08) and P0.01].RT-PCR analysis, P0.01].RT-PCR analysis. The results showed that the relative expression value of type I and type III collagen mRNA increased after treatment in the two groups, but the increase in NPWT group was higher than that of group Con [(0.94 + 0.29) vs (3.24 + 0.54), (1.13 + 0.45) vs (3.13 + 1.20). P0.01]. conclusion NPWT can promote the wound granulation tissue to be obviously up-regulated, thus promoting the healing of the wound.
【作者单位】： 解放军白求恩国际和平医院内分泌科;河北医科大学研究生学院;武警山东总队医院内分泌科;解放军总医院内分泌科;
【基金】：河北省科技支撑计划项目(12277725)
【分类号】：R587.2
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