免疫分子B7-H3在类风湿性关节炎外周血单核细胞上的高表达及其临床意义

发布时间：2018-10-31 18:54

【摘要】：目的分析类风湿性关节炎外周血单核细胞上B7-H3分子的表达及其与疾病活动程度、炎症因子水平间的相关性。方法收集69例类风湿性关节炎患者及38例健康对照组外周血,Ficoll分离单个核细胞后流式细胞仪检测CD14、B7-H3分子的表达,ELISA检测炎症因子TNF-α、IL-6的表达。统计学分析其相关性及与疾病活动程度之间的关联。结果类风关患者外周血单核细胞上B7-H3表达较健康对照组高(P0.001),且与疾病的分期、病程的长短、肿胀关节数、压痛关节数、疾病活动度评分28(DSA28)、类风湿因子有显著相关性,并与炎症因子TNF-α、IL-6呈正相关(P0.001)。结论免疫分子B7-H3可能参与类风湿性关节炎疾病的进程并可能成为疾病预防及治疗的潜在分子。
[Abstract]:Objective to investigate the expression of B7-H3 in peripheral blood monocytes of rheumatoid arthritis (RA) and its correlation with disease activity and inflammatory cytokines. Methods in 69 patients with rheumatoid arthritis and 38 healthy controls, the expression of CD14,B7-H3 molecule was detected by flow cytometry after mononuclear cells were isolated by Ficoll, and the expression of TNF- 伪 and IL-6 was detected by ELISA. The correlation and association with disease activity were analyzed statistically. Results the expression of B7-H3 on peripheral blood monocytes in patients with Fengguan was higher than that in healthy controls (P0. 001), and was correlated with the stages of disease, the duration of disease, the number of swollen joints, the number of tenderness joints, and the disease activity score 28 (DSA28). There was a significant correlation between rheumatoid factors and inflammatory factors TNF- 伪 and IL-6 (P0. 001). Conclusion immune molecule B7-H3 may be involved in the process of rheumatoid arthritis and may be a potential molecule in the prevention and treatment of rheumatoid arthritis.
【作者单位】：苏州卫生职业技术学院医学技术学院;无锡市红十字中心血站;苏州大学附属第一医院临床免疫研究所;
【基金】：国家自然科学基金(31170836) 江苏省高校优秀科技创新团队项目(2015023) 江苏省“青蓝工程”中青年学术带头人项目(2014)
【分类号】：R593.22

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