CRTH2受体拮抗剂对支气管哮喘树突状细胞功能的影响

发布时间：2018-10-05 13:47

【摘要】：目的培养支气管哮喘患儿外周血树突状细胞,体外给予CRTH2受体拮抗剂干预,从而探讨CRTH2受体在支气管哮喘发病中的作用。方法提取外周血单个核细胞,体外给于10 ng/ml GM-CSF及IL-4的完全培养液培养,并在第5天磁珠分选得到纯度90%以上的树突状细胞,体外给予CRTH2受体拮抗剂干预,收集细胞及上清,运用流式细胞技术检测DCs表型,ELISA方法检测细胞上清IL-10、IL-12含量变化,同种混合淋巴细胞反应检测树突状细胞刺激淋巴细胞增殖及分化的能力。结果经磁珠分选后,树突状细胞纯度可达90%,符合实验要求;给药组与对照组相比,给药组外周血树突状细胞表面表达的CD80、CD86、CD40显著增高(P0.05);给药组外周血树突状细胞分泌的IL-12明显高于对照组,IL-10明显低于对照组(P0.05);给药组外周血树突状细胞刺激T淋巴细胞增殖能力显著大于对照组(P0.05);给药组外周血树突状细胞刺激T淋巴细胞分泌IL-4含量明显低于对照组,IFN-γ含量明显高于对照组(P0.05)。结论与对照组相比,CRTH2受体可能通过调节树突状细胞的功能来达到参与疾病的发生发展。
[Abstract]:Objective to culture dendritic cells from peripheral blood of children with bronchial asthma and to investigate the role of CRTH2 receptor in the pathogenesis of bronchial asthma. Methods Peripheral blood mononuclear cells (PBMC) were isolated and cultured in vitro in 10 ng/ml GM-CSF and IL-4 culture medium. The dendritic cells with purity of more than 90% were isolated by magnetic beads on the 5th day. CRTH2 receptor antagonists were administered in vitro to collect the cells and supernatants. The changes of IL-10,IL-12 content in supernatant of DCs were detected by flow cytometry and the ability of dendritic cells to stimulate lymphocyte proliferation and differentiation was detected by homologous mixed lymphocyte reaction. Results the purity of dendritic cells was 90, which met the requirements of the experiment. The expression of CD80,CD86,CD40 on the surface of peripheral blood dendritic cells in the administration group was significantly higher than that in the control group (P0.05), the IL-12 secreted by the peripheral blood dendritic cells in the administration group was significantly higher than that in the control group (P0.05), and the peripheral blood dendritic cells stimulated the thin T-lymphoid cells in the administration group (P0.05). The cell proliferation ability was significantly higher than that of the control group (P0.05), and the IL-4 content of peripheral blood dendritic cells stimulated T lymphocytes was significantly lower than that of the control group (P0.05). Conclusion compared with the control group, CRTH2 receptor may be involved in the development of the disease by regulating the function of dendritic cells.
【作者单位】：唐山妇幼保健院儿内科;唐山妇幼保健院检验科;
【分类号】：R562.25

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