难治性系统性红斑狼疮与巨细胞病毒感染的关系

发布时间：2018-08-14 11:38

【摘要】：目的探讨难治性系统性红斑狼疮(refractory systemic lupus erythematosus,RSLE)与巨细胞病毒(CMV)感染的关系。方法收集本院门诊和住院治疗的45例RSLE患者,根据CMV-DNA检测结果,分为CMV阳性RSLE组和CMV阴性RSLE组,另有46位同期来本院查体的健康体检者设为正常对照组。采用实时定量PCR检测RSLE患者和正常对照组外周血单个核细胞或者尿液上皮细胞中的CMV-DNA;四色流式细胞术检测RSLE患者和正常对照组外周血CD3~+,CD4~+,CD8~+T淋巴细胞。结果RSLE患者CMV阳性率(38/45,84.4%)显著高于正常对照组(3/46,6.5%,P0.01);CMV阳性RSLE患者临床特征与CMV阴性RSLE相比,差异均有统计学意义(均P0.05);CMV-DNA载量与CMV阳性RSLE患者疾病活动性指标呈正相关(均P0.05),与补体C_3呈负相关(P0.05);CMV阳性RSLE组CD3~+,CD4~+,CD8~+T淋巴细胞计数及总淋巴细胞计数显著高于CMV阴性RSLE组和正常对照组(均P0.05),而CD4~+/CD8~+显著低于CMV阴性RSLE和正常对照组(均P0.05)。结论RSLE患者应行巨细胞病毒检查,针对性治疗,提高疗效。
[Abstract]:Objective to investigate the relationship between refractory systemic lupus erythematosus (refractory systemic lupus erythematosus) and cytomegalovirus (CMV) infection. Methods 45 patients with RSLE were divided into CMV positive RSLE group and CMV negative RSLE group according to the results of CMV-DNA test. CMV-DNA in peripheral blood mononuclear cells or urine epithelial cells in patients with RSLE and normal controls were detected by real-time quantitative PCR, and CD3 ~ + CD4 ~ + CD8 ~ T lymphocytes in peripheral blood of RSLE patients and normal controls were detected by four-color flow cytometry. Results the positive rate of CMV in RSLE patients (38 / 45, 84.4%) was significantly higher than that in normal controls (3 / 46, 6.5%, P0.01). The clinical characteristics of CMV-positive RSLE patients were compared with those of CMV negative RSLE. The difference was statistically significant (P0.05). There was a positive correlation between CMV-DNA load and disease activity in patients with CMV positive RSLE (P0.05), and a negative correlation with complement C3 (P0.05). The number of CD3 ~ + CD4 ~ + CD8 ~ T lymphocytes and total lymphocyte counts in RSLE group were significantly higher than those in RSLE group. CMV negative RSLE group and normal control group (P0.05), while CD4 ~ / CD8 ~ significantly lower than CMV negative RSLE and normal control group (P0.05). Conclusion cytomegalovirus examination should be performed in patients with RSLE.
【作者单位】：江西省萍乡市人民医院;
【基金】：江西省卫计委课题(20157142) 江西省萍乡市科技局计划项目(2013NS017)
【分类号】：R593.241

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