SD大鼠极端高温高湿环境暴露的实验研究

发布时间：2018-11-03 17:42

【摘要】：目的调查极端高温高湿环境暴露对SD大鼠生存、血液生化指标的影响及对主要脏器的伤害。方法 26只SD大鼠分为常温常湿组和极端高温高湿组。观察SD大鼠在极端高温高湿环境中的死亡情况并于半数致死时间点捕杀动物进行血清生化指标测定和心、肺、肾、脑、肌肉和小肠6种组织的病理检测。结果极端高温高湿环境暴露可导致SD大鼠死亡,半数致死时间为48h;极端高温高湿组与常温常湿组相比,SD大鼠血清肌酐(Scr)显著性升高(P0.05),血清碱性磷酸酶(ALP)、缺血修饰性清蛋白(IMA)明显下降(P0.05),其他生化指标无显著性改变;极端高温高湿组SD大鼠心、肺、肾、小肠组织可见炎性细胞浸润,脑和肌肉组织未见明显病理性改变。结论极端高温高湿环境暴露对SD大鼠有明显伤害,可引起某些血液生化指标异常和心、肺、肾、小肠组织的炎性改变,甚至造成死亡。
[Abstract]:Objective to investigate the effects of extreme high temperature and high humidity exposure on survival, blood biochemical indexes and injury to main organs of SD rats. Methods 26 SD rats were divided into normal temperature and normal humidity group and extreme high temperature and high humidity group. To observe the death of SD rats in extremely high temperature and high humidity environment, and to determine the biochemical indexes of serum and the pathological examination of heart, lung, kidney, brain, muscle and small intestine at half lethal time. Results exposure to extreme high temperature and high humidity resulted in the death of SD rats, and the median lethal time was 48 hours. Compared with normal temperature and humidity group, serum creatinine (Scr) in SD rats was significantly increased (P0.05), serum alkaline phosphatase (ALP), ischemic modified albumin (IMA) was significantly decreased (P0.05). There was no significant change in other biochemical indexes. Inflammatory cell infiltration was observed in heart, lung, kidney and small intestine of SD rats in extremely high temperature and high humidity group, but no pathological changes were found in brain and muscle tissues. Conclusion exposure to extreme high temperature and high humidity can cause obvious injury to SD rats, which can cause some blood biochemical changes and inflammatory changes in heart, lung, kidney and small intestine, and even cause death.
【作者单位】：西南交通大学生命科学与工程学院;中国人民解放军成都军区总医院转化医学科/高湿医学全军重点实验室;中国人民解放军成都军区总医院病理科;中国人民解放军成都军区总医院中医科;
【基金】：四川省科技厅青年科技创新研究团队资助(2013TD0016)
【分类号】：R594

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