二维相关光谱分析在无创血糖检测特异性研究中的应用

发布时间：2018-11-18 12:03

【摘要】：主要利用二维相关光谱分析方法考察了血液中主要成分、测量过程中的系统漂移等因素对近红外光谱中的葡萄糖分子特异性的影响。首先测量了葡萄糖水溶液的近红外透射光谱,以葡萄糖浓度为外扰,计算二维相关同步谱及异步谱,通过对比分析确定了葡萄糖在合频和倍频区域的特征吸收峰位置。当葡萄糖水溶液中加入少量白蛋白后,其二维相关谱中葡萄糖的一级倍频吸收峰(1 590nm)和二级倍频吸收峰(1 195nm)出现了不同源的现象,表明白蛋白可能破坏了葡萄糖的特异性;进一步开展了人体口服葡萄糖耐量实验,测量了手掌部位的漫反射光谱,其二维相关谱中葡萄糖的同源性吸收也遭到破坏。另外,由于葡萄糖浓度变化引起的光谱变异信息较小,很容易被系统漂移掩盖,一般需要进行背景修正。在葡萄糖水溶液的透射实验和人体漫反射实验中,分别引入纯水样本和5%标准漫反射板作为参考,以时间为扰动的二维相关光谱分析的结果表明,经过参考样本的背景校正后,系统漂移在二维同步切线谱中引起的谱峰偏移变小,葡萄糖的特征吸收被增强。因此,在近红外光谱无创血糖检测中,应尽量避开与葡萄糖特征吸收不同源的波段,并有必要采取一定的参考测量方法以提高检测的特异性。
[Abstract]:The effects of the main components in the blood and the systematic drift during the measurement on the molecular specificity of glucose in the near infrared spectroscopy were investigated by two-dimensional correlation spectroscopy. The near infrared transmission spectra of glucose aqueous solution were measured at first. Using glucose concentration as an external disturbance, the two dimensional correlation synchronous spectra and asynchronous spectra were calculated, and the characteristic absorption peaks of glucose in the frequency-doubling and frequency-doubling regions were determined by comparative analysis. When a small amount of albumin was added to glucose aqueous solution, the first-order frequency doubling absorption peak (1 590nm) and the second order frequency doubling absorption peak (1 195nm) of glucose in the two-dimensional correlation spectrum showed different homology. It was suggested that albumin might destroy the specificity of glucose. The oral glucose tolerance test was carried out and the diffuse reflectance spectra of the palm were measured. The homologous absorption of glucose in the two-dimensional correlation spectrum was also destroyed. In addition, the spectral variation information caused by glucose concentration change is small, which is easy to be masked by system drift, so background correction is usually needed. In the transmission experiment of glucose aqueous solution and the human diffuse reflection experiment, the pure water sample and the 5% standard diffuse reflectance plate were introduced as reference respectively. After background correction of the reference sample, the peak offset caused by the system drift in the 2-D synchronous tangent spectrum is reduced, and the characteristic absorption of glucose is enhanced. Therefore, in NIR non-invasive blood glucose detection, we should avoid the band which is not homologous to the characteristic absorption of glucose, and it is necessary to adopt certain reference measurement methods to improve the specificity of the detection.
【作者单位】：天津大学精密测试技术及仪器国家重点实验室;天津大学精密仪器与光电子工程学院;
【基金】：国家自然科学基金项目(60938002) 国家(863)计划项目(2012AA022602)资助
【分类号】：R587.1;O657.3

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