类风湿性关节炎患者嗜碱性粒细胞活化机制研究
发布时间：2018-08-20 13:46
【摘要】：目的:探讨类风湿性关节炎患者外周血嗜碱性粒细胞的活化情况及其机制。方法:流式检测正常人对照组和RA患者组外周嗜碱性粒细胞活化标记物CD203c的表达及IL-4阳性的比例;用电化学发光法检测血清IgE水平;负选正常人嗜碱性粒细胞,以anti-IgE刺激为阳性对照,置含或不含IgE的微环境中,流式检测CD203c的表达及IL-4阳性的比例。结果:较正常人对照组,RA患者嗜碱性粒细胞高表达CD203c和IL-4(均P0.05);RA患者血清IgE水平显著高于正常人对照组(P0.05);RA患者血清和其血清中纯化的IgE可使负选的正常人来源的嗜碱性粒细胞活化,上调CD203c表达及IL-4阳性比例。结论:RA患者嗜碱性粒活化与发病相关,且主要由IgE介导,靶向嗜碱性粒细胞及其活化通路有望为RA的防治提供新的策略。
[Abstract]:Objective: to investigate the activation and mechanism of peripheral blood basophil in patients with rheumatoid arthritis. Methods: flow cytometry was used to detect the expression of peripheral basophil activation marker (CD203c) and the proportion of IL-4 positive in peripheral eosinophil activation marker in normal control group and RA group. The serum IgE level was detected by electrochemiluminescence assay. Using anti-IgE stimulation as positive control, the expression of CD203c and the proportion of IL-4 positive were detected by flow cytometry in microenvironment with or without IgE. Results: the expression of CD203c and IL-4 in serum of RA patients was significantly higher than that of normal controls (P0.05). The serum IgE levels in RA patients were significantly higher than those in normal controls (P0.05). The purified IgE in serum of RA patients and their sera could result in negative selection of eosinophils from normal people. Alkaline granulocyte activation, The expression of CD203c and the positive rate of IL-4 were upregulated. Conclusion the activation of basophil is related to the pathogenesis of RA and is mainly mediated by IgE. Targeting basophil and its activation pathway may provide a new strategy for the prevention and treatment of RA.
【作者单位】： 广东医学院附属医院临床医学研究中心;广东医学院附属医院风湿免疫科;广东医学院附属医院肾脏疾病研究所;
【基金】：国家自然科学基金(No.81471530) 广东省自然科学基金(No.S2013010011568)
【分类号】：R593.22
[Abstract]:Objective: to investigate the activation and mechanism of peripheral blood basophil in patients with rheumatoid arthritis. Methods: flow cytometry was used to detect the expression of peripheral basophil activation marker (CD203c) and the proportion of IL-4 positive in peripheral eosinophil activation marker in normal control group and RA group. The serum IgE level was detected by electrochemiluminescence assay. Using anti-IgE stimulation as positive control, the expression of CD203c and the proportion of IL-4 positive were detected by flow cytometry in microenvironment with or without IgE. Results: the expression of CD203c and IL-4 in serum of RA patients was significantly higher than that of normal controls (P0.05). The serum IgE levels in RA patients were significantly higher than those in normal controls (P0.05). The purified IgE in serum of RA patients and their sera could result in negative selection of eosinophils from normal people. Alkaline granulocyte activation, The expression of CD203c and the positive rate of IL-4 were upregulated. Conclusion the activation of basophil is related to the pathogenesis of RA and is mainly mediated by IgE. Targeting basophil and its activation pathway may provide a new strategy for the prevention and treatment of RA.
【作者单位】： 广东医学院附属医院临床医学研究中心;广东医学院附属医院风湿免疫科;广东医学院附属医院肾脏疾病研究所;
【基金】：国家自然科学基金(No.81471530) 广东省自然科学基金(No.S2013010011568)
【分类号】：R593.22
【相似文献】
相关期刊论文 前10条
1 杨玲;许以平;曹玲仙;王群;;不同浓度肽聚糖、脂多糖、聚肌胞苷酸对过敏性支气管哮喘患者嗜碱性粒细胞释放能力的作用[J];上海医学;2008年12期
2 冯永民;潘庆军;刘华锋;;嗜碱性粒细胞活化机制的研究进展[J];中国免疫学杂志;2013年08期
3 钱R季，
本文编号：2193831
本文链接：https://www.wllwen.com/yixuelunwen/nfm/2193831.html
最近更新
教材专著
