腺苷酸活化蛋白激酶（AMPKα2）基因多态性与2型糖尿病及颈动脉粥样硬化

发布时间：2018-08-22 17:34

【摘要】：研究目的:探讨腺苷酸活化蛋白激酶(AMPKα2)基因多态性与2型糖尿病及颈动脉粥样硬化之间的相关性研究对象:选择2012年12月至2014年12月在延边大学附属医院内分泌科住院的2型糖尿病患者209例。根据是否伴有颈动脉硬化分为2组,即2型糖尿病无颈动脉粥样硬化组89例和2型糖尿病颈动脉硬化组120例。健康人群67例作为正常对照组。研究方法:颈动脉IMT是通过彩色多普勒超声来测定的。将基因经过DNA进行扩增、纯化,并进行DNA测序及基因分型。运用Hardy-Weinberg平衡定律检验,确认研究样本的群体代表性,并将所有的数据进行录入,建立数据库,然后应用SPSS19.0统计软件进行分析,计量资料以均数±标准差来表示,在线http://analysis.bio-X.cn/myAnalysis.php计算等位基因型频率、基因型分布差异,多组间计量资料的比较采用单因素方差分析,其中两两比较采用LSD法比较,应用Lo gistic回归分析筛选颈动脉粥样硬化的危险因素,以P0.05为有统计学意义。结果:1.延边地区对照组T等位基因频率为77.6%,G等位基因频率为22.4%,2型糖尿病无颈动脉硬化组T等位基因频率65.2%,G等位基因频率为34.8%,2型糖尿病无颈动脉硬化组T等位基因频率比对照组低,G等位基因频率比对照组高,两组间进行比较存在统计学差异(P0.05),对照组TT基因型频率为58.2%,TG和GG基因型频率为38.8%、3.0%,2型糖尿病无颈动脉硬化组TT基因型频率40.4%,TG和GG基因型频率分别为49.4%、10.1%,2型糖尿病无颈动脉硬化组TT基因型频率较对照组低,TG和GG基因型频率相对于对照组高,两组间比较存在统计学差异(P0.05);2型糖尿病颈动脉硬化组T等位基因频率65.8%,G等位基因频率为34.2%,2型糖尿病颈动脉硬化组T等位基因频率较对照组低,G等位基因频率较对照组高,两组间比较存在统计学差异(P0.05),2型糖尿病颈动脉硬化组TT基因型频率40.0%,TG及GG基因型频率分别为51.7%、8.3%,2型糖尿病颈动脉硬化组TT基因型频率低于对照组,TG基因型和GG基因型频率高于对照组,两组间比较存在统计学差异(P0.05);2型糖尿病无颈动脉硬化组基因型及等位基因频率分布与2型糖尿病颈动脉硬化组比较不存在统计学差异(P0.05)。2.2型糖尿病患者中TT基因型组的低密度脂蛋白胆固醇水平2.90±1.36 mmol/L,糖化血红蛋白水平为8.31 ±2.19 mmol/L,GG基因型组低密度脂蛋白胆固醇水平3.01±1.01mmol/L,糖化血红蛋白水平为8.99±2.06mmol/L2型糖尿病患者中GG基因型组低密度脂蛋白胆固醇及糖化血红蛋白水平均高于TT基因型组。3.颈动脉粥样硬化的危险因素比较中,收缩压,低密度脂蛋白胆固醇,糖化血红蛋白的回归系数分别为0.017、0.281、0.194,高密度脂蛋白胆固醇的回归系数为-0.573。结论:1.延边地区AMPKα2基因rs1124900多态性与延边地区2型糖尿病有关。2.AMPKα2 rs1124900基因型G等位基因是2型糖尿病的易感基因。3.糖化血红蛋白,低密度脂蛋白胆固醇,收缩压为颈动脉粥样硬化的危险性因素,高密度脂蛋白胆固醇是保护性因素。
[Abstract]:AIM: To investigate the association between adenylate-activated protein kinase (AMPK alpha 2) gene polymorphisms and type 2 diabetes mellitus and carotid atherosclerosis in 209 patients with type 2 diabetes who were hospitalized in the Department of Endocrinology, Affiliated Hospital of Yanbian University from December 2012 to December 2014. Methods: The carotid IMT was detected by color Doppler ultrasound. The gene was amplified, purified, sequenced and genotyped. Hardy-Weinberg equilibrium test was used. To confirm the population representativeness of the study sample, input all the data, establish a database, and then use SPSS19.0 statistical software for analysis. The measurement data are expressed as mean (+) standard deviation. On-line http://analysis.bio-X.cn/myAnalysis.php is used to calculate allele frequencies, genotype distribution differences, and the ratios of multi-group measurement data. Results: 1. The T allele frequency of Yanbian control group was 77.6%, G allele frequency was 22.4%, type 2 diabetes mellitus without carotid atherosclerosis group T allele frequency was 22.4%. Because the frequency of T allele was 65.2%, the frequency of G allele was 34.8%. The frequency of T allele was lower and G allele was higher in type 2 diabetes mellitus without carotid atherosclerosis than that in control group. There was statistical difference between the two groups (P 0.05). The frequency of TT genotype in control group was 58.2%, the frequency of TG and GG genotype was 38.8%, 3.0%, and type 2 diabetes mellitus without carotid atherosclerosis. TT genotype frequencies were 40.4%, TG and GG genotype frequencies were 49.4% and 10.1%, respectively. TT genotype frequencies in type 2 diabetes mellitus without carotid atherosclerosis group were lower than those in control group, TG and GG genotype frequencies were higher than those in control group, and there were significant differences between the two groups (P 0.05); T allele frequencies were 65.8% and G allele frequencies in type 2 diabetic carotid atheroscle The frequency of T allele and G allele in type 2 diabetes mellitus with carotid atherosclerosis was 34.2%. The frequency of TT genotype was 40.0% in type 2 diabetes with carotid atherosclerosis, 51.7% in TG and 8.3% in type 2 diabetes with carotid atherosclerosis. The frequency of TG genotype and GG genotype was higher than that of the control group (P 0.05). The distribution of genotype and allele frequency in type 2 diabetes mellitus without carotid atherosclerosis group was not significantly different from that in type 2 diabetes mellitus with carotid atherosclerosis group (P 0.05). 2. The levels of LDL-C, HbA1c, LDL-C and HbA1c in GG genotype, GG genotype, GG genotype and HbA1c in GG genotype were higher than those in TT genotype and GG genotype. The regression coefficients of systolic blood pressure, low density lipoprotein cholesterol and glycosylated hemoglobin were 0.017, 0.281 and 0.194, respectively. The regression coefficients of high density lipoprotein cholesterol were - 0.573. Conclusion: 1. The rs1124900 polymorphism of AMPK alpha2 gene was associated with type 2 diabetes mellitus in Yanbian area. Glycosylated hemoglobin, low density lipoprotein cholesterol, systolic blood pressure are risk factors for carotid atherosclerosis, and high density lipoprotein cholesterol is a protective factor.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.2

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