大气颗粒物与花粉联合氧化损伤作用研究
发布时间：2018-09-19 11:15
【摘要】：目的空气颗粒物(PM)可通过多种途径导致花粉过敏症的发病率增加或过敏症的症状加重,其作用机制可能与氧化损伤的加重有关。本研究目的是了解不同大气颗粒物和天津市夏、秋季的花粉等对中国仓鼠肺细胞的毒性水平,研究大气颗粒物的毒性损伤与花粉过敏反应的相互作用,为可能的空气污染物耦合健康损伤提供理论支持,同时为花粉过敏症的预防和控制提供理论依据。方法对天津市烟道灰、燃煤飞灰和锅底灰等颗粒物进行采样,并购买天津市夏、秋季优势花粉豚草、梯牧草和杨树花粉等作为实验样品,选取中国仓鼠肺细胞(CHL)作为实验对象。分别用颗粒物和花粉对细胞染毒,通过噻唑蓝(MTT)比色法检测各样品不同浓度的细胞毒性。将花粉、颗粒物以及花粉和颗粒物的混合物对细胞染毒,分别检测细胞和细胞培养基的氧化损伤指标,包括:丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GSH-PX)活力等,比较花粉和颗粒物的混合物对细胞造成的氧化损伤是否高于单一污染物。结果1.颗粒物的细胞毒性:在实验浓度(12.5μg/ml~200μg/ml)范围内,颗粒物对中国仓鼠肺细胞(CHL)的生长的抑制率随染毒剂量增加而增加。当颗粒物样品的染毒剂量浓度为12.5μg/ml和25μg/ml时,燃煤飞灰和锅灰的细胞毒性相似(P0.05),而烟道灰的细胞毒性明显高于以上两种(P0.05)。随染毒剂量浓度升高至50μg/ml和100μg/ml时,燃煤飞灰和烟道灰表现出相似的细胞毒性(P0.05),而锅灰的细胞毒性明显小于上述两种(P0.05)。当染毒剂量浓度上升至200μg/ml时,燃煤飞灰、锅灰和烟道灰三种颗粒物对细胞生长的机制作用未显示出统计学差异(P0.05)。2.花粉的细胞毒性:在实验浓度(12.5μg/ml~200μg/ml)范围内,花粉对中国仓鼠肺细胞(CHL)的生长抑制率随染毒剂量增加而增加。在实验所用染毒浓度范围内,相同浓度的杨树和豚草表现出相似的细胞毒性(P0.05),当花粉染毒剂量浓度为12.5μg/ml时,梯牧草花粉的细胞浓度显著低于豚草花粉和杨树花粉(P0.05),而当染毒剂量浓度为50μg/ml~200μg/ml时,梯牧草花粉对细胞生长的抑制率显著高于豚草花粉和杨树花粉(P0.05)。3.颗粒物和花粉的氧化损伤作用:颗粒物和混合物染毒组之间的丙二醛(MDA)含量对比空白组MDA含量均明显增加(P0.05);对比豚草花粉单独染毒或燃煤飞灰颗粒物单独染毒,花粉和颗粒物混合物染毒组的MDA含量明显升高(P0.05)。对比空白组总超氧化物歧化酶(T-SOD)活性,豚草花粉、燃煤飞灰颗粒物以及豚草花粉和燃煤飞灰颗粒物混合物染毒组的T-SOD活性显著降低(P0.05);然而对比豚草花粉单独染毒或燃煤飞灰颗粒物单独染毒,豚草花粉和燃煤飞灰颗粒物混合物染毒组的T-SOD活性随呈现下降趋势,但未显示出统计学差异(P0.05)。对比空白组的谷胱甘肽过氧化物酶(GSH-PX)活性,豚草花粉、燃煤飞灰颗粒物以及豚草花粉和燃煤飞灰颗粒物混合物染毒组的GSH-PX活性均呈现下降趋势,然而仅有培养基中燃煤飞灰染毒组存在统计学差异(P0.05),其余各组均不存在统计学差异(P0.05);分别对比豚草花粉或燃煤飞灰单独染毒,豚草花粉和燃煤飞灰颗粒物混合物染毒组中GSH-PX活性随呈现下降趋势,但不存在统计学差异(P0.05)。说明豚草花粉和燃煤飞灰颗粒物均可造成CHL细胞的氧化损伤,并且当豚草花粉和燃煤飞灰颗粒物联合作用是,其氧化损伤作用有增强的趋势。结论颗粒物和花粉对细胞均表现出不同的细胞毒性。对比颗粒物或花粉的单一作用,颗粒物和花粉的联合作用可增加对细胞的氧化损伤。
[Abstract]:Objective Air particulate matter (PM) can increase the incidence of pollen allergy or aggravate the symptoms of allergy through various ways. The mechanism may be related to the aggravation of oxidative damage. The interaction between toxic damage of particles and pollen allergic reaction provides theoretical support for the possible coupling health damage of air pollutants and provides theoretical basis for the prevention and control of pollen allergy. Chinese hamster lung cells (CHL) were selected as the experimental subjects. Cell toxicity was detected by MTT colorimetry. Pollen, particulate matter and the mixture of particulate matter and particulate matter were poisoned to cells, respectively. The content of MDA, SOD and GSH-PX were measured to compare the oxidative damage caused by the mixture of pollen and particulate matter. The inhibitory rate of particulate matter on the growth of Chinese hamster lung cells (CHL) increased with the increase of the dosage in the range of 12.5 to 200 ug/ml. When the dosage of particulate matter was 12.5 and 25 ug/ml, the cytotoxicity of Coal-fired Fly Ash and boiler ash was similar (P 0.05), but the cytotoxicity of flue ash was significantly higher than the above two (P 0.05). The cytotoxicity of fly ash and flue ash was similar (P 0.05) when the dosage was increased to 50 ug/ml and 100 ug/ml. The cytotoxicity of fly ash and flue ash was significantly lower than that of the above two (P 0.05). Pollen cytotoxicity: The growth inhibition rate of pollen on Chinese hamster lung cells (CHL) increased with the increase of exposure dose in the range of experimental concentration (12.5 to 200 ug/ml). Poplar and ragweed at the same concentration showed similar cytotoxicity in the range of experimental concentration (P 0.05). The cell concentration of T. terminalis pollen was significantly lower than that of ragweed pollen and poplar pollen (P 0.05) when the dose was 12.5 ug/ml, but the inhibition rate of T. terminalis pollen on cell growth was significantly higher than that of ragweed pollen and poplar pollen (P 0.05). 3. Oxidative damage of particles and pollen: particles Compared with the blank group, the content of MDA increased significantly (P 0.05); compared with the control group, the content of MDA increased significantly (P 0.05) in the pollen alone or in the fly ash particulate matter alone. Compared with the blank group, the total superoxide dismutase (T-SOD) activity in the pollen and particulate matter mixture group was significantly higher (P 0.05). T-SOD activity in the groups exposed to powder, fly ash particulate matter and the mixture of ragweed pollen and fly ash particulate matter decreased significantly (P The activity of glutathione peroxidase (GSH-PX) in the blank group, ragweed pollen, Coal-fired Fly ash particulate matter and the mixture of ragweed pollen and coal-fired fly ash particulate matter all showed a downward trend. However, only the Coal-fired Fly Ash group in the culture medium had a significant difference (P 0.05). There