马尾藻岩藻聚糖硫酸酯对高脂血症小鼠脂质代谢相关酶的影响

发布时间：2018-12-11 09:12

【摘要】：研究不同分子质量马尾藻岩藻聚糖硫酸酯对高脂血症小鼠体内脂质代谢相关酶和胆固醇合成关键酶的影响。方法:建立高脂血症小白鼠模型,将MMWF、LMWF通过体内实验灌胃小白鼠,测定血清卵磷脂胆固醇酰基转移酶(LCAT)、肝脂酶(HL)、脂蛋白脂酶(LPL)以及肝脏LCAT、LPL活性,研究其降血脂效果。结果:4种岩藻聚糖硫酸酯能显著提高高脂血症小鼠LCAT、HL、LPL活力,降低HMG-COA还原酶活力。与模型组相比,LMWF1和LMWF2各高剂量组小鼠血清LCAT活性分别提高了17.65%,27.21%,10.97%,10.51%;肝脏LCAT活性分别提高了39.73%,43.44%,27.72%和32.22%;血清HL活性分别提高了19.70%,25.76%,8.08%和19.19%;对肝脏HL活力影响不显著;血清LPL活性分别提高了47.16%,51.14%,39.77%和44.89%;肝脏LPL活性分别提高了40.22%,50%,31.52%,36.96%;血清HMG-Co A还原酶活性分别降低了36.70%,38.39%,31.20%和33.59%;肝脏HMG-Co A还原酶分别降低了18.63%,22.07%,14.54%和17.01%。结论:4种马尾藻岩藻聚糖硫酸酯通过促进脂蛋白代谢关键酶LCAT、HL和LPL活性的升高,加快TC和LDL分解代谢;或通过抑制HMG-Co A还原酶,使机体内的TC合成受阻。
[Abstract]:The effects of different molecular weight sargassum fucosan-sulfate on lipid metabolism related enzymes and cholesterol synthesis key enzymes in hyperlipidemic mice were studied. Methods: the rat model of hyperlipidemia was established. MMWF,LMWF was perfused to mice in vivo. Serum lecithin cholesterol acyltransferase (LCAT), liver lipase (HL),) lipoprotein lipase (LPL) and hepatic LCAT,LPL activity were measured. To study the effect of hypolipidemia. Results: four kinds of fucoidan sulfate could significantly increase LCAT,HL,LPL activity and decrease HMG-COA reductase activity in hyperlipidemic mice. Compared with the model group, the serum LCAT activity of LMWF1 and LMWF2 groups increased by 17.65 ~ 27.21% and 10.97% and 10.51%, respectively, and the liver LCAT activity increased by 39.73% and 32.22%, respectively. The activity of serum HL increased by 19.70% and 19.19%, and the activity of serum LPL increased by 39.77% and 44.89%, respectively, and the activity of serum LPL increased by 39.77% and 44.89%, respectively, and the activity of serum LPL was increased by 47.16%, 39.77% and 44.89%, respectively. The activity of LPL in liver was increased by 40.22 and 31.52and the activity of serum HMG-Co A reductase was decreased by 36.70% and 33.59%, respectively. HMG-Co A reductase in liver decreased 18.63% and 17.01%, respectively. Conclusion: four kinds of sargassum sulfate can accelerate the catabolism of TC and LDL by increasing the activity of LCAT,HL and LPL in lipoprotein metabolism, or inhibit the synthesis of TC by inhibiting HMG-Co A reductase.
【作者单位】：广东省水产品加工与安全重点实验室广东普通高等学校水产品深加工重点实验室广东海洋大学食品科技学院;广东海洋大学水产学院;
【基金】：广东省自然科学基金项目(2015A030313618) 湛江市科技计划项目(2014A03017)
【分类号】：R589.2

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