应用原子力显微镜观察脉冲电磁场对大鼠骨质疏松的治疗后骨组织表面超微结构的变化

发布时间：2018-10-21 17:29

【摘要】：目的观察实验性大鼠骨质疏松模型股骨颈超微结构的变化特点,并探讨利用原子力显微镜观察的可行性。方法清洁级雌性SD大鼠(鼠龄3个月)60只,体重(200±20)g。随机将其分成对照组(Sham组)、去卵巢组(OVX组)、阿伦膦酸钠治疗组(ALN组)、PEMFs治疗组(PEMFs组),每组大鼠15只。除Sham组外,对其他各组行双侧卵巢切除术,造模后第30天开始分组干预。ALN组给予阿伦膦酸钠灌胃,PEMFs组给予脉冲电磁场治疗。Sham组和OVX组手术后正常喂养,不予任何处理。治疗后30d取大鼠股骨头切片,通过AFM观察大鼠股骨颈的表面超微结构。结果 Sham组原子力显微镜扫描可显示骨组织的骨陷窝、骨小管及其表面的钙磷晶体沉积,骨组织表面粗糙度为(2.59±0.645)μm。OVX组可见骨陷窝的大小变化及其钙磷晶体的排列紊乱,模型组骨组织表面粗糙度明显高于对照组(P0.01),提示造模成功;ALN组表面粗糙度明显低于OVX组(P0.05),PEMFs组的表面粗糙度与OVX组相比,有明显降低,差异具有统计学意义(P0.05);而PEMFs组与ALN组比较,其表面粗糙度无明显差异(P0.05)。结论通过原子力显微镜能清晰观察到脉冲电磁场对大鼠骨质疏松治疗后的骨组织表面超微结构的变化,其疗效与阿仑膦酸钠相当,为脉冲电磁场治疗骨质疏松提供了一定的理论依据。
[Abstract]:Objective to observe the ultrastructural changes of femoral neck in experimental osteoporosis rats and to explore the feasibility of atomic force microscopy (AFM). Methods Sixty clean grade female SD rats (aged 3 months) with body weight of (200 卤20) g were used. They were randomly divided into control group (Sham group), ovariectomized group (OVX group) and alendronate treatment group (ALN group), PEMFs treatment group (PEMFs group), 15 rats in each group. In addition to Sham group, bilateral ovariectomy was performed in other groups. The rats in ALN group were given alendronate intragastric perfusion on the 30th day after modeling. The PEMFs group was treated with pulsed electromagnetic field. The Sham group and OVX group were fed normally after operation without any treatment. The surface ultrastructure of femoral neck of rats was observed by AFM 30 days after treatment. Results in the Sham group, the bone lacunae, the calcium and phosphorus crystals on the bone tubules and their surfaces were found to be deposited by atomic force microscopy. The bone surface roughness was (2.59 卤0.645) 渭 m.OVX. The size of the bone lacunae and the disarrangement of calcium and phosphorus crystals were found in the bone tissue of the group of (2.59 卤0.645) 渭 m.OVX. The surface roughness of bone tissue in the model group was significantly higher than that in the control group (P0.01), suggesting that the surface roughness of the ALN group was significantly lower than that of the OVX group (P0.05) compared with the OVX group (P0.05), while the surface roughness of the PEMFs group was significantly lower than that of the ALN group (P0.05). There was no significant difference in surface roughness (P0.05). Conclusion the ultrastructural changes of bone surface after treatment of osteoporosis in rats can be clearly observed by atomic force microscope. The effect of pulsed electromagnetic field is similar to that of alendronate. It provides a theoretical basis for the treatment of osteoporosis by pulsed electromagnetic field.
【作者单位】：重庆医科大学附属康复医院;
【分类号】：R580

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