脂肪干细胞经两种方法移植后在多囊卵巢综合征大鼠体内分布的比较

发布时间：2018-11-23 17:17

【摘要】：目的:比较观察脂肪来源间充质干细胞(ADSCs)分别经静脉和卵巢原位移植后在多囊卵巢综合征(PCOS)大鼠卵巢的归巢及其它器官的分布情况.方法:用携带绿色荧光蛋白(GFP)基因的慢病毒载体感染ADSCs,获得表达GFP的ADSCs.利用皮下注射脱氢表雄酮的方法制备PCOS大鼠模型,随机分为PCOS组(A组,n=8)、尾静脉注射组(B组,n=8)、卵巢原位注射组(C组,n=8),另选同批次正常大鼠8只设为空白对照组(D组,n=8).B、C组分别通过尾静脉和卵巢原位注射移植标记GFP的ADSCs,移植1周和1月取各组大鼠卵巢、子宫、肝、肾及肺,观察卵巢外观,各组织行冰冻切片在荧光显微镜下观察荧光分布情况.结果:慢病毒转染ADSCs48 h后,可见明显GFP表达,随着时间的推移,GFP的表达逐渐增多.B组卵巢表面未见囊肿,ADSCs散在分布于卵巢间质,子宫、肾、肝也可见ADSCs分布,但以卵巢最为多见,肺部未见明显荧光.C组卵巢注射部位可见囊性肿物,ADSCs局限分布于卵巢间质,除卵巢及部分子宫可见荧光外,其它组织未见明显荧光.两移植组移植1月及1周卵巢均可见绿色荧光标记的ADSCs.结论:移植的ADSCs可在PCOS大鼠体内存活并向卵巢归巢.通过尾静脉移植的ADSCs具有广泛分布的特点,而卵巢原位注射主要局限在卵巢局部.
[Abstract]:Aim: to compare the homing and other organ distribution of adipose derived mesenchymal stem cells (ADSCs) in the ovary of (PCOS) rats with polycystic ovary syndrome (PCOS) after intravenous and ovarian orthotopic transplantation. Methods: the ADSCs. expressing GFP was obtained by infecting ADSCs, with lentivirus vector carrying green fluorescent protein (GFP) gene. PCOS rat model was established by subcutaneous injection of dehydroepiandrosterone. The rats were randomly divided into PCOS group (group A, n = 8), caudal vein injection group (group B, n = 8) and ovarian in situ injection group (group C, n = 8). In addition, 8 normal rats of the same batch were divided into control group (group D, n = 8). The ovary, uterus, liver, kidney and lung of rats in group B were transplanted with ADSCs, labeled with GFP in caudal vein and ovary for 1 week and 1 month, respectively. The appearance of ovary was observed and the distribution of fluorescence was observed under fluorescence microscope. Results: after lentivirus was transfected into ADSCs48, the expression of GFP was obvious, and the expression of GFP increased gradually with the passage of time. In group B, no cyst was found on the surface of ovary, and ADSCs was scattered in the interstitial ovary, uterus, kidney and liver. In group C, cystic masses were found in the position of ovarian injection, and ADSCs was localized in the stroma of ovary, but no fluorescence was found in other tissues except the ovary and some uterus. Green fluorescent labeled ADSCs. was found in the ovary of both transplantation groups at 1 month and 1 week after transplantation. Conclusion: transplanted ADSCs can survive in PCOS rats and homing to ovary. ADSCs transplanted through caudal vein is widely distributed, while in situ ovarian injection is mainly localized in ovary.
【作者单位】：暨南大学附属第一医院生殖医学科;暨南大学附属第一医院健康管理中心;
【基金】：广东省科技计划项目(2014A020212223) 中央高校基本科研业务费专项资金(21615469)
【分类号】：R711.75

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