GDF11对Ⅱ型糖尿病小鼠心肌损伤的保护作用

发布时间：2018-10-12 08:33

【摘要】：目的观察GDF11对Ⅱ型糖尿病C57BL/6J小鼠心肌损伤的保护作用及心肌凋亡水平的变化情况。方法 60只体重20~25 g的雄性C57BL/6J小鼠,随机分为3个组:正常对照组(control)、II型糖尿病模型组(DM)和GDF11干预组(DM+GDF11)。高脂高糖饲料饲养4周后,连续3次腹腔注射60 mg/kg链脲佐菌素(STZ),建立II型糖尿病小鼠模型,持续高脂高糖饲料饲养4周后,小动物超声检测心功能,取心肌组织,TUNEL染色检测心肌组织凋亡比例,Western blot法检测凋亡相关蛋白cleaved-caspase-3、Bcl-2和Bax的表达。结果糖尿病损伤显著下调左室射血分数和左室短轴缩短率,增加心肌凋亡率,而给予重组GDF11蛋白能够明显改善心功能并减轻心肌凋亡。结论外源性的GDF11可以显著减轻糖尿病损伤后心肌凋亡水平,改善心功能。
[Abstract]:Objective to observe the protective effect of GDF11 on myocardial injury and myocardial apoptosis in type 2 diabetic C57BL/6J mice. Methods 60 male C57BL/6J mice weighing 20 ~ 25 g were randomly divided into three groups: normal control group, (control), II type diabetes model group, (DM) group and GDF11 intervention group (DM GDF11). After fed with high fat and high sugar diet for 4 weeks, 60 mg/kg streptozotocin (STZ),) was injected intraperitoneally for 3 times to establish II diabetic model in mice. After feeding with high fat and high sugar diet for 4 weeks, cardiac function was detected by ultrasound in small animals. The expression of apoptosis-related proteins (cleaved-caspase-3,Bcl-2 and Bax) was detected by, Western blot method. Results Diabetic injury significantly decreased left ventricular ejection fraction (LVEF) and left ventricular short-axis shortening rate, and increased myocardial apoptosis rate. Administration of recombinant GDF11 protein could significantly improve cardiac function and reduce myocardial apoptosis. Conclusion exogenous GDF11 can significantly reduce myocardial apoptosis and improve cardiac function after diabetic injury.
【作者单位】：第四军医大学西京医院心血管外科;
【基金】：国家自然科学基金(81570230);国家自然科学基金(81570330) 国家“十二五”科技支撑计划课题(2011BAI11B20)
【分类号】：R542.2;R587.2

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