氯化汞对大鼠血管内皮损伤的毒性研究
发布时间：2019-01-04 12:54
【摘要】：目的研究不同剂量氯化汞对SD大鼠的血管内皮损伤情况。方法将54只雄性SD大鼠简单随机分为四组,高、中、低氯化汞中毒组,每组15只,对照组9只;采用高(17 mg·kg~(-1)·d~(-1))、中(8.5 mg·kg~(-1)·d~(-1))、低剂量(4.25 mg·kg~(-1)·d~(-1))浓度氯化汞盐水溶液灌胃建立SD大鼠亚急性汞中毒动物模型。中毒后7 d、14 d、21 d分别测定大鼠血细胞汞值、血清一氧化氮(NO)和内皮素-1(ET-1)值,同时行循环内皮细胞(CEC)计数。结果对照组血细胞汞值接近于零,中毒组与对照组差异均有统计学意义(P0.05)。血清ET-1组间比较,高剂量组与对照组差异有统计学意义(P0.05);组内比较,不同时期ET-1值差异有统计学意义(P0.05)。血清NO组间比较,各剂量组与对照组差异均有统计学意义(P0.05);同期比较,不同中毒剂量组之间比较差异也有统计学意义(P0.05);组内比较,不同时期之间差异均有统计学意义(P0.05)。血循环内皮细胞计数组间比较,各剂量组与对照组差异均有统计学意义(P0.05);组内比较,不同时期之间差异均有统计学意义(P0.05)。结论氯化汞可对血管内皮细胞有直接损伤作用,且损伤作用与剂量、时间存在同一变化趋势。
[Abstract]:Objective to study the vascular endothelial injury induced by different doses of mercuric chloride in SD rats. Methods 54 male SD rats were randomly divided into four groups: high, medium and low mercuric chloride poisoning group (n = 15) and control group (n = 9). In high (17 mg kg~ (-1) d ~ (-1),) (8.5 mg kg~ (-1) d ~ (-1),) Low dose (4.25 mg kg~ (-1) d ~ (-1) mercuric chloride solution was used to establish subacute mercury poisoning model of SD rats. The mercury levels in blood cells, serum nitric oxide (NO) and endothelin-1 (ET-1) levels were measured on the 7th day, 14th day and 21st day after poisoning. The circulating endothelial cells (CEC) were counted at the same time. Results the mercury level of blood cells in the control group was close to zero, and the difference between the poisoning group and the control group was statistically significant (P0.05). There were significant differences in serum ET-1 between high dose group and control group (P0.05); in intra-group comparison, there were significant differences in ET-1 values in different periods (P0.05). There were significant differences in serum NO between each dose group and the control group (P0.05), and there were significant differences between the different toxic dose groups (P0.05) in the same period. Comparison within the group, the differences between different periods were statistically significant (P0.05). Blood circulation endothelial cell count between the group, each dose group and the control group were statistically significant difference (P0.05); intra-group comparison, the differences between different periods were statistically significant (P0.05). Conclusion Mercury chloride can directly injure vascular endothelial cells, and the damage has the same trend as dose and time.
【作者单位】： 军事医学科学院附属医院神经内科;
【基金】：国家科技支撑计划课题(编号:2012BAI38B01)
【分类号】：R114
本文编号：2400320
[Abstract]:Objective to study the vascular endothelial injury induced by different doses of mercuric chloride in SD rats. Methods 54 male SD rats were randomly divided into four groups: high, medium and low mercuric chloride poisoning group (n = 15) and control group (n = 9). In high (17 mg kg~ (-1) d ~ (-1),) (8.5 mg kg~ (-1) d ~ (-1),) Low dose (4.25 mg kg~ (-1) d ~ (-1) mercuric chloride solution was used to establish subacute mercury poisoning model of SD rats. The mercury levels in blood cells, serum nitric oxide (NO) and endothelin-1 (ET-1) levels were measured on the 7th day, 14th day and 21st day after poisoning. The circulating endothelial cells (CEC) were counted at the same time. Results the mercury level of blood cells in the control group was close to zero, and the difference between the poisoning group and the control group was statistically significant (P0.05). There were significant differences in serum ET-1 between high dose group and control group (P0.05); in intra-group comparison, there were significant differences in ET-1 values in different periods (P0.05). There were significant differences in serum NO between each dose group and the control group (P0.05), and there were significant differences between the different toxic dose groups (P0.05) in the same period. Comparison within the group, the differences between different periods were statistically significant (P0.05). Blood circulation endothelial cell count between the group, each dose group and the control group were statistically significant difference (P0.05); intra-group comparison, the differences between different periods were statistically significant (P0.05). Conclusion Mercury chloride can directly injure vascular endothelial cells, and the damage has the same trend as dose and time.
【作者单位】： 军事医学科学院附属医院神经内科;
【基金】：国家科技支撑计划课题(编号:2012BAI38B01)
【分类号】：R114
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