狭鳕鱼皮胶原多肽对去势大鼠骨微结构影响的研究
发布时间：2019-01-02 20:21
【摘要】：目的通过观察狭鳕鱼皮胶原多肽对去势大鼠骨质疏松模型骨微结构的影响,探讨其防治骨质疏松的可行性。方法成年Wistar雌性大鼠60只,体质量(250±10)g,随机分为5组(n=12),分别为正常对照组(A组)、骨质疏松模型组(B组)、骨质疏松模型+狭鳕鱼皮胶原多肽预防组(C组)、骨质疏松模型+狭鳕鱼皮胶原多肽低剂量治疗组(D组)、骨质疏松模型+狭鳕鱼皮胶原多肽高剂量治疗组(E组),每组12只。B、C、D、E组采用摘除双侧卵巢法制备大鼠骨质疏松模型。C组从术前4周开始、D、E组从术后6周开始,每天按照1.0、0.5、1.0 g/kg行狭鳕鱼皮胶原多肽灌胃,连续6周;A、B组于术后同时间点给予等体积生理盐水灌胃。末次给药24 h后,A、B组大鼠摄股骨X线片并测定灰度值;然后颈椎脱臼法处死各组大鼠,取左侧胫骨近端骨组织行HE染色,观察骨组织病理学改变,测量骨小梁数量(trabecular number,TN)、平均骨小梁厚度(mean trabecular plate thickness,MTPT)、平均骨小梁间距(mean trabecular plate spacing,MTPS)、骨小梁体积百分比(trabecular bone volume,TBV)、平均骨皮质厚度(mean bone cortical thickness,MBCT);免疫组织化学染色观测降钙素受体(caltitonin receptor,CTR)及IL-1表达水平。结果 B组大鼠股骨灰度值显著低于A组(t=45.130,P=0.000),表明去势大鼠骨质疏松模型制备成功。组织学观察示,A、C、E组TN、MTPS、TBV、MBCT与B组比较,差异有统计学意义(P0.05);C组各骨组织形态计量学参数与D、E组比较,差异均有统计学意义(P0.05);D组TN、MTPS、TBV、MBCT与A组比较差异有统计学意义(P0.05);E组仅MTPS与A组比较差异有统计学意义(P0.05);E组MTPS、TBV、MBCT与D组比较,差异有统计学意义(P0.05)。免疫组织化学染色观察示,A、C、D、E组CTR、IL-1表达水平较B组降低,C、E组低于D组,差异有统计学意义(P0.05);其余组间比较差异均无统计学意义(P0.05)。结论狭鳕鱼皮胶原多肽能够改善骨质疏松大鼠的骨微结构,其机制可能与抑制骨组织中CTR、IL-1表达有关,但尚未发现其对骨质疏松症有预防作用。
[Abstract]:Objective to observe the effect of collagen peptide from cod skin on bone microstructure of ovariectomized rats and to explore the feasibility of prevention and treatment of osteoporosis. Methods Sixty adult Wistar female rats, weighing (250 卤10) g, were randomly divided into 5 groups (n = 12): normal control group (group A) and osteoporosis model group (group B). Osteoporosis model cod skin collagen peptide prevention group (C group), osteoporosis model cod skin collagen peptide low dose treatment group (D group), osteoporosis model narrow cod skin collagen peptide high dose treatment group (E group), osteoporosis model cod skin collagen peptide prevention group (group C), osteoporosis model cod skin collagen peptide low dose treatment group (group D), Osteoporosis model was established in each group with 12 rats. The osteoporosis model was established in group C from 4 weeks before operation and from 6 weeks after operation in group D. Collagen polypeptide from cod skin was perfused daily for 6 weeks according to 1.0 g/kg. Group B was given the same volume of normal saline at the same time after operation. Twenty-four hours after the last administration, the rats in group A and B took X-ray films of femur and measured the gray value. Then the rats were killed by cervical dislocated method. The left proximal tibia bone tissue was taken for HE staining. The histopathological changes of bone were observed. The number of trabecular bone (trabecular number,TN) and the mean trabecular thickness (mean trabecular plate thickness,MTPT) were measured. Mean trabecular spacing (mean trabecular plate spacing,MTPS), trabecular volume percentage (trabecular bone volume,TBV), and mean cortical thickness (mean bone cortical thickness,MBCT); The expression of calcitonin receptor (caltitonin receptor,CTR) and IL-1 were detected by immunohistochemical staining. Results the gray value of femur in group B was significantly lower than that in group A (t = 45.130), which indicated that the model of osteoporosis in ovariectomized rats was successfully prepared. Histological observation showed that there was significant difference in TN,MTPS,TBV,MBCT between group A and group B (P0.05). The bone histomorphometry parameters in); C group were significantly different from those in group E (P0.05). The difference of TN,MTPS,TBV,MBCT between group D and group A was statistically significant (P0.05). Only MTPS in group); E was significantly different from group A (P0.05). MTPS,TBV,MBCT in group E was significantly different from that in group D (P0.05). Immunohistochemical staining showed that the expression of CTR,IL-1 in group A was lower than that in group B, and that in group C was lower than that in group D (P0.05). There was no significant difference between the other groups (P0.05). Conclusion the collagen peptide from cod skin can improve the bone microstructure of osteoporosis rats, and its mechanism may be related to the inhibition of CTR,IL-1 expression in bone tissue, but it has not been found to have a preventive effect on osteoporosis.
