硫辛酸对骨质疏松大鼠骨代谢的影响及机制研究

发布时间：2018-08-17 15:56

【摘要】：目的探讨氧化应激及抗氧化剂硫辛酸在大鼠骨质疏松发生中的作用及可能的机制。方法选用8周龄Wistar雌性大鼠24只,随机分为对照组、骨质疏松组和硫辛酸干预组,采用双侧卵巢切除法建立骨质疏松大鼠模型,硫辛酸组采用60 mg/kg硫辛酸腹腔注射,干预共8周;检测骨密度,全血碱性磷酸酶(ALP),骨钙素,钙、磷水平,股骨组织MDA、SOD和GSH-Px含量;Western blotting方法测定股骨组织护骨素(OPG)和细胞核因子κB受体活化因子配基(RANKL)蛋白含量,Real-time PCR方法测定股骨组织OPG和RANKL m RNA表达水平。结果骨质疏松组大鼠骨密度,股骨组织中SOD、GSH-Px、OPG m RNA及蛋白表达低于对照组大鼠(P0.05);血清骨钙素,ALP水平,股骨组织中MDA、RANKL m RNA及蛋白水平都显著高于对照组大鼠(P0.05)。硫辛酸组大鼠骨密度和股骨组织中SOD、GSH-Px、OPG m RNA及蛋白表达高于骨质疏松组大鼠(P0.05);血清骨钙素,ALP水平,股骨组织中MDA、RANKL m RNA及蛋白表达水平都显著低于骨质疏松组大鼠(P0.05)。结论氧化应激可能通过OPG/RANKL通路促进了大鼠骨质疏松的发生,而抗氧化剂硫辛酸可缓解骨质疏松的进展。
[Abstract]:Objective to investigate the role and possible mechanism of oxidative stress and antioxidant lipoic acid in the pathogenesis of osteoporosis in rats. Methods Twenty-four 8-week-old female Wistar rats were randomly divided into control group, osteoporosis group and lipoic acid intervention group. The model of osteoporosis was established by bilateral ovariectomy, and the rats in the lipoic acid group were intraperitoneally injected with lipoic acid for 60 mg/kg. Bone mineral density (BMD), whole blood alkaline phosphatase (ALP),) osteocalcin, calcium and phosphorus levels were measured for 8 weeks. The content of MDA-SOD and GSH-Px in femur tissue was determined by Western blotting. The expression of OPG and RANKL m RNA in femur tissue was determined by real-time PCR and (OPG) of osteoprotegerin and nuclear factor 魏 B receptor activating factor ligand (RANKL) protein were determined by real-time PCR. Results the bone mineral density (BMD), the expression of SODH-PxPxOPG m RNA and protein in femur in osteoporosis group were significantly lower than those in control group (P0.05), and the levels of serum osteocalcin (BGP) and MDA-RANKL m RNA and protein in femur were significantly higher than those in control group (P0.05). The bone mineral density (BMD) and the expression of SODH-PxPxOPG m RNA and protein in femur tissue of rats in lipoic acid group were significantly higher than those in osteoporosis group (P0.05), while the levels of serum osteocalcin and RNA and protein in femur tissue were significantly lower than those in osteoporosis group (P0.05). Conclusion oxidative stress may promote the development of osteoporosis in rats through OPG/RANKL pathway, and the antioxidant lipoic acid can alleviate the progression of osteoporosis.
【作者单位】：沈阳医学院附属中心医院骨外三科;
【分类号】：R580

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