儿茶素和左甲状腺素钠对甲状腺功能减退大鼠抗氧化应激能力改善的影响

发布时间：2018-12-23 12:08

【摘要】：目的观察左甲状腺素钠、儿茶素以及两药联合治疗对甲状腺功能减退模型大鼠血清8-羟基脱氧鸟苷(8-OHd G)、对氧磷酶-1含量的影响以及海马组织凋亡蛋白caspase-3含量的影响。方法选取50只成年SD雄性大鼠,随机选取10只作为对照组,剩余40只用丙基硫氧密啶(PTU)诱导甲状腺功能减退大鼠模型,分为PTU组、儿茶素组、左甲状腺素钠组(L-T4组)、左甲状腺素钠+儿茶素组(联合用药组)。采用酶联免疫吸附试验测定各组大鼠血清8-OHd G、对氧磷酶-1水平。采用Western blot测定各组大鼠海马组织凋亡蛋白caspase-3的含量。结果 1与对照组比较,PTU组大鼠血清8-OHd G含量和海马组织凋亡蛋白caspase-3含量显著升高,差异均有统计学意义(P0.05);与PTU组比较,L-T4组、联合用药组大鼠血清8-OHd G含量和海马组织凋亡蛋白caspase-3含量显著降低,差异均有统计学意义(P0.05)。2与对照组比较,PTU组大鼠血清对氧磷酶-1含量显著降低,差异有统计学意义(P0.05);与PTU组比较,L-T4组、联合用药组大鼠血清对氧磷酶-1含量显著升高,差异有统计学意义(P0.05)。结论甲状腺功能减退可导致抗氧化应激能力下降以及细胞凋亡的增加,左甲状腺素钠、儿茶素药物治疗可改善氧化应激、减少细胞凋亡,两药联合使用有协同作用。
[Abstract]:Objective to observe the effects of levothyroxine sodium, catechin and two drugs on serum 8-hydroxydeoxyguanosine (8-OHd G),) in rats with hypothyroidism. The effect on the content of phosphatase-1 and the content of apoptotic protein caspase-3 in hippocampal tissue. Methods Fifty adult male SD rats were randomly selected as control group. The remaining 40 rats were induced by propyl thiomitridine (PTU). The rats were divided into PTU group and catechin group. Levothyroxine sodium group (L-T4 group), levothyroxine sodium catechin group (combined medication group). The serum levels of 8-OHd G and p-oxonase-1 were measured by enzyme-linked immunosorbent assay (Elisa). The content of apoptotic protein caspase-3 in hippocampus of rats in each group was determined by Western blot. Results 1 compared with the control group, the content of serum 8-OHd G and the content of apoptotic protein caspase-3 in hippocampus in PTU group were significantly higher than those in control group (P0.05). Compared with PTU group, serum 8-OHd G content and hippocampal apoptotic protein caspase-3 content in L-T4 group and combined treatment group were significantly lower than those in control group (P0.05). The level of serum paraoxonase-1 in PTU group was significantly lower than that in control group (P0.05). Compared with PTU group, the level of serum paraoxonase-1 in L-T4 group and combined treatment group was significantly higher than that in L-T4 group (P0.05). Conclusion hypothyroidism can decrease the ability of anti-oxidative stress and increase the apoptosis of cells. The treatment of levothyroxine sodium and catechin can improve the oxidative stress and decrease the apoptosis of cells. The combination of the two drugs has synergistic effect.
【作者单位】：安徽医科大学第二附属医院内分泌科;
【基金】：国家中医药管理局重点学科-中医内分泌学科开放基金项目(编号:2011NFMXK009)
【分类号】：R581.2

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