α-平滑肌肌动蛋白及转化生长因子-β1在高尿酸血症大鼠肾小管间质纤维化中的表达

发布时间：2018-08-18 09:00

【摘要】：目的观察α-平滑肌肌动蛋白(α-SMA)及转化生长因子-β1(TGF-β1)在高尿酸血症(HUA)大鼠肾小管间质纤维化(RIF)过程中的表达及意义。方法 SD大鼠36只,随机分为对照组(Con)、模型组及非布司他组,每组12只。氧嗪酸钾(OP)溶于0.5%的羧甲基纤维素钠(CMC)溶液,模型组和非布司他组用OP灌胃,1次/d,制备HUA模型,非布司他组另予非布司他灌胃,Con组仅予等量0.5%(CMC灌胃。于0、4、8周后检测血尿酸(SUA)、血肌酐(Scr)及BUN,收集24 h尿量,检测24 h尿蛋白定量。第0、4、8周末每组随机处死4只大鼠,HE染色及Masson染色观察肾组织病理变化,免疫组织化学法检测α-SMA、TGF-β1的表达。结果实验4周后,与Con组比较,模型组SUA、Scr、BUN升高(P0.05或P0.01),TGF-β1、α-SMA表达量增多(P0.01),肾小管间质发生纤维化。实验8周后,RIF加重;非布司他组RIF较模型组减轻(P0.01)。结论 HUA诱导肾组织TGF-β1、α-SMA表达量增加;α-SMA及TGF-β1表达增多促进RIF发生。
[Abstract]:Objective to investigate the expression of 伪 -SMA and transforming growth factor- 尾 1 (TGF- 尾 1) in renal tubulointerstitial fibrosis (RIF) in hyperuricemia (HUA) rats. Methods Thirty-six Sprague-Dawley rats were randomly divided into two groups: control group (Con), model group) and control group (control group) with 12 rats in each group. Potassium oxazinate (OP) was dissolved in 0.5% (CMC) solution of carboxymethyl cellulose sodium. The model group and non-bustrast group were fed with op for one time per day to make HUA model. In non-bustrast group, the same amount (CMC) was only given to the control group. The levels of serum creatinine (Scr) and bun in serum (SUA), were detected after 8 weeks. The urine volume was collected for 24 h and the urine protein was measured at 24 h. At the end of the 8th week, four rats in each group were randomly killed to observe the pathological changes of renal tissue by HE staining and Masson staining, and the expression of 伪 -SMA-TGF- 尾 1 was detected by immunohistochemistry. Results after 4 weeks, compared with Con group, the expression of TGF- 尾 1, 伪 -SMA and tubulointerstitial fibrosis in model group were increased (P0.05 or P0.01). After 8 weeks of experiment, RIF was aggravated, and RIF in non-busulta group was lighter than that in model group (P0. 01). Conclusion the expression of TGF- 尾 1 and 伪 -SMA in renal tissue was increased by HUA, and the expression of 伪 -SMA and TGF- 尾 1 was increased in renal tissue, and the expression of 伪 -SMA and TGF- 尾 1 promoted the occurrence of RIF.
【作者单位】：南方医科大学研究生学院;解放军第305医院内分泌科;
【基金】：解放军总参军事医学和老年病科研基金(ZCWS14B12)
【分类号】：R589.7;R692

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