尿酸对人肾小管上皮细胞中PDZK1、PLXNB2和ZNF479的表达影响研究

发布时间：2018-10-19 13:26

【摘要】：目的:本研究旨在分析尿酸对肾小管上皮细胞中痛风易感基因PDZK1、PLXNB2、ZNF479表达的影响。方法:研究对象选取人肾小管上皮细胞(HK-2细胞)。体外培养后,用不同浓度尿酸(3 mg/dl,6 mg/dl,and 9 mg/dl)进行共培养,48小时收集细胞,提取RNA,然后反转录成c DNA后,荧光定量PCR检测PDZK1、PLXNB2和ZNF479基因的表达情况。结果:研究显示,随着尿酸浓度的变化,PDZK1及PLXNB2基因在HK-2细胞中表达成正相关变化关系(R=0.847,P=0.01;R=0.643,P=0.01)且差异有统计学意义(P=0.001,P=0.006)。9 mg/dl尿酸组PDZK1 m RNA表达量分别为对照组及3 mg/dl尿酸组的1.29倍(P=0.001)及1.21倍(P=0.007)。9 mg/dl尿酸组PLXNB2 m RNA表达量分别为对照组、3 mg/dl尿酸组及6 mg/dl尿酸组的1.49倍(P=0.02)、1.66倍(P=0.005)及1.4倍(P=0.042)。尿酸浓度与HK-2细胞中ZNF479基因的m RNA表达成正相关关系((R=0.568,P=0.05),但差异无统计学意义(P=0.155)。尿酸诱导HK-2细胞形态变长梭形或不规侧状。结论:尿酸能上调人肾小管上皮细胞中PDZK1,PLXNB2及ZNF479基因的m RNA表达。尿酸能诱导肾小管上皮细胞形态变长梭形或不规侧状。
[Abstract]:Aim: to investigate the effect of uric acid on the expression of gout susceptibility gene PDZK1,PLXNB2,ZNF479 in renal tubular epithelial cells. Methods: human renal tubular epithelial cells (HK-2 cells) were selected. After cultured in vitro, the cells were co-cultured with different concentrations of uric acid (3 mg/dl,6 mg/dl,and 9 mg/dl). The cells were collected for 48 hours. The RNA, was extracted and then inverted into c DNA. The expression of PDZK1,PLXNB2 and ZNF479 genes was detected by fluorescence quantitative PCR. Results: studies have shown that With the change of uric acid concentration, the expression of PDZK1 and PLXNB2 genes in HK-2 cells showed a positive correlation (P < 0.01), and the difference was statistically significant (P < 0.001). 9 the expression of PDZK1 m RNA in mg/dl uric acid group was 1.29 times (P0.001) and 1.21 fold (P0. 007) as compared with control group and 3 mg/dl uric acid group (P0. 007), and the expression of PDZK1 m RNA in mg/dl uric acid group was 1. 29 times (P0. 001) and 1. 21 times as much as that in 3 mg/dl uric acid group (P0. 007). The expression of PLXNB2 m RNA in mg/dl uric acid group was 1.49 fold (P0. 02), 1. 66 fold (P0. 005) and 1. 4 fold (P0. 042) of control group, 3 mg/dl uric acid group and 6 mg/dl uric acid group respectively. There was a positive correlation between the concentration of uric acid and the expression of m RNA of ZNF479 gene in HK-2 cells, but there was no significant difference (P0. 155). Uric acid induced long fusiform or irregular shape of HK-2 cells. Conclusion: uric acid can up-regulate m RNA expression of PDZK1,PLXNB2 and ZNF479 genes in human renal tubular epithelial cells. Uric acid can induce tubular epithelial cells to become long fusiform or irregular shape.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R589.7

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