白藜芦醇在复杂性区域疼痛综合征Ⅰ型大鼠模型中的作用及机制

发布时间：2018-11-25 14:49

【摘要】：目的:探讨白藜芦醇对复杂性区域疼痛综合征Ι型(CRPS1)大鼠模型的疗效及作用机制。方法:成年雄性SD大鼠50只分为正常对照组(A组)、假手术组(B组)、模型组1(C组)、模型组2(D组),模型组3(E组)。A、B、C 3组予5%DMSO溶液,D组予MIF抑制剂ISO-1 1 mg/(kg·d),E组予白藜芦醇20 mg/(kg·d);连续腹腔注射14 d。第0、7、14天测疼痛行为,血MIF、TNF-α浓度;第14天测坐骨神经中MIF、ERK1/2和p-ERK1/2蛋白的表达。结果:第7及14天,D、E组疼痛阈值较前明显改善,血清MIF、TNF-α水平下降,坐骨神经MIF及p-ERK1/2蛋白表达明显下调,且显著低于C组(P0.05),第7天血清MIF、TNF-α已降至正常,第14天大鼠疼痛阈值也降至正常,与A组比较无明显差异(P0.05)。结论:白藜芦醇可显著改善CRPS1大鼠模型的疼痛行为,下调MIF和ERK1/2磷酸化水平的表达,通过抑制MIF/ERK信号通路干预CRPS1大鼠的疼痛行为。
[Abstract]:Aim: to investigate the effect and mechanism of resveratrol on complex regional pain syndrome type I (CRPS1) rat model. Methods: fifty adult male SD rats were divided into normal control group (group A), sham operation group (group B), model group 1 (group C), model group 2 (group D), model group 3 (group E). Group D was treated with MIF inhibitor ISO-1 1 mg/ (kg d), E group with resveratrol 20 mg/ (kg d); Continuous intraperitoneal injection for 14 days. Pain behavior and serum MIF,TNF- 伪 were measured on day 14, and the expression of MIF,ERK1/2 and p-ERK1/2 protein in sciatic nerve on day 14. Results: on the 7th and 14th days, the pain threshold was improved, the serum MIF,TNF- 伪 level was decreased, and the expression of MIF and p-ERK1/2 protein in sciatic nerve was significantly decreased, which was significantly lower than that in C group (P0.05), and on the 7th day, serum MIF, was significantly decreased. TNF- 伪 was reduced to normal, and the pain threshold was reduced to normal on the 14th day. There was no significant difference between group A and group A (P0.05). Conclusion: resveratrol can significantly improve the pain behavior of CRPS1 rats, down-regulate the expression of MIF and ERK1/2 phosphorylation, and interfere with the pain behavior of CRPS1 rats by inhibiting MIF/ERK signaling pathway.
【作者单位】：南昌大学第一附属医院风湿免疫科;
【基金】：江西省科技计划项目(编号:20132BBG70057) 江西省教育厅科学技术研究项目(编号:GJJ14012)
【分类号】：R-332;R593.2

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