辣椒素对1型糖尿病大鼠糖代谢影响作用的研究

发布时间：2018-11-18 09:03

【摘要】：目的探讨辣椒素对1型糖尿病大鼠糖代谢的影响及作用机制。方法 (1)普通大鼠设为低、中、高剂量组和空白组(n=8),剂量组分别灌胃3、6、9 mg/(kg·bw)剂量的辣椒素28 d后,检测其餐后血糖,肠道淀粉酶和葡萄糖苷酶活性;(2)建立链脲佐菌素诱导糖尿病大鼠模型,将其分为低、中、高剂量组,空白组和模型组(n=8),剂量组分别灌胃3、6、9 mg/(kg·bw)剂量辣椒素28 d后,依次检测其餐后血糖,消化酶的活性,葡萄糖耐量,血清胰岛素,肝糖元和糖化血清蛋白等与糖代谢相关指标。结果中高剂量辣椒素可显著抑制正常大鼠肠道淀粉酶和葡萄糖苷酶的活性,降低负荷(葡萄糖、蔗糖和淀粉)的餐后血糖含量,以中剂量最佳。且高剂量辣椒素可显著降低糖尿病大鼠糖化血清蛋白。结论辣椒素的降血糖机制可能通过两种途径实现:(1)通过辣椒素对肠道相关酶活性的抑制以及降低小肠对碳水化合物吸收能力,实现对肠内葡萄糖吸收的干扰;(2)刺激胰岛素的分泌和肝糖原合成,抑制糖异生,从而达到降低血糖的目的。
[Abstract]:Objective to investigate the effect and mechanism of capsaicin on glucose metabolism in type 1 diabetic rats. Methods (1) normal rats were divided into three groups: low, middle, high dose group and blank group (n = 8). After 28 days of oral administration of capsaicin, the blood glucose, intestinal amylase and glucosidase activity were measured. (2) streptozotocin induced diabetic rats were divided into low, middle and high dose groups, blank group and model group (NN8). Blood glucose, digestive enzyme activity, glucose tolerance, serum insulin, liver glycogen and glycosylated serum protein were measured in turn. Results High-dose capsaicin could significantly inhibit the activities of amylase and glucosidase in intestinal tract of normal rats and decrease the content of postprandial blood glucose (glucose, sucrose and starch) of normal rats. The middle dose of capsaicin was the best. And high dose capsaicin can significantly reduce the glycosylated serum protein in diabetic rats. Conclusion the hypoglycemic mechanism of capsaicin may be achieved in two ways: (1) by inhibiting the activity of intestinal enzymes and decreasing the absorption of carbohydrates in the small intestine, the mechanism of capsaicin may interfere with the absorption of glucose in the intestine; (2) stimulating insulin secretion and hepatic glycogen synthesis, inhibiting glucose allogenesis, thus reducing blood glucose.
【作者单位】：西南大学食品科学学院;
【基金】：国家自然科学基金项目(No.31471581)
【分类号】：R587.1

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