滑膜抗原特异性调节性T细胞的诱导扩增

发布时间：2018-11-22 14:03

【摘要】：目的诱导扩增BALB/C小鼠滑膜抗原特异性调节性T细胞(antigen specific regulatory T cells,sTregs)的产生,为探索滑膜抗原sTregs在类风湿性关节炎(rheumatoid arthritis,RA)的治疗作用提供实验基础。方法体外诱导培养小鼠骨髓源性树突状细胞(DCs)并负载Ⅱ型胶原蛋白肽,在白细胞介素-2(IL-2)的协同作用下,与小鼠脾CD4~+CD25~+自然发生的调节性T细胞(nTregs)混合培养,以刺激产生滑膜抗原sTregs,并用混合淋巴细胞抑制实验评估扩增的sTregs抑制CD4~+CD25~-T细胞增殖的功能是否具有抗原特异性。结果经DCs刺激1周后,小鼠CD4~+CD25~+Tregs扩增了6倍,并且增殖的sTregs在混合淋巴细胞抑制试验中较多克隆Tregs(Poly-Tregs)具有较强的抑制作用(P0.01),显示抗原特异性抑制的特点。结论以自身DCs负载滑膜源性抗原(Ⅱ型胶原蛋白肽)可以从小鼠脾CD4~+CD25~+nTregs中诱导扩增滑膜抗原sTregs。
[Abstract]:Objective to induce the production of synovial antigen-specific regulatory T cell (antigen specific regulatory T cells,sTregs (antigen specific regulatory T cells,sTregs) in BALB/C mice and to provide an experimental basis for exploring the therapeutic effect of synovial antigen sTregs on rheumatoid arthritis (rheumatoid arthritis,RA). Methods Murine bone marrow-derived dendritic cells (DCs) were induced and loaded with type 鈪，

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