Toll样受体4与IL-7和IL-10在干燥综合征患者唇腺组织中表达及其意义研究
发布时间：2018-11-14 16:58
【摘要】：目的:了解干燥综合征患者唇腺组织内TLR4与IL-7和IL-10的表达情况,并分析各因子与其它实验室指标之间的相关性,探索TLR4及其相关细胞因子在干燥综合征发生、发展过程中的作用,为干燥综合征的临床诊断与治疗提供新思路。方法:.以蚌埠医学院第一附属医院于2010年2月至2013年10月间风湿免疫科收治的74例干燥综合征患者为研究组,以同期收治的32例非干燥综合征患者为对照组,获取所有患者的唇腺病理切片采用Elivision免疫组化染色方法检测、同时采用人工计数方法进行染色结果的定性和半定量分析,进行如下研究:(1)分析研究组和对照组患者唇腺内的TLR4、IL-7、IL-10表达阳性率、阳性细胞染色积分和显色强度。(2)分析TLR4、IL-7、IL-10三种因子阳性表达之间的相关性,探讨TLR4、IL-7、IL-10与其它实验室指标之间的相关性。(3)对比原发性和继发性干燥综合征患者及对照组唇腺内TLR4、IL-7、IL-10的阳性表达情况有无差异,并探讨其意义。最后借助于SPSS13.0软件包进行统计学分析。计量数据以(±s)表示,组间对比行t检验。计数据以n(%)表示,组间对比行x2检查。多组间对比行AVONA中的SNK-q检验。相关性分析采用Spearman相关分析。组间检验水平a=0.05,依据组数进行修正。以P0.05表示差异具有统计学意义,以P0.01表示组间差异具有显著统计学意义。结果:(1)TLR4在研究组患者唇腺内的阳性表达率为79.72%,对照组为53.12%,差异具有统计学意义(x2=5.471,P=0.031)。研究组患者的TLR4阳性细胞显色程度在实验组为1.27±0.29,在对照组中为0.46±0.51,P=0.013;研究组TLR4阳性细胞染色积分在为3.24±3.07,在对照组为0.58±0.66,P0.001且差异具有统计学意义。(2)研究组患者唇腺内IL-7的阳性表达率为85.14%,对照组为21.87%,差异具有统计学意义(x2=17.539,P=0.000)。研究组IL-7阳性细胞显色程度为1.31±0.27,在对照组中为0.26±0.11,P=0.021;研究组IL-7阳性细胞染色积分为3.37±1.61,在对照组为0.54±0.67,P=0.014;且差异具有统计学意义。(3)研究组患者唇腺内IL-10的阳性表达率为82.43%,对照组为34.28%,两组间差异具有统计学意义(x2=10.204,P=0.013)。研究组IL-10的阳性细胞显色程度为1.49±0.22,在对照组为0.31±0.17,P=0.019,研究组IL-10阳性细胞染色积分为3.41±1.04,在对照组为0.59±0.61,P=0.024,且差异具有统计学意义。(4)TLR4、IL-7、IL-10三种因子的阳性表达率间均相互呈正相关,且具有统计学意义(P0.05)。(5)原发性干燥综合征与继发性干燥综合征患者唇腺内的TLR4、IL-7、IL-10阳性表达率均明显高于对照组,差异具有统计学意义(P0.05)。但原发性和继发性干燥综合征患者腺体内的TLR4、IL-7、IL-10阳性表达情况无统计学差异(P0.05)。(6)IL-7和IL-10的阳性表达与ESR呈正相关(r=0.539,P=0.003;r=0.494,P=0.013)。TLR4的阳性表达则分别与Ig M(r=0.358,P=0.03),Ig A(r=0.461,P=0.012)、ESR(r=0.303,P=0.032)等免疫指标或炎性指标呈正相关,差异具有统计学意义(P0.05)。结论:(1)干燥综合征患者唇腺内的TLR4与IL-7和IL-10的表达水平均明显高于正常对照组,但原发性和继发性患者之间无显著差异,提示TLR4信号转导途径及其相关细胞因子可能参与干燥综合征发病过程;(2)TLR4与IL7、IL10的表达水平与ESR及血清Ig M、Ig A的表达呈正相关,提示TLR4与IL7、IL10的表达水平与病情活动有一定的相关性,可作为临床判断病情活动度的参考指标;(3)TLR4与IL-7、IL-10的表达与多种炎性或免疫因子可能有一定的相关性,针对TLR4信号导途径的靶向治疗可能有利于控制干燥综合征病情的发展。
[Abstract]:Objective: To study the expression of TLR4 and IL-7 and IL-10 in the lip gland of patients with dry syndrome, and to analyze the correlation between the factors and other laboratory indexes, and to explore the role of TLR4 and its associated cytokines in the pathogenesis and development of dry syndrome. and provides a new thought for clinical diagnosis and treatment of the dry syndrome. Method:. In the first Affiliated Hospital of Bengbu Medical College from February 2010 to October 2013, 74 patients with dry syndrome were treated as the study group, and 32 patients with non-dry syndrome treated in the same period were the control group. The positive rate of TLR4, IL-7 and IL-10 in the labial gland of the study group and the control group was analyzed. The staining and developing intensity of positive cells. (2) The correlation between the three factors of TLR4, IL-7 and IL-10 was analyzed, and the correlation between TLR4, IL-7, IL-10 and other laboratory indexes was discussed. (3) The positive expression of TLR4, IL-7 and IL-10 in the lip glands of patients with primary and secondary drying syndrome and the control group were different, and their significance was discussed. Finally, the statistical analysis was performed with the help of the SPSS13.0 software package. The measurement data is expressed as (% s), and the inter-group comparison line t is tested. The count data is represented by n (%), and the inter-group comparison line x2 is checked. The SNK-q test in a multi-group comparison line, AVONA. The correlation analysis was analyzed by Spearman. The level of test between the groups was a = 0.05, and the group number was corrected. The difference between the groups was statistically significant with the difference of P0.05, and the difference between the groups was statistically significant. Results: (1) The positive expression rate of TLR4 in the labial gland of the study group was 72.72%, the control group was 53.12%, and the difference was of statistical significance (x2 = 5.471, P = 0.031). The color of TLR4 positive cells in the study group was 1.27 to 0.29 in the experimental group and 0.46 to 0.51, P = 0.013 in the control group. The staining and integration of TLR4 positive cells in the study group were 3.24 and 3.07, and 0.58, 0.66, P0.001 in the control group, and the difference was of statistical significance. (2) The positive expression of IL-7 in the lip glands of the study group was 85. 