【作者单位】： 青岛大学医学部中西医结合中心;青岛大学医学部解剖学教研室;青岛大学医学部临床医学院;青岛市海慈医疗集团关节外科;青岛大学药学院;
【分类号】：R580
，
本文编号：2398939
[Abstract]:Objective to observe the effect of collagen peptide from cod skin on bone microstructure of ovariectomized rats and to explore the feasibility of prevention and treatment of osteoporosis. Methods Sixty adult Wistar female rats, weighing (250 卤10) g, were randomly divided into 5 groups (n = 12): normal control group (group A) and osteoporosis model group (group B). Osteoporosis model cod skin collagen peptide prevention group (C group), osteoporosis model cod skin collagen peptide low dose treatment group (D group), osteoporosis model narrow cod skin collagen peptide high dose treatment group (E group), osteoporosis model cod skin collagen peptide prevention group (group C), osteoporosis model cod skin collagen peptide low dose treatment group (group D), Osteoporosis model was established in each group with 12 rats. The osteoporosis model was established in group C from 4 weeks before operation and from 6 weeks after operation in group D. Collagen polypeptide from cod skin was perfused daily for 6 weeks according to 1.0 g/kg. Group B was given the same volume of normal saline at the same time after operation. Twenty-four hours after the last administration, the rats in group A and B took X-ray films of femur and measured the gray value. Then the rats were killed by cervical dislocated method. The left proximal tibia bone tissue was taken for HE staining. The histopathological changes of bone were observed. The number of trabecular bone (trabecular number,TN) and the mean trabecular thickness (mean trabecular plate thickness,MTPT) were measured. Mean trabecular spacing (mean trabecular plate spacing,MTPS), trabecular volume percentage (trabecular bone volume,TBV), and mean cortical thickness (mean bone cortical thickness,MBCT); The expression of calcitonin receptor (caltitonin receptor,CTR) and IL-1 were detected by immunohistochemical staining. Results the gray value of femur in group B was significantly lower than that in group A (t = 45.130), which indicated that the model of osteoporosis in ovariectomized rats was successfully prepared. Histological observation showed that there was significant difference in TN,MTPS,TBV,MBCT between group A and group B (P0.05). The bone histomorphometry parameters in); C group were significantly different from those in group E (P0.05). The difference of TN,MTPS,TBV,MBCT between group D and group A was statistically significant (P0.05). Only MTPS in group); E was significantly different from group A (P0.05). MTPS,TBV,MBCT in group E was significantly different from that in group D (P0.05). Immunohistochemical staining showed that the expression of CTR,IL-1 in group A was lower than that in group B, and that in group C was lower than that in group D (P0.05). There was no significant difference between the other groups (P0.05). Conclusion the collagen peptide from cod skin can improve the bone microstructure of osteoporosis rats, and its mechanism may be related to the inhibition of CTR,IL-1 expression in bone tissue, but it has not been found to have a preventive effect on osteoporosis.
【作者单位】： 青岛大学医学部中西医结合中心;青岛大学医学部解剖学教研室;青岛大学医学部临床医学院;青岛市海慈医疗集团关节外科;青岛大学药学院;
【分类号】：R580
，
本文编号：2398939
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