糖尿病大鼠胃平滑肌内质网应激相关蛋白动态变化及其意义的研究

发布时间：2018-09-18 16:33

【摘要】：目的观察STZ诱导糖尿病大鼠胃平滑肌内质网应激相关蛋白GRP-78、CHOP、Caspase-12及其效应蛋白Bcl-2、Caspase-3的变化,探讨其在糖尿病胃轻瘫中发生的意义。方法根据STZ诱导6周大鼠出现糖尿病胃轻瘫症状及胃平滑肌细胞出现凋亡的前期实验结果,将SD大鼠分为正常对照组(NC)组和模型6周组(DM6W)。Western blot检测两组胃平滑肌GRP-78、CHOP、Caspase-12的表达,免疫组织化学染色检测两组胃平滑肌Bcl-2、Caspase-3的表达。结果 NC组胃平滑肌GRP-78、CHOP、Caspase-12的相对表达值低于DM6W组[(0.12±0.03)vs(0.23±0.03),(0.11±0.02)vs(0.24±0.03),(0.11±0.03)vs(0.24±0.04),P0.01];NC组胃平滑肌Bcl-2的表达高于DM6W组[(491.91±22.96)vs(159.48±20.29),P0.01];NC组胃平滑肌Caspase-3的表达低于DM6W组[(154.15±10.03)vs(489.45±52.08),P0.01]。结论糖尿病胃轻瘫大鼠胃平滑肌存在内质网应激,并且参与诱导胃平滑细胞凋亡。
[Abstract]:Objective to observe the changes of endoplasmic reticulum stress-related protein (GRP-78,CHOP,Caspase-12) and its effector protein (Bcl-2,Caspase-3) in gastric smooth muscle of diabetic rats induced by STZ, and to explore its significance in diabetic gastroparesis. Methods according to the experimental results of STZ induced diabetic gastroparesis and apoptosis of gastric smooth muscle cells, SD rats were divided into two groups: normal control group (NC) group) and model group (DM6W). Western blot was used to detect the expression of GRP-78,CHOP,Caspase-12 in gastric smooth muscle. The expression of Bcl-2,Caspase-3 in gastric smooth muscle was detected by immunohistochemical staining. Results the relative expression of GRP-78,CHOP,Caspase-12 in gastric smooth muscle of NC group was lower than that of DM6W group [(0.12 卤0.03) vs (0.23 卤0.03) vs (0.11 卤0.03) vs (0.24 卤0.03) vs (0.24 卤0.04) P0.01] the Bcl-2 expression of gastric smooth muscle in NC group was higher than that in DM6W group [(491.91 卤22.96) vs (159.48 卤20.29) P0.01] the expression of Caspase-3 in gastric smooth muscle in NC group was lower than that in DM6W group [(154.15 卤10.03) vs (489.45 卤52.08) P0.01]. Conclusion there is endoplasmic reticulum stress in gastric smooth muscle of diabetic gastroparesis rats and it is involved in inducing apoptosis of gastric smooth cells.
【作者单位】：延边大学医学院组织学与胚胎学教研室;
【基金】：国家自然科学基金(81360070,81560142)
【分类号】：R587.2

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