富血小板血浆联合人脐带间充质干细胞移植对大鼠骨质疏松骨折愈合的影响

发布时间：2019-01-07 11:59

【摘要】：目的探讨PRP联合h UC-MSCs移植对大鼠骨质疏松性骨折愈合的影响。方法雌性SPF级SD大鼠50只随机分为5组,每组10只:假手术组(仅切除卵巢周围组织),安慰剂组(注射生理盐水),未诱导组(给予未诱导的h UC-MSCs),成骨诱导组(移植诱导后的h UC-MSCs),PRP+成骨诱导组(移植诱导后的h UC-MSCs联合PRP)。OPF造模成功后,对各组动物进行细胞移植3次,分别在OPF造模后1周、3周和7周。骨折后的第1周、3周、5周、7周、11周、13周Micro CT扫描动物模型患肢。结果 Micro CT动态观察结果显示,PRP+成骨诱导组骨痂生成量明显多于、早于其余各组,骨折11周时达到临床愈合标准,而其他组别骨痂重塑期相对滞后,比正常骨量大鼠愈合时间至少推迟了37.5%。结论 PRP联合h UC-MSCs移植可促进大鼠骨质疏松性骨折愈合,为临床治疗OPF提供数据支持。
[Abstract]:Objective to investigate the effect of PRP combined with h UC-MSCs transplantation on osteoporotic fracture healing in rats. Methods Fifty female SD rats of SPF grade were randomly divided into 5 groups: sham operation group (ovariectomy only), placebo group (saline injection) and uninduced group (uninduced h UC-MSCs). Osteoblast induction group (h UC-MSCs), PRP osteoblastic induction group after transplantation induced by h UC-MSCs combined with PRP). OPF model was successfully established, 3 times of cell transplantation, 1 week, 3 weeks and 7 weeks after OPF modeling, respectively). Micro CT was used to scan the affected limbs at week 1, week 3, week 5, week 7, week 11 and week 13 after fracture. Results the dynamic observation of Micro CT showed that the callus production in the PRP osteoblast induction group was more than that in the other groups, which was earlier than that in the other groups, and reached the clinical healing standard at 11 weeks after fracture, while the callus remodeling phase in the other groups was relatively delayed. The healing time of rats with normal bone mass was delayed by at least 37.5%. Conclusion PRP combined with h UC-MSCs transplantation can promote osteoporotic fracture healing in rats and provide data support for clinical treatment of OPF.
【作者单位】：广东医学院附属医院骨科中心;广东医学院附属医院干细胞研发与细胞治疗中心;
【基金】：广东省自然科学基金项目(S2013020012866) 广东省医学科研基金项目(B2014313) 湛江市科技计划项目(2014A06005) 广东医学院科研基金项目(Z2013005)
【分类号】：R580;R683

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