条件性敲除胰岛β细胞蛋白磷酸酶2Acα对小鼠糖代谢影响机制的探讨

发布时间：2018-10-31 07:55

【摘要】：目的探讨条件性敲除胰岛β细胞蛋白磷酸酶2Acα(PP2Acα)对小鼠血糖水平影响的机制。方法选取4月龄条件性敲除胰岛β细胞PP2Acα(KO组)及对照组(Con,与KO组同窝别、性别匹配)各24只。行腹腔葡萄糖耐量实验(IPGTT),测定0、30、60和120min小鼠血糖及胰岛素分泌水平;新鲜分离胰岛,用葡萄糖刺激胰岛素分泌实验(GSIS)评价小鼠胰岛β细胞对葡萄糖反应性。结果IPGTT结果显示,KO组葡萄糖负荷后30min血糖[(23.1±5.1)vs(17.5±5.7)mmol/L]和60min血糖[(20.5±6.8)vs(13.5±5.1)mmol/L]高于Con组(P0.05);KO组葡萄糖负荷后30min血清胰岛素水平较Con组下降[(2.16±0.92)vs(1.07±0.42)μg/L](P0.05);GSIS显示高糖刺激下KO组胰岛素分泌较Con组减少[(0.82±0.15)vs(0.42±0.24)μIU/L](P0.05)。结论条件性失活胰岛β细胞PP2Acα小鼠糖耐量受损可能与胰岛素一相分泌受损相关。
[Abstract]:Objective to investigate the effect of conditioned knockout 尾 -cell protein phosphatase 2Ac 伪 (PP2Ac 伪) on blood glucose level in mice. Methods four months old 尾 -cell PP2Ac 伪 (KO group) and control group (Con, and KO group) were randomly divided into two groups: Con, group and KO group. Glucose tolerance test (IPGTT),) was performed to determine the levels of blood glucose and insulin secretion in mice with 30 ~ (60) and 120min, and the islets were isolated freshly, and the islet 尾 -cells were evaluated by glucose stimulated insulin secretion test (GSIS) to evaluate the responsiveness of islet 尾 cells to glucose. Results IPGTT results showed that the blood glucose of 30min [(23.1 卤5.1) vs (17.5 卤5.7) mmol/L] and 60min (20.5 卤6.8) vs (13.5 卤5.1) mmol/L in KO group were higher than those in Con group (P0.05). The serum insulin level of 30min in KO group was lower than that in Con group [(2.16 卤0.92) vs (1.07 卤0.42) 渭 g / L] (P0.05). GSIS showed that insulin secretion in KO group was lower than that in Con group [(0.82 卤0.15) vs (0.42 卤0.24) 渭 IU/L] (P0.05). Conclusion impaired glucose tolerance in conditioned inactivated islet 尾-cell PP2Ac 伪 mice may be associated with impaired insulin-phase secretion.
【作者单位】：南京医科大学附属南京医院(南京市第一医院)内分泌科;徐州市第一人民医院内分泌科;
【基金】：中国博士后科学基金(2015M581829)
【分类号】：R587.1

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