急性有机磷农药中毒患者血清miR-214表达水平及功能

发布时间：2018-08-08 13:11

【摘要】：目的:探讨miR-214在急性有机磷农药中毒(AOPP)患者血清中的表达及其可能的生物学功能。方法:采用实时荧光定量PCR(q RT-PCR)法检测102例AOPP患者和100例健康对照的血清miR-214表达并进行比较,分析miR-214水平与中毒患者胆碱酯酶(Ch E)活力和中毒严重程度评分(PSS)的相关性;利用生物信息学方法预测miR-214可能的靶基因,并对预测靶基因进行功能注释和通路分析。结果:AOPP患者血清miR-214水平明显高于对照组(P0.01),与Ch E活力呈负相关(r=-0.380,P0.01),与PSS评分呈显著正相关(r=0.702,P=0.000);生物信息学分析显示miR-214预测靶基因主要涉及核酸多种组分的合成与代谢、ATP能量的合成与代谢、神经递质转运、蛋白质氨基酸脱磷酸化、调节Ras和GTP介导的信号传导等生物过程,以及多种磷酸酶活性、ATP与GTP酶活性调节、钙离子跨膜转运蛋白活性等分子功能,KEGG信号通路富集于胰岛素信号通路、钙信号通路。结论:miR-214在AOPP患者血清中表达水平升高,并与中毒病情程度相关,可能参与OPs中毒后病理损伤过程。
[Abstract]:Objective: to investigate the expression of miR-214 in serum of patients with acute organophosphorus pesticide poisoning (AOPP) and its possible biological function. Methods: the expression of serum miR-214 was detected by real-time fluorescence quantitative PCR (q RT-PCR in 102 patients with AOPP and 100 healthy controls. The correlation between miR-214 level and cholinesterase (Ch E) activity and severity score (PSS) was analyzed. The possible target genes of miR-214 were predicted by bioinformatics, and the functional annotation and pathway analysis of the predicted target genes were carried out. Results the serum miR-214 level in the patients with miR-214 was significantly higher than that in the control group (P0.01), and negatively correlated with the activity of che (r-0.380, P0.01) and positively with the PSS score (r-0.702, P0. 000). Bioinformatics analysis showed that the predicted target genes of miR-214 were mainly involved in the synthesis and metabolism of multiple components of nucleic acids. Synthesis and Metabolism of ATP Energy, Neurotransmitter transport, protein amino acid dephosphorylation, regulation of signal transduction mediated by Ras and GTP, and regulation of various phosphatase activities such as ATP and GTP activity. Calcium ion transmembrane transporter activity and other molecular functions of KEGG signaling pathway are enriched in insulin signaling pathway and calcium signal pathway. Conclusion the expression level of miR-214 in serum of AOPP patients was increased and correlated with the severity of poisoning. It may be involved in the process of pathological injury after OPs poisoning.
【作者单位】：南华大学附属第二医院急诊科;南华大学附属第二医院神经内科;
【基金】：湖南省教育厅项目(编号:15C1209)
【分类号】：R595.4

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