羊膜间充质干细胞移植改善百草枯中毒肺纤维化大鼠肺组织病理学变化

发布时间：2018-10-23 09:46

【摘要】：目的探讨人羊膜间充质干细胞(hAMSCs)移植对百草枯中毒肺纤维化大鼠模型肺组织病理学变化的影响。方法将46只SD大鼠随机分为假手术组、模型组和hAMSCs移植组。2%百草枯灌胃[100 mg/(kg·只)]制备肺纤维化模型,尾静脉移植hAMSCs[2×10~6个细胞/(mL·只)],HE和免疫组化染色后,显微镜下观察肺组织病理学变化。结果与模型组比较,hAMSCs移植组大鼠一般状况较好。肉眼观发现,模型组大鼠两肺叶出现较多大面积白色纤维化病灶,hAMSCs移植组则为较少、散在的点片状病灶。显微镜下可见,模型组大鼠肺组织结构紊乱,肺泡壁增厚,有大面积间质增生和大量炎细胞浸润,而hAMSCs移植组肺组织结构明显改善,肺泡壁多为单层细胞,仅有少量、小面积间质增生,炎细胞浸润显著地减少,且有较多抗人细胞核特异性抗体阳性hAMSCs定植、存活于肺间质,少见于肺泡壁。结论移植的hAMSCs可定植、存活于肺组织,发挥治疗百草枯中毒肺纤维化的作用。
[Abstract]:Objective to investigate the effect of human amniotic mesenchymal stem cell (hAMSCs) transplantation on lung histopathological changes in paraquat induced pulmonary fibrosis rats. Methods 46 SD rats were randomly divided into sham-operation group, model group and hAMSCs transplantation group. 2% paraquat was perfused into the stomach [100 mg/ (kg)] to make pulmonary fibrosis model. HAMSCs was transplanted into tail vein [2 脳 106 cells / (mL)], HE and immunohistochemical staining were used. The histopathological changes of lung were observed under microscope. Results compared with the model group, the general condition of the hAMSCs transplantation group was better. In the naked eye, there were more white fibrosis lesions in the two lobes of the model group than in the hAMSCs transplantation group. In the model group, the pulmonary tissue structure was disorder, the alveolar wall was thickened, the interstitial hyperplasia and inflammatory cell infiltration were found in large area, but the lung tissue structure was improved obviously in the hAMSCs transplantation group, the alveolar wall was mostly monolayer cells, only a few of them were found in the model group. Small areas of interstitial hyperplasia, inflammatory cell infiltration significantly decreased, and more anti-human nuclear specific antibody positive hAMSCs colonization, survival in the interstitial lung, rarely in the alveolar wall. Conclusion the transplanted hAMSCs can survive in lung tissue and play a role in the treatment of pulmonary fibrosis caused by paraquat poisoning.
【作者单位】：遵义医学院附属医院/贵州省细胞工程重点实验室/贵州省生物治疗人才基地;遵义医学院教育部基础药理重点实验室;
【基金】：国家自然科学基金项目(编号:81260507) 遵义市干细胞与再生医学工程研究中心建设项目[编号:省市科合(2014)59号]
【分类号】：R595.4

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