重组Furin蛋白对类风湿关节炎滑膜细胞生物学行为的影响

发布时间：2018-11-16 17:17

【摘要】：[目的]探讨重组弗林蛋白(Furin)对于类风湿性关节炎(rheumatoid arthritis,RA)滑膜细胞增殖、迁移、侵袭和细胞因子分泌的影响。[方法]从类风湿关节炎患者关节内提取RA滑膜组织后培养原代滑膜成纤维细胞(fibroblast-like synovial cells,FLS);将重组Furin蛋白按照不同浓度(250 ng/ml,500 ng/ml)加入RA滑膜细胞培养基中诱导培养并采用MTT、细胞划痕、Transwell、ELISA等方法检测重组蛋白处理对细胞增殖、迁移、侵袭和炎性因子分泌等生物学特性的影响。[结果]与空白对照组比,加入重组Furin蛋白处理24 h、48 h,对类风湿滑膜细胞增殖活动没有明显影响(P0.05)。处理24 h后与空白对照组比,迁入划痕伤口内细胞数无显著区别(P0.05),滑膜细胞穿透基底膜细胞数明显减少(P0.05),且与剂量浓度呈正相关。培养液上清中IL-1α和IL-17含量升高(P0.05),而各组间IL-1β和TNF-α含量差异无统计学意义(P0.05)。[结论]重组Furin蛋白诱导可抑制类风湿关节炎滑膜细胞侵袭能力同时可促进其分泌IL-1α和IL-17。
[Abstract]:[objective] to investigate the effects of recombinant Flynn (Furin) on proliferation, migration, invasion and cytokine secretion of synovial cells in patients with rheumatoid arthritis (rheumatoid arthritis,RA). [methods] the primary synovial fibroblasts (fibroblast-like synovial cells,FLS) were isolated from the synovial tissue of patients with rheumatoid arthritis (RA). The recombinant Furin protein was induced and cultured in RA synovial cell culture medium with different concentrations (250 ng/ml,500 ng/ml), and MTT, cell scratch was used to detect the proliferation and migration of the cells treated with recombinant Furin protein by Transwell,ELISA. The effects of biological characteristics such as invasion and inflammatory factor secretion. [results] compared with the control group, the proliferation of rheumatoid synovial cells was not significantly affected by adding recombinant Furin protein for 24 h or 48 h (P0.05). Compared with the control group 24 hours after treatment, the number of cells in the wound moved into the scratch wound had no significant difference (P0.05), and the number of the synovial cells penetrating through the basement membrane decreased significantly (P0.05), which was positively correlated with the concentration of the dose. The content of IL-1 伪 and IL-17 in the supernatant of culture medium was increased (P0.05), but the content of IL-1 尾 and TNF- 伪 was not significantly different among the groups (P0.05). [conclusion] Recombinant Furin protein can inhibit the invasiveness of synovial cells in rheumatoid arthritis and promote the secretion of IL-1 伪 and IL-17..
【作者单位】：山东大学附属省立医院关节外科;
【基金】：山东省卫生厅发展规划项目(编号:2001BB1DBA3)
【分类号】：R593.22

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