慢性氟中毒大鼠肾组织Beclin1、LC3、p62、Bcl-2表达变化及意义

发布时间：2018-08-22 11:10

【摘要】：目的观察慢性氟中毒大鼠肾组织自噬相关因子微管相关蛋白1(Beclin1)、微管相关蛋白1轻链3(LC3)、p62及抗凋亡因子B细胞淋巴瘤基因/白血病基因-2(Bcl-2)表达变化,探讨自噬及凋亡在慢性氟中毒肾脏损伤中的意义。方法选择健康SD大鼠36只,随机分为对照组、低氟组、高氟组,每组各12只。对照组饮用含氟化钠低于1 mg/L的自来水;低、高氟组饮用含氟化钠分别为5、50 mg/L的自来水,干预6个月建立氟中毒模型。干预结束后股动脉放血处死大鼠,取肾组织,光镜下观察肾组织病理形态学改变,免疫组织化学及RT-PCR法检测肾组织Beclin1、LC3、p62、Bcl-2蛋白和mRNA表达。结果 HE结果示,对照组大鼠肾脏肾小球及肾小管结构无明显变化,肾小管上皮细胞排列整齐。低氟组、高氟组大鼠肾小管上皮细胞有不同程度的水肿,管腔狭窄,以高氟组肾小管中间质充血更为明显。与对照组比较,低氟组、高氟组肾组织中Beclin1、LC3蛋白及mRNA表达均升高,p62、Bcl-2蛋白及mRNA表达均下降(P均0.05)。高氟组Beclin1、LC3蛋白及mRNA表达均高于低氟组,p62、Bcl-2蛋白及mRNA表达均低于低氟组(P均0.05)。结论慢性氟中毒后大鼠肾组织自噬及凋亡均激活,且其激活程度与氟中毒的程度有关,可能共同参与了慢性氟中毒肾损伤的发病机制。
[Abstract]:Objective to observe the expression of autophagy associated factor microtubule-associated protein 1 (Beclin1), microtubule-associated protein 1 light chain 3 (LC3) p62 and anti-apoptotic factor B cell lymphoma gene / leukemia gene 2 (Bcl-2) in chronic fluorosis rats. To investigate the significance of autophagy and apoptosis in renal injury caused by chronic fluorosis. Methods 36 healthy SD rats were randomly divided into control group, low fluorine group and high fluorine group with 12 rats in each group. The control group drank tap water containing sodium fluoride for less than 1 mg/L, while the high fluorine group drank the tap water containing sodium fluoride for 550 mg/L, and the model of fluorosis was established after 6 months of intervention. At the end of intervention, the rats were sacrificed by femoral artery bloodletting, the renal tissues were taken, the pathological changes of renal tissue were observed under light microscope, the expression of Bcl-2 protein and mRNA in renal tissue were detected by immunohistochemical method and RT-PCR method. Results the results of HE showed that the renal glomeruli and renal tubules in the control group had no obvious changes, and the renal tubular epithelial cells were arranged neatly. In low fluoride group and high fluoride group, renal tubular epithelial cells were edema and lumen stenosis, especially in high fluorine group. Compared with the control group, the expression of Beclin1 + LC3 protein and mRNA in renal tissue of low fluorine group and high fluorine group were significantly higher than those of control group (P < 0. 05). The expression of Beclin1 + LC3 protein and mRNA in high fluorine group was higher than that in low fluorine group. The expression of Bcl-2 protein and mRNA were lower in high fluorine group than in low fluoride group (P 0.05). Conclusion autophagy and apoptosis of renal tissue were activated after chronic fluorosis in rats, and the degree of activation was related to the degree of fluorosis, which may be involved in the pathogenesis of renal injury caused by chronic fluorosis.
【作者单位】：贵州医科大学;
【分类号】：R595

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