干燥综合征患者IL-27及其受体水平的研究
发布时间：2018-10-10 17:43
【摘要】：目的:通过检测pSS患者血清中IL-27的水平和外周血白细胞表面IL-27R(gp130和WSX-1)的表达水平,分析IL-27及其受体与pSS发病的关系,探讨IL-27及其受体在pSS发病中的意义。方法:(1)通过双抗体夹心酶联免疫吸附法来检测pSS组与正常对照组外周血血清中IL-27的水平,分析pSS患者血清中IL-27的水平与pSS发病的关系。(2)通过流式细胞术来测定pSS组与正常对照组外周血白细胞表面的IL-27R(gp130和WSX-1)的表达水平,分析pSS患者白细胞表面IL-27R(gp130和WSX-1)的表达水平和pSS发病的关系。结果:(1)pSS组血清中IL-27的水平明显高于正常对照组(161.25±22.75pg/ml VS 95.56±15.80pg/ml,p0.05);(2)pSS组外周血白细胞表面gp130(%)的表达水平(7.80±3.51)高于对照组(3.25±1.92)(p0.05),pSS组外周血白细胞表面WSX-1(%)的表达水平(2.91±0.99)高于对照组(1.45±0.85)(p0.05)。结论:pSS患者血清中的IL-27及白细胞表面的IL-27R(gp130和WSX-1)均存在异常表达,提示IL-27及其受体可能参加了pSS的发生、发展过程。
[Abstract]:Objective: to analyze the relationship between IL-27 and its receptor and pSS by detecting the level of IL-27 in serum and the expression of IL-27R (gp130 and WSX-1) on peripheral blood leukocytes in patients with pSS, and to explore the significance of IL-27 and its receptor in the pathogenesis of pSS. Methods: (1) double antibody sandwich enzyme linked immunosorbent assay (Elisa) was used to detect the level of IL-27 in peripheral blood of pSS group and normal control group. To analyze the relationship between the level of IL-27 in serum of pSS patients and the pathogenesis of pSS. (2) to detect the expression of IL-27R (gp130 and WSX-1) on the surface of peripheral blood leukocytes in pSS group and normal control group by flow cytometry. To analyze the relationship between the expression of IL-27R (gp130 and WSX-1) and the pathogenesis of pSS in patients with pSS. Results: (1) the level of serum IL-27 in pSS group was significantly higher than that in normal control group (161.25 卤22.75pg/ml VS 95.56 卤15.80 PG / ml 0.05); (2). The expression of gp130 (%) on peripheral white blood cell surface in pSS group was (7.80 卤3.51) higher than that in control group (3.25 卤1.92) (p0.05), pSS group). The expression level of WSX-1 (%) on peripheral blood leukocyte surface in pSS group was (2.91 卤0.99) higher than that in control group (1.45 卤0.85) (p0.05). Conclusion: the abnormal expression of IL-27 in serum and IL-27R (gp130 and WSX-1) on leukocytes in patients with pSS suggests that IL-27 and its receptors may be involved in the pathogenesis and development of pSS.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R593.2
本文编号：2262707
[Abstract]:Objective: to analyze the relationship between IL-27 and its receptor and pSS by detecting the level of IL-27 in serum and the expression of IL-27R (gp130 and WSX-1) on peripheral blood leukocytes in patients with pSS, and to explore the significance of IL-27 and its receptor in the pathogenesis of pSS. Methods: (1) double antibody sandwich enzyme linked immunosorbent assay (Elisa) was used to detect the level of IL-27 in peripheral blood of pSS group and normal control group. To analyze the relationship between the level of IL-27 in serum of pSS patients and the pathogenesis of pSS. (2) to detect the expression of IL-27R (gp130 and WSX-1) on the surface of peripheral blood leukocytes in pSS group and normal control group by flow cytometry. To analyze the relationship between the expression of IL-27R (gp130 and WSX-1) and the pathogenesis of pSS in patients with pSS. Results: (1) the level of serum IL-27 in pSS group was significantly higher than that in normal control group (161.25 卤22.75pg/ml VS 95.56 卤15.80 PG / ml 0.05); (2). The expression of gp130 (%) on peripheral white blood cell surface in pSS group was (7.80 卤3.51) higher than that in control group (3.25 卤1.92) (p0.05), pSS group). The expression level of WSX-1 (%) on peripheral blood leukocyte surface in pSS group was (2.91 卤0.99) higher than that in control group (1.45 卤0.85) (p0.05). Conclusion: the abnormal expression of IL-27 in serum and IL-27R (gp130 and WSX-1) on leukocytes in patients with pSS suggests that IL-27 and its receptors may be involved in the pathogenesis and development of pSS.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R593.2
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1 姜书霞;干燥综合征患者IL-27及其受体水平的研究[D];青海大学;2017年
，本文编号：2262707
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