类风湿关节炎患者关节滑膜滑液中结缔组织生长因子表达及其意义研究

发布时间：2018-11-14 09:32

【摘要】：目的检测结缔组织生长因子(connectivetissue growth factor,CTGF)在类风湿关节炎(rheumatoid arthritis,RA)患者关节滑液及滑膜中的表达,探索CTGF在RA关节病变中可能发挥的作用。方法采用酶联免疫法(enzyme-linked imm-unosorbent assay,ELISA)方法检测中国医科大学附属第一医院2013年1月至12月收治的10例RA患者和8例骨关节炎(osteoar thritis,OA)患者关节滑液CTGF水平;免疫组化法检测3例RA患者和2例OA患者关节滑膜CTGF的表达;Real-time PCR检测向体外培养的成纤维样滑膜细胞(FLS)中加入外源性肿瘤坏死因子α(tumor necrosis factor alpha,TNF-α)后对CTGF表达的影响。结果 RA组的关节滑液CTGF水平高于OA组[(21.00±10.20)μg/L对(8.53±7.71)μg/L)],差异有统计学意义(P0.05);CTGF在RA滑膜组织主要表达于滑膜衬里细胞及衬里下层细胞,而OA滑膜组织仅少量CTGF表达;TNF-α可以上调FLS表达CTGF。结论 RA患者滑液CTGF水平升高,滑膜组织CTGF高表达,提示CTGF可能参与了RA关节炎症的发生发展。TNF-α上调FLS的CTGF表达,这可能是导致RA关节病变的重要因素之一。
[Abstract]:Objective to detect the expression of connective tissue growth factor (connectivetissue growth factor,CTGF) in synovial fluid and synovium of patients with rheumatoid arthritis (rheumatoid arthritis,RA) and to explore the role of CTGF in RA joint lesions. Methods the levels of CTGF in synovial fluid of 10 patients with RA and 8 patients with osteoarthritis (osteoar thritis,OA) were detected by enzyme linked immunosorbent assay (enzyme-linked imm-unosorbent assay,ELISA) from January to December 2013 in the first affiliated Hospital of China Medical University. The expression of CTGF in synovial membrane of 3 patients with RA and 2 patients with OA was detected by immunohistochemistry. Real-time PCR was used to detect the effect of exogenous tumor necrosis factor 伪 (tumor necrosis factor alpha,TNF- 伪 (TNF- 伪) on the expression of CTGF in cultured fibroblast (FLS). Results the level of CTGF in synovial fluid in RA group was significantly higher than that in OA group [(21.00 卤10.20) 渭 g / L vs (8.53 卤7.71) 渭 g / L] (P0.05). CTGF was mainly expressed in synovial lining cells and sublining cells of RA, but only a small amount of CTGF was expressed in OA synovium, and TNF- 伪 could up-regulate FLS expression of CTGF.. Conclusion the increase of CTGF level in synovial fluid and the high expression of CTGF in synovial tissue in patients with RA suggest that CTGF may be involved in the development of RA arthritis. TNF- 伪 upregulated CTGF expression of FLS, which may be one of the important factors leading to RA joint lesion.
【作者单位】：中国医科大学附属第一医院风湿免疫科;
【基金】：辽宁省医学高峰建设工程专项经费项目任务实施方案(2010006)
【分类号】：R593.22

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