LC Orexin-A对肥胖抵抗大鼠自发活动的影响及机制研究

发布时间：2018-12-30 15:55

【摘要】：目的:探讨蓝斑核(LC)orexin-A对肥胖抵抗(OR)大鼠自发活动的影响及机制并进一步探究LC的orexin调控是否与肥胖抵抗相关。方法:雄性SD大鼠和选择性培育的OR大鼠,一侧LC置管,缓慢均匀注射0.5μL orexin-A或人工合成脑脊液,采用大鼠自发活动检测装置SPA红外线活动传感箱,测定大鼠自发活动量(SPA),间接测热法测定其能量消耗,定量磁共振身体组成分析器检测大鼠脂肪重和肌肉重。大鼠肥胖程度用体脂百分比表示。结果:与SD大鼠相比,OR大鼠的总体重、脂体重和去脂体重均显著降低(P0.05)。OR大鼠肥胖程度明显小于SD大鼠(P0.05)。OR大鼠在3月龄时主要表现为水平活动,6月龄时垂直活动增加,提示OR大鼠随年龄增长活动量显著增多(P0.05)。与SD大鼠相比,OR大鼠SPA水平显著增高(P0.05),非静息能量消耗显著增多(P0.05),但日间能量消耗无明显差异(P0.05)。提示,OR大鼠夜间活动增多可导致能量消耗增加,体重下降。LC注射orexin-A能明显增加OR大鼠的SPA(P0.05),并呈现剂量依赖性,但对大鼠的摄食量无明显影响(P0.05)。该结果提示,LC的orexin-A系统在OR大鼠肥胖抵抗中起着重要作用,此作用可能是通过其对SPA的调控实现的。结论:蓝斑核orexin-A对肥胖大鼠自发活动有重要影响,该作用与肥胖抵抗相关。
[Abstract]:Aim: to investigate the effect and mechanism of locus coeruleus (LC) orexin-A on spontaneous activity in obese resistant (OR) rats and to explore whether the orexin regulation of LC is related to obesity resistance. Methods: male SD rats and selectively cultured OR rats were injected with 0.5 渭 L orexin-A or synthetic cerebrospinal fluid (CSF) slowly and evenly. SPA infrared moving-sensing box was used to detect the spontaneous activity of rats. The energy consumption was measured by (SPA), indirect calorimetry and the fat weight and muscle weight were measured by quantitative magnetic resonance body composition analyzer. The degree of obesity in rats was expressed as a percentage of body fat. Results: compared with SD rats, the total weight, fat weight and degreasing weight of OR rats were significantly decreased (P0.05). OR rats were significantly less obese than SD rats (P0.05). OR rats at 3 months of age) showed horizontal activity. The vertical activity increased at 6 months, suggesting that the activity of OR rats increased significantly with age (P0.05). Compared with SD rats, SPA level of OR rats was significantly higher (P0.05), non-resting energy expenditure significantly increased (P0.05), but there was no significant difference between day energy expenditure (P0.05). The results suggested that increased night-time activity in OR rats resulted in increased energy consumption and weight loss. LC injection of orexin-A could significantly increase SPA of OR rats (P0.05) in a dose-dependent manner, but had no significant effect on the feeding intake of OR rats (P0.05). These results suggest that the orexin-A system of LC plays an important role in obesity resistance in OR rats, and this effect may be achieved through its regulation of SPA. Conclusion: orexin-A plays an important role in the spontaneous activity of obese rats, which is related to obesity resistance.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R589.2

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