孕期摄食限制对雌性子代老年大鼠代谢综合征易感的影响

发布时间：2018-10-11 07:50

【摘要】：目的探讨孕期摄食限制(prenatal food restriction,PFR)的雌性子代老年大鼠在经历体重追赶性生长和后期慢性应激后的代谢综合征(metabolic syndrome,Met S)易感现象及其可能的发生机制。方法将受孕的SPF级Wistar大鼠随机分为对照组和PFR组,每组18只。自孕11 d起至分娩,PFR组限制摄食(为对照组每日食物的50%),对照组自由饮食至自然分娩。分娩后每组筛选出窝仔数为10~12只的母鼠9窝,每窝随机抽取1只雌性仔鼠。仔鼠断乳后均给予高脂饮食至成年,出生后38周龄给予两周的预知性慢性应激,并采集应激前后仔鼠血清。以放射免疫法检测血清促肾上腺皮质激素(adreno-cortico-tropic-hormone,ACTH)和胰岛素水平,酶联免疫吸附法检测血清皮质酮(corticosterone,CORT)浓度,酶比色法检测血糖、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白-胆固醇(high-density lipoprotein,HDL-C)和低密度脂蛋白-胆固醇(low-density lipoprotein,LDL-C)浓度。结果与对照组相比,PFR组子代大鼠出生后体重偏低,但其出生后16~36周体重增长率升高。应激前,PFR组血清胰岛素、HDL-C水平降低,TC/HDL-C比值升高;应激后,PFR组血清ACTH无明显改变,CORT水平升高,血清胰岛素水平降低,血清TG水平和TG/HDL-C比值升高,差异均有统计学意义(P0.05或P0.01)。结论出生后早期经历追赶性生长的PFR雌鼠存在糖脂代谢紊乱及Met S易感,其发生可能与下丘脑-垂体-肾上腺(hypothalamic-pituitary-adrenal axis,HPA)轴相关的神经内分泌代谢编程紊乱有关。
[Abstract]:Objective to investigate the susceptibility and possible mechanism of metabolic syndrome (metabolic syndrome,Met S) in the elderly female offspring of gestational feeding restriction (prenatal food restriction,PFR) after body weight catch-up growth and late chronic stress. Methods pregnant SPF Wistar rats were randomly divided into control group and PFR group with 18 rats in each group. From the 11th day of pregnancy to delivery, the PFR group was restricted to feeding (50% of the daily food in the control group), and the control group was fed freely until the natural delivery. After delivery, 9 litters of 10 litters were selected from each group, and one female litter was randomly selected from each litter. Young rats were given a high-fat diet until adulthood after weaning and two weeks of predictive chronic stress were given at 38 weeks after birth and serum samples were collected before and after stress. Serum corticotropin (adreno-cortico-tropic-hormone,ACTH) and insulin levels were measured by radioimmunoassay, serum corticosterone (corticosterone,CORT) concentration was detected by enzyme linked immunosorbent assay (Elisa), and blood glucose was detected by enzyme colorimetry. Concentrations of total cholesterol (total cholesterol,TC), triglyceride (triglyceride,TG), high density lipoprotein cholesterol (high-density lipoprotein,HDL-C) and low density lipoprotein cholesterol (low-density lipoprotein,LDL-C). Results compared with the control group, the postnatal weight of the offspring of PFR group was lower than that of the control group, but the growth rate of body weight increased at 16 ~ 36 weeks after birth. Before stress, the levels of serum insulin, HDL-C and TC/HDL-C increased in PFR group, but no significant changes were found in serum ACTH, CORT level, insulin level, TG level and TG/HDL-C ratio in PFR group after stress (P0.05 or P0.01). Conclusion there is a disorder of glucose and lipid metabolism and susceptibility to Met S in PFR female rats with early postnatal catch-up growth, which may be related to the disorder of neuroendocrine metabolism related to the hypothalamus-pituitary-adrenal (hypothalamic-pituitary-adrenal axis,HPA) axis.
【作者单位】：武汉大学基础医学院药理学系;发育源性疾病湖北省重点实验室;湖北省随州市妇幼保健院;
【基金】：国家自然科学基金重大国际合作及重点项目(81220108026;81430089)
【分类号】：R589

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