14%, the control group was 21. 87%, and the difference was statistically significant (x2 = 175.539, P = 0.000). The level of IL-7 positive cells in the study group was 1.31 and 0.27. In the control group, the staining score of IL-7 positive cells was 0.26, 0.11, P = 0.021, and that of IL-7 positive cells in the study group was 3.37-1.61, and the control group was 0.54-0.67, P = 0.014; and the difference was of statistical significance. (3) The positive expression rate of IL-10 in the labial gland of the study group was 82.43%, the control group was 34. 28%, and the difference between the two groups was statistically significant (x2 = 10.204, P = 0.013). The staining degree of IL-10 positive cells in the study group was 1.49 and 0.22. In the control group, the staining score of IL-10 positive cells was 3.41-1.04, the control group was 0.59-0.61, P = 0.024, and the difference was of statistical significance. (4) The positive expression rates of TLR4, IL-7 and IL-10 were positively correlated with each other and were of statistical significance (P0.05). (5) The positive rate of TLR4, IL-7 and IL-10 in the lip glands of patients with primary and secondary drying syndrome was significantly higher than that in the control group (P0.05). The expression of TLR4, IL-7 and IL-10 in the gland of patients with primary and secondary drying syndrome was not statistically different (P0.05). (6) The positive expression of IL-7 and IL-10 was positively correlated with ESR (r = 0.539, P = 0.003; r = 0.494, P = 0.013). The positive expression of TLR4 was positively correlated with that of Ig M (r = 0.358, P = 0.03), Ig A (r = 0.461, P = 0.012), and ESR (r = 0.303, P = 0.032). Conclusion: (1) The expression level of TLR4 and IL-7 and IL-10 in the lip gland of the patients with dry syndrome is significantly higher than that in the normal control group, but there is no significant difference between the primary and secondary patients, suggesting that the TLR4 signal transduction pathway and its associated cytokines may be involved in the pathogenesis of the dry syndrome; (2) The expression level of TLR4 and IL7 and IL10 is positively correlated with the expression of the ESR and the serum Ig M and Ig A, suggesting that the expression level of TLR4 and IL7 and IL10 has a certain correlation with the activity of the disease, and can be used as a reference index for clinical judgment of the disease activity; (3) TLR4 and IL-7, The expression of IL-10 may be related to various inflammatory or immune factors, and the targeted therapy for TLR4 signaling pathway may be beneficial to the control of the development of dry syndrome.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R593.2
本文编号：2331763
[Abstract]:Objective: To study the expression of TLR4 and IL-7 and IL-10 in the lip gland of patients with dry syndrome, and to analyze the correlation between the factors and other laboratory indexes, and to explore the role of TLR4 and its associated cytokines in the pathogenesis and development of dry syndrome. and provides a new thought for clinical diagnosis and treatment of the dry syndrome. Method:. In the first Affiliated Hospital of Bengbu Medical College from February 2010 to October 2013, 74 patients with dry syndrome were treated as the study group, and 32 patients with non-dry syndrome treated in the same period were the control group. The positive rate of TLR4, IL-7 and IL-10 in the labial gland of the study group and the control group was analyzed. The staining and developing intensity of positive cells. (2) The correlation between the three factors of TLR4, IL-7 and IL-10 was analyzed, and the correlation between TLR4, IL-7, IL-10 and other laboratory indexes was discussed. (3) The positive expression of TLR4, IL-7 and IL-10 in the lip glands of patients with primary and secondary drying syndrome and the control group were different, and their significance was discussed. Finally, the statistical analysis was performed