自噬相关基因mTOR,Becline-1,LC3和p62在系统性红斑狼疮患者外周血单个核细胞中的表达及意义
发布时间：2018-08-17 10:13
【摘要】：目的通过检测系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMCs)中自噬相关基因(Atgs)mTOR,Becline-1,LC3和p62的表达情况,分析其与SLE疾病活动度(SLEDAI)及免疫学指标的相关性,初步探讨mTOR,Becline-1,LC3和p62在SLE发生发展中的作用。方法1.81例2015.10-2016.05就诊于郑州大学第一附属医院风湿免疫科的SLE患者为实验组,采集所有实验对象的临床、人口及实验室资料。同时期来自郑州大学第一附属医院体检科的健康志愿者86例为健康对照。清晨采集所有研究对象血标本。2.RT-PCR检测81例SLE患者及86例健康对照PBMCs中Atgs mTOR,Becline-1,LC3和p62的mRNA水平。分析SLE组和健康对照组间Atgs表达的差异及其与SLEDAI及免疫学指标的相关性。3.统计学分析采用统计分析软件SPSS21.0,均数±标准差((?)±S)与百分比(%)分别表示定量资料与定性资料。两样本间定量资料的比较采用t检验,多组定量资料的比较采用单因素方差分析或非参数检验。定性资料比较采用χ2检验。Person,s或Spearman秩相关分析用来评价Atgs mRNA水平与SLEDAI及免疫学指标的相关性。P0.05被认为有统计学意义。结果1.SLE组和健康对照组PBMCs中Atgs mRNA水平的比较:SLE组Becline-1,LC3和p62 mRNA水平均高于健康对照组,差异有统计学意义(Becline-1 mRNA:9.96×10~(-4) vs 7.38×10~(-4),P0.001;LC3 mRNA:4.04×10~(-5) vs 2.62×10~(-5),P0.001;p62 mRNA:9.51×10~(-4) vs 7.59×10~(-4),P=0.008);两组间mTOR mRNA(2.97×10~(-4) vs 3.31×10~(-4),P=0.080)水平无显著差异。2.SLE患者Becline-1 mRNA水平与SLEDAI及免疫学指标的相关性:Becline-1 mRNA与SLEDAI(r=0.480;P0.00),IgG(r=0.511;P0.001)及抗ds-DNA抗体(r=0.620;P0.001)正相关,与C3(r=-0.454;P0.001)负相关,与C4(r=-0.180;P=0.108)无相关性。3.SLE患者LC3 mRNA水平与SLEDAI及免疫学指标的相关性:LC3 mRNA与SLEDAI(r=0.350;P=0.001),IgG(r=0.594;P0.001)及抗ds-DNA抗体(r=0.439;P0.001)正相关,与C3(r=-0.470;P0.001),C4(r=-0.251;P=0.024)负相关。4.SLE患者p62 mRNA水平与SLEDAI及免疫学指标的相关性:p62 mRNA与SLEDAI(r=0.505;P0.001),IgG(r=0.542;P0.001)及抗ds-DNA抗体(r=0.631;P0.001)正相关,与C3(r=-0.383;P0.001)负相关,与C4(r=-0.215;P=0.054)无相关性。结论SLE患者PBMCs中存在自噬形成活跃与自噬降解受阻的现象,维持自噬水平的平衡可能有助于改善SLE疾病活动及免疫学紊乱状况。
[Abstract]:Objective to investigate the expression of autophagy associated genes (Atgs) mTORORline-1, LC3 and p62) in peripheral blood mononuclear cells (PBMC) of (SLE) patients with systemic lupus erythematosus (SLE), and to analyze their correlation with SLE disease activity (SLEDAI) and immunological parameters. To explore the role of mTORline-1 LC3 and p62 in the development of SLE. Methods 1.81 SLE patients in the Department of Rheumatology and Immunology, the first affiliated Hospital of Zhengzhou University, were selected as experimental group. The clinical, demographic and laboratory data of all subjects were collected. In the same period, 86 healthy volunteers from the Department of physical examination of the first affiliated Hospital of Zhengzhou University were healthy controls. The mRNA levels of Atgs mTORORline-1 LC3 and p62 in 81 patients with SLE and 86 healthy controls were detected by RT-PCR. To analyze the difference of Atgs expression between SLE group and healthy control group and its correlation with SLEDAI and immunological indexes. Statistical analysis software SPSS 21.0, mean 卤standard deviation (?) 卤S) and percentage (%) were used to represent quantitative and qualitative data respectively. T test was used to compare quantitative data between two samples, and single factor analysis of variance (ANOVA) or nonparametric test was used to compare multigroup quantitative data. Qualitative data were compared with 蠂 2 test. Persons or Spearman rank correlation analysis was used to evaluate the correlation between Atgs mRNA level and SLEDAI and immunological indexes. P05 was considered to have statistical significance. Results the levels of Atgs mRNA in PBMCs in 1.SLE group and healthy control group were higher than those in healthy control group. 宸紓鏈夌粺璁″鎰忎箟(Becline-1 mRNA:9.96脳10~(-4) vs 7.38脳10~(-4),P0.001;LC3 mRNA:4.04脳10~(-5) vs 2.62脳10~(-5),P0.001;p62 mRNA:9.51脳10~(-4) vs 7.59脳10~(-4),P=0.008);涓ょ粍闂磎TOR mRNA(2.97脳10~(-4) vs 3.31脳10~(-4),P=0.080)姘村钩鏃犳樉钁楀樊寮，
本文编号：2187285
[Abstract]:Objective to investigate the expression of autophagy associated genes (Atgs) mTORORline-1, LC3 and p62) in peripheral blood mononuclear cells (PBMC) of (SLE) patients with systemic lupus erythematosus (SLE), and to analyze their correlation with SLE disease activity (SLEDAI) and immunological parameters. To explore the role of mTORline-1 LC3 and p62 in the development of SLE. Methods 1.81 SLE patients in the Department of Rheumatology and Immunology, the first affiliated Hospital of Zhengzhou University, were selected as experimental group. The clinical, demographic and laboratory data of all subjects were collected. In the same period, 86 healthy volunteers from the Department of physical examination of the first affiliated Hospital of Zhengzhou University were healthy controls. The mRNA levels of Atgs mTORORline-1 LC3 and p62 in 81 patients with SLE and 86 healthy controls were detected by RT-PCR. To analyze the difference of Atgs expression between SLE group and healthy control group and its correlation with SLEDAI and immunological indexes. Statistical analysis software SPSS 21.0, mean 卤standard deviation (?) 卤S) and percentage (%) were used to represent quantitative and qualitative data respectively. T test was used to compare quantitative data between two samples, and single factor analysis of variance (ANOVA) or nonparametric test was used to compare multigroup quantitative data. Qualitative data were compared with 蠂 2 test. Persons or Spearman rank correlation analysis was used to evaluate the correlation between Atgs mRNA level and SLEDAI and immunological indexes. P05 was considered to have statistical significance. Results the levels of Atgs mRNA in PBMCs in 1.SLE group and healthy control group were higher than those in healthy control group. 宸紓鏈夌粺璁″鎰忎箟(Becline-1 mRNA:9.96脳10~(-4) vs 7.38脳10~(-4),P0.001;LC3 mRNA:4.04脳10~(-5) vs 2.62脳10~(-5),P0.001;p62 mRNA:9.51脳10~(-4) vs 7.59脳10~(-4),P=0.008);涓ょ粍闂磎TOR mRNA(2.97脳10~(-4) vs 3.31脳10~(-4),P=0.080)姘村钩鏃犳樉钁楀樊寮，
本文编号：2187285
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