白藜芦醇调节抗氧化酶活性预防去卵巢大鼠骨质疏松的研究

发布时间：2018-11-09 19:03

【摘要】：[目的]探讨白藜芦醇(RES)通过调节抗氧化酶活性对去卵巢大鼠骨质疏松的预防作用。[方法]6月龄健康雌性SD大鼠30只按照随机对照分组原则分为5组,每组6只,分别设为假手术组(SHAM),单纯去卵巢组(OVX),低剂量白藜芦醇组(RL),中剂量白藜芦醇组(RM)，，峋,高剂量白藜芦醇组(RH)。各组大鼠经过手术切除双侧卵巢建立绝经后骨质疏松模型(除假手术组),1周后给予不同剂量的RES预防性治疗,每周称量体重。连续干预12周后采血检测血清相关生化指标,处死后迅速获取股骨、子宫标本,称量子宫湿重。采用双能X线吸收法测定大鼠的股骨骨密度,采用HE染色法光镜下观察股骨组织形态学变化,通过逆转录RCR (RT-PCR)检测股骨组织中γ谷氨酰半胱氨酸合成酶重亚单位(y-GCSh)、血红素加氧酶1(HO-1)和过氧化氢酶(CAT)的mRNA表达情况。[结果]1与SHAM组比较,OVX组血清生化指标：钙(Cα2+)、磷(IP)、碱性磷酸酶(ALP)和抗酒石酸酸性磷酸酶5b(TRACP-5b)显著升高,雌激素水平明显下降,子宫湿重减少,股骨骨密度明显降低,股骨组织HE染色显示骨组织呈现骨质疏松状况,抗氧化酶：γ谷氨酰半胱氨酸合成酶重亚单位(γ-GCSh)、血红素加氧酶1(HO-1)和过氧化氢酶(CAT)的mRNA表达均明显下降。2.与OVX组比较,RES不同剂量治疗组血清生化指标：Ca2+、IP、ALP和TRACP-5b显著降低,雌激素水平明显增加,子宫湿重增加,股骨骨密度明显升高,股骨组织HE染色呈现改善后的骨质疏松状况,抗氧化酶：y-GCSh、HO-1和CAT的mRNA表达均上调。[结论]1.RES对去卵巢大鼠的子宫萎缩有一定的抑制作用。2.RES能有效的降低血清中升高的ALP、TRACP-5b、Ca2+和IP,改善了去卵巢大鼠的高转换状态。3.RES在20-40mg/(kg·d)剂量范围内均能有效抑制去卵巢大鼠的骨丢失,改善去卵巢大鼠的骨密度,其机理可能与其具有雌激素样活性有关。4.RES可以提高去卵巢大鼠骨组织中抗氧化酶γ-GCS、HO-1和CAT的活性,改善氧化应激状态,调节骨代谢平衡,对去卵巢大鼠骨质疏松有一定的预防作用。
[Abstract]:[objective] to investigate the preventive effect of resveratrol (RES) on osteoporosis in ovariectomized rats by regulating the activity of antioxidant enzymes. [methods] Thirty 6-month-old healthy female SD rats were divided into 5 groups, 6 in each group, according to the principle of randomized control. They were divided into sham-operation group, (SHAM), group, simple ovariectomized group, (OVX), low-dose resveratrol group, (RL), group. Middle dose resveratrol group (RM), high dose resveratrol group (RH). The model of postmenopausal osteoporosis was established by resection of bilateral ovaries in each group (except sham-operation group). One week later, the rats were given different doses of RES prophylactic therapy, weighing their body weight every week. Serum biochemical indexes were measured after 12 weeks of continuous intervention. Femur and uterus samples were obtained quickly after death and uterine wet weight was measured. Bone mineral density (BMD) of femur in rats was measured by dual energy X-ray absorptiometry. The morphological changes of femur were observed under light microscope with HE staining. The expression of 纬 -glutamyl cysteine synthase heavy subunit (y-GCSh), heme oxygenase-1 (HO-1) and catalase (CAT) (CAT) in femur was detected by reverse transcription RCR (RT-PCR). [results] 1Compared with SHAM group, serum biochemical indexes of OVX group: calcium (C 伪 2), phosphorous (IP), alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase 5b (TRACP-5b), estrogen level significantly decreased, uterus wet weight decreased. Bone mineral density of femur decreased obviously, HE staining of femur tissue showed osteoporosis, antioxidant enzyme: 纬-glutamyl cysteine synthase heavy subunit (纬-GCSh),) The mRNA expression of heme oxygenase 1 (HO-1) and catalase (CAT) decreased significantly. Compared with the OVX group, the serum biochemical indexes of different doses of RES: Ca2, IP,ALP, TRACP-5b, estrogen level, uterine wet weight, femur bone mineral density were significantly decreased, estrogen level was significantly increased, and femur bone mineral density was significantly increased. The HE staining of femur showed improved osteoporosis, and the expression of antioxidant enzyme: y-GCShOHO-1 and CAT were all up-regulated. [conclusion] 1.RES can inhibit uterine atrophy in ovariectomized rats to some extent. 2.RES can effectively reduce elevated ALP,TRACP-5b,Ca2 and IP, in serum. 3.RES could effectively inhibit bone loss in ovariectomized rats and improve bone density in ovariectomized rats. The mechanism may be related to its estrogen-like activity. 4.RES can increase the activities of antioxidant enzymes 纬-GCS,HO-1 and CAT in bone tissue of ovariectomized rats, improve the state of oxidative stress and regulate the balance of bone metabolism. It can prevent osteoporosis in ovariectomized rats.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R580;R683

【相似文献】