with the help of the SPSS13.0 software package. The measurement data is expressed as (% s), and the inter-group comparison line t is tested. The count data is represented by n (%), and the inter-group comparison line x2 is checked. The SNK-q test in a multi-group comparison line, AVONA. The correlation analysis was analyzed by Spearman. The level of test between the groups was a = 0.05, and the group number was corrected. The difference between the groups was statistically significant with the difference of P0.05, and the difference between the groups was statistically significant. Results: (1) The positive expression rate of TLR4 in the labial gland of the study group was 72.72%, the control group was 53.12%, and the difference was of statistical significance (x2 = 5.471, P = 0.031). The color of TLR4 positive cells in the study group was 1.27 to 0.29 in the experimental group and 0.46 to 0.51, P = 0.013 in the control group. The staining and integration of TLR4 positive cells in the study group were 3.24 and 3.07, and 0.58, 0.66, P0.001 in the control group, and the difference was of statistical significance. (2) The positive expression of IL-7 in the lip glands of the study group was 85. 14%, the control group was 21. 87%, and the difference was statistically significant (x2 = 175.539, P = 0.000). The level of IL-7 positive cells in the study group was 1.31 and 0.27. In the control group, the staining score of IL-7 positive cells was 0.26, 0.11, P = 0.021, and that of IL-7 positive cells in the study group was 3.37-1.61, and the control group was 0.54-0.67, P = 0.014; and the difference was of statistical significance. (3) The positive expression rate of IL-10 in the labial gland of the study group was 82.43%, the control group was 34. 28%, and the difference between the two groups was statistically significant (x2 = 10.204, P = 0.013). The staining degree of IL-10 positive cells in the study group was 1.49 and 0.22. In the control group, the staining score of IL-10 positive cells was 3.41-1.04, the control group was 0.59-0.61, P = 0.024, and the difference was of statistical significance. (4) The positive expression rates of TLR4, IL-7 and IL-10 were positively correlated with each other and were of statistical significance (P0.05). (5) The positive rate of TLR4, IL-7 and IL-10 in the lip glands of patients with primary and secondary drying syndrome was significantly higher than that in the control group (P0.05). The expression of TLR4, IL-7 and IL-10 in the gland of patients with primary and secondary drying syndrome was not statistically different (P0.05). (6) The positive expression of IL-7 and IL-10 was positively correlated with ESR (r = 0.539, P = 0.003; r = 0.494, P = 0.013). The positive expression of TLR4 was positively correlated with that of Ig M (r = 0.358, P = 0.03), Ig A (r = 0.461, P = 0.012), and ESR (r = 0.303, P = 0.032). Conclusion: (1) The expression level of TLR4 and IL-7 and IL-10 in the lip gland of the patients with dry syndrome is significantly higher than that in the normal control group, but there is no significant difference between the primary and secondary patients, suggesting that the TLR4 signal transduction pathway and its associated cytokines may be involved in the pathogenesis of the dry syndrome; (2) The expression level of TLR4 and IL7 and IL10 is positively correlated with the expression of the ESR and the serum Ig M and Ig A, suggesting that the expression level of TLR4 and IL7 and IL10 has a certain correlation with the activity of the disease, and can be used as a reference index for clinical judgment of the disease activity; (3) TLR4 and IL-7, The expression of IL-10 may be related to various inflammatory or immune factors, and the targeted therapy for TLR4 signaling pathway may be beneficial to the control of the development of dry syndrome.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R593.2
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1 赖蓓,陈巧林,张翠华,王振刚 ,栗占国;免疫印迹法检测抗胆碱能毒蕈碱受体3抗体及其在干燥综合征诊断中的意义[J];中华风湿病学杂志;2005年07期
2 李丽燕;;Toll样受体的研究进展[J];临床医学工程;2011年03期
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