was no significant difference between the two groups (P 0.05). Compared with the single exposure to ragweed pollen or Coal-fired Fly ash, the GSH-PX activity of the mixture of ragweed pollen and coal-fired fly ash particles decreased, but there was no significant difference (P 0.05). It indicated that both ragweed pollen and coal-fired fly ash particles could cause oxidative damage to CHL cells, and there was no significant difference between the two groups. ConclusionParticles and pollens have different cytotoxicity to cells. Compared with the single effect of particulate matter or pollen, the combined effect of particulate matter and pollen can increase the oxidative damage to cells.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X513;R593.1
本文编号：2249972
[Abstract]:Objective Air particulate matter (PM) can increase the incidence of pollen allergy or aggravate the symptoms of allergy through various ways. The mechanism may be related to the aggravation of oxidative damage. The interaction between toxic damage of particles and pollen allergic reaction provides theoretical support for the possible coupling health damage of air pollutants and provides theoretical basis for the prevention and control of pollen allergy. Chinese hamster lung cells (CHL) were selected as the experimental subjects. Cell toxicity was detected by MTT colorimetry. Pollen, particulate matter and the mixture of particulate matter and particulate matter were poisoned to cells, respectively. The content of MDA, SOD and GSH-PX were measured to compare the oxidative damage caused by the mixture of pollen and particulate matter. The inhibitory rate of particulate matter on the growth of Chinese hamster lung cells (CHL) increased with the increase of the dosage in the range of 12.5 to 200 ug/ml. When the dosage of particulate matter was 12.5 and 25 ug/ml, the cytotoxicity of Coal-fired Fly Ash and boiler ash was similar (P 0.05), but the cytotoxicity of flue ash was significantly higher than the above two (P 0.05). The cytotoxicity of fly ash and flue ash was similar (P 0.05) when the dosage was increased to 50 ug/ml and 100 ug/ml. The cytotoxicity of fly ash and flue ash was significantly lower than that of the above two (P 0.05). Pollen cytotoxicity: The growth inhibition rate of pollen on Chinese hamster lung cells (CHL) increased with the increase of exposure dose in the range of experimental concentration (12.5 to 200 ug/ml). Poplar and ragweed at the same concentration showed similar cytotoxicity in the range of experimental concentration (P 0.05). The cell concentration of T. terminalis pollen was significantly lower than that of ragweed pollen and poplar pollen (P 0.05) when the dose was 12.5 ug/ml, but the inhibition rate of T. terminalis pollen on cell growth was significantly higher than that of ragweed pollen and poplar pollen (P 0.05). 3. Oxidative damage of particles and pollen: particles Compared with the blank group, the content of MDA increased significantly (P 0.05); compared with the control group, the content of MDA increased significantly (P 0.05) in the pollen alone or in the fly ash particulate matter alone. Compared with the blank group, the total superoxide dismutase (T-SOD) activity in the pollen and particulate matter mixture group was significantly higher (P 0.05). T-SOD activity in the groups exposed to powder, fly ash particulate matter and the mixture of ragweed pollen and fly ash particulate matter decreased significantly (P The activity of glutathione peroxidase (GSH-PX) in the blank group, ragweed pollen, Coal-fired Fly ash particulate matter and the mixture of ragweed pollen and coal-fired fly ash particulate matter all showed a downward trend. However, only the Coal-fired Fly Ash group in the culture medium had a significant difference (P 0.05). There was no significant difference between the two groups (P 0.05). Compared with the single exposure to ragweed pollen or Coal-fired Fly ash, the GSH-PX activity of the mixture of ragweed pollen and coal-fired fly ash particles decreased, but there was no significant difference (P 0.05). It indicated that both ragweed pollen and coal-fired fly ash particles could cause oxidative damage to CHL cells, and there was no significant difference between the two groups. ConclusionParticles and pollens have different cytotoxicity to cells. Compared with the single effect of particulate matter or pollen, the combined effect of particulate matter and pollen can increase the oxidative damage to cells.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X513;R593.1
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