高通量测序技术研究糖尿病大鼠与正常大鼠粪便菌群的结构差异

发布时间：2018-08-14 10:17

【摘要】：为研究糖尿病大鼠和正常大鼠粪便菌群的结构差异,选取SD雄性大鼠,随机分为正常组和糖尿病组,于第4周末,对大鼠粪便菌群16S r RNA的V1~V3区进行扩增,Miseq高通量测序平台测定其基因序列,与silva数据库中微生物基因序列进行比对、分类。研究发现:糖尿病大鼠体重较正常组显著下降;糖尿病大鼠粪便菌群多样性高于正常大鼠;糖尿病大鼠粪便菌群中肠杆菌目,假单孢杆菌目等机会致病菌增多;同时克里斯滕森菌科,明串珠菌科,黄杆菌科,Clostridium sensu stricto 1,Gastranaerophilales_norank,Ruminococcaceae_incertae_sedis等在糖尿病组粪便中大量存在。结果表明:糖尿病大鼠肠道菌群结构与正常大鼠差异显著,存在肠道菌群紊乱的状况,提示临床上可通过调节饮食结构来改善肠道菌群,达到防治2型糖尿病的目的。
[Abstract]:In order to study the difference of fecal flora between diabetic rats and normal rats, SD male rats were randomly divided into normal group and diabetic group at the end of the 4th week. The V1~V3 region of 16s r RNA of rat faecal flora was amplified by high throughput sequencing platform. The gene sequence was compared with that of microorganism in silva database and classified. The results showed that the body weight of diabetic rats was significantly lower than that of normal rats, the fecal microflora diversity of diabetic rats was higher than that of normal rats, the fecal flora of diabetic rats was increased, and the number of opportunistic pathogens such as Enterobacteriae, pseudomonas and so on were increased in diabetic rats. At the same time, Clostridium sensu stricto 1 and gastranaerophilia cells were found in the feces of diabetes mellitus group, such as Christensen, Streptomycaceae, Serulidae, Serulidae, Serulidae, and Clostridium sensu stricto 1 / gastranaerophilia sp. Ruminococcaceaeincertaesedis, and so on. The results showed that the intestinal flora structure of diabetic rats was significantly different from that of normal rats, and the intestinal microflora disorder existed, which suggested that the intestinal flora could be improved by adjusting dietary structure in order to prevent and treat type 2 diabetes mellitus.
【作者单位】：天津科技大学食品工程与生物技术学院;
【基金】：国家自然科学基金项目(31471701) 科技部中欧合作资金项目(SQ2013ZOA100001)
【分类号】：R587.1

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