宏基因组研究高脂饮食诱导小鼠的肥胖易感性与肠道菌群的关系

发布时间：2018-08-16 18:04

【摘要】：目的考察高脂饮食喂养后小鼠肠道菌群在组成及功能上与肥胖易感的相关性。方法采用高脂饲料喂养C57BL/6J小鼠构建高脂饮食诱导肥胖组(high-fat-diet-induced-obesity,HFDIO)和高脂饮食诱导肥胖抵抗组(high-fat-diet-induced-obesity-resistant,HFDIOR)模型,正常饲料组为对照组,搜集并提取3组小鼠粪便样本细菌基因组,DNA质检后挑取高质量样本(每组6个),采用Illumina公司的Hiseq2000进行细菌宏基因组测序及相关生物信息学与统计学分析。结果较之对照组,肥胖组和肥胖抵抗组小鼠肠道厚壁菌门、变形菌门、脱铁杆菌门含量显著升高,拟杆菌门显著下降(P0.05)。肥胖组和肥胖抵抗组小鼠肠道的特征菌种组成显著不同,且毛螺菌科中的Lachnospiraceae bacterium 10_1与Lachnospiraceae bacterium 28_4分别为肥胖及肥胖抵抗组小鼠的关键菌种;肥胖与肥胖抵抗小鼠肠道菌群的功能及代谢显著差异则主要集中在碳水化合物代谢、核酸代谢与锚定、膜转运活性及转移酶活性等方面。结论不同个体对于饮食诱导的肥胖的易感性差别可能与肠道菌群组成及功能变化相关,这有助于正确评价肠道菌群在肥胖发生中的作用。
[Abstract]:Objective to investigate the relationship between the composition and function of intestinal microflora and the susceptibility to obesity in mice fed with high fat diet. Methods High-fat-diet-induced obesity group (HFDIO) and high-fat-diet-induced obesity-resistance-resistant (HFDIOR) model were established in C57BL/6J mice fed with high-fat diet, and the normal diet group was used as control group. Three groups of fecal samples of mice were collected and extracted. The samples of high quality samples (6 in each group) were collected and collected. The microgenome sequencing and bioinformatics and statistical analysis were carried out by Hiseq2000 of Illumina Company. Results compared with the control group, the contents of intestinal phylum, Proteus, ferribacter in obese group and obesity resistance group were significantly higher than those in control group, but Bacteroides significantly decreased (P0.05). The composition of characteristic bacteria in the intestinal tract of obese and obese resistant mice was significantly different, and Lachnospiraceae bacterium 10S1 and Lachnospiraceae bacterium 284 were the key strains of obese and obese resistant mice, respectively. The significant differences in intestinal microflora function and metabolism between obese and obese resistant mice were mainly concentrated in carbohydrate metabolism, nucleic acid metabolism and anchoring, membrane transport activity and transferase activity. Conclusion the susceptibility of different individuals to diet-induced obesity may be related to the composition and function of intestinal flora, which is helpful to evaluate the role of intestinal flora in obesity.
【作者单位】：第三军医大学基础医学部微生物学教研室重庆市微生物工程实验室;
【分类号】：R589.2

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