驻极体外静电场对猪胰岛素结构和介电特性的影响

发布时间：2018-08-09 08:16

【摘要】：为研究驻极体外静电场对猪胰岛素结构和介电特性的影响,从而为进一步研究驻极体提高胰岛素的降糖能力提供理论依据,以常温电晕充电下制备得到的500 V、1 000 V和1 500 V聚丙烯驻极体为电场源作用于胰岛素,测量药物的d33系数及核磁共振和非变性凝胶电泳谱。研究结果表明:1)不同表面电位聚丙烯驻极体产生的外静电场能使胰岛素溶液和胰岛素贴剂两侧形成相对稳定的静电场,电场强度与驻极体的等效表面电位成正比;2)驻极体外静电场对胰岛素的一级结构和二级结构没有明显影响;3)经500 V、1 000 V和1 500 V驻极体作用的胰岛素贴剂的d33值分别较胰岛素贴剂提高15.7倍、21.3倍和32.0倍;4)核磁共振谱表明驻极体作用后的胰岛素结构中氢键网络发生了改变。因此,驻极体产生的静电场在一定程度上引起了胰岛素分子的极化,改变了胰岛素的带电性,有利于胰岛素单体的形成,并能够提高胰岛素的降糖能力。
[Abstract]:In order to study the effect of electret external electrostatic field on the structure and dielectric properties of porcine insulin, and to provide a theoretical basis for further study on the enhancement of insulin hypoglycemic ability by electret. 500 V / 1 000 V and 1 500 V polypropylene electret prepared under normal temperature corona charge acted on insulin. The d33 coefficient of the drug, NMR and non-denaturing gel electrophoresis spectra were measured. The results show that the external electrostatic field produced by polypropylene electret with different surface potentials can form a relatively stable electrostatic field on both sides of insulin solution and insulin patch. The electric field intensity is proportional to the equivalent surface potential of the electret. 2) the electrostatic field outside the electret has no significant effect on the primary and secondary structure of insulin. Compared with insulin patch, it was 15.7 times higher by 21.3 times and 32.0 times than that of insulin patch, respectively. NMR spectra showed that the hydrogen bond network in insulin structure changed after electret action. Therefore, the electrostatic field produced by the electret to a certain extent caused the polarization of insulin molecules, changed the charged character of insulin, was conducive to the formation of insulin monomer, and could improve the ability of insulin to reduce glucose.
【作者单位】：第二军医大学药学院无机教研室;第二军医大学基础部数理教研室;
【基金】：国家自然科学基金(51477175)~~
【分类号】：R587.1

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