组织型转谷氨酰胺酶抗体在1型糖尿病患者中表达的意义
发布时间：2019-01-17 18:47
【摘要】：目的:检测TIDM患者血清中t TGA、GADA、Zn T8A及IA-2A的水平,探讨其临床意义。方法:选取自2013年3月至2014年10月在青海大学附属医院、青海省人民医院、青海省红十字医院内分泌科住院的T1DM患者共计42例,男性19例,女性23例,新诊断的T2DM患者38例,男性20例,女性18例,此外,选择41例健康成年人作为对照组,T1DM、T2DM和对照组均在清晨空腹抽静脉血,静脉血经离心后收集,应用放射配体法检测其t TGA、GADA、Zn T8A及IA-2A水平,计算其抗体指数,进行统计学分析。结果:T1DM患者血清的t TGA、GADA、Zn T8A及IA-2A抗体指数和阳性率均高于T2DM组和对照组,差异具有统计学意义(P0.05);在T1DM中,t TGA抗体阳性率高于GADA、IA-2A、Zn T8A,差异具有统计学意义(P0.05),在T1DM中,t TGA的灵敏度优于GADA、IA-2A、Zn T8A,差异具有统计学意义(P0.01)。结论:T1DM中,t TGA、GADA、IA-2A、Zn T8A抗体指数及阳性率显著高于T2DM和对照组,这些抗体阳性的人群应定期监测血糖,以便早发现早治疗。在T1DM中,t TGA与GADA、IA-2A、Zn T8A的特异度相似,但灵敏度优于GADA、IA-2A及Zn T8A,在临床应用中可能更有价值。
[Abstract]:Objective: to investigate the clinical significance of t TGA,GADA,Zn T 8 A and IA-2A in serum of TIDM patients. Methods: from March 2013 to October 2014, 42 T1DM patients, including 19 males, 23 females, 38 newly diagnosed T2DM patients, were selected from Qinghai Provincial people's Hospital, Qinghai Provincial people's Hospital and Qinghai Red Cross Hospital. There were 20 males and 18 females. In addition, 41 healthy adults were selected as control group. Both T1DMN T2DM and control group received venous blood on an empty stomach in the morning. The venous blood was collected after centrifugation, and its t TGA,GADA, was detected by radioligand assay. The levels of Zn T 8A and IA-2A were calculated and the antibody index was analyzed statistically. Results: the serum t TGA,GADA,Zn T8A and IA-2A antibody index and positive rate of T1DM patients were higher than those of T2DM group and control group (P0.05). The positive rate of, t TGA antibody in T1DM was higher than that in GADA,IA-2A,Zn T8A, the difference was statistically significant (P0.05), the sensitivity of, t TGA in T1DM was better than that of GADA,IA-2A,Zn T8A, and the difference was statistically significant (P0.01). Conclusion: the antibody index and positive rate of t TGA,GADA,IA-2A,Zn T8A in T1DM are significantly higher than those in T2DM and control group. The specificity of, t TGA and GADA,IA-2A,Zn T8A in T1DM is similar, but the sensitivity is superior to that of GADA,IA-2A and Zn T8A, which may be more valuable in clinical application.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.1
本文编号：2410267
[Abstract]:Objective: to investigate the clinical significance of t TGA,GADA,Zn T 8 A and IA-2A in serum of TIDM patients. Methods: from March 2013 to October 2014, 42 T1DM patients, including 19 males, 23 females, 38 newly diagnosed T2DM patients, were selected from Qinghai Provincial people's Hospital, Qinghai Provincial people's Hospital and Qinghai Red Cross Hospital. There were 20 males and 18 females. In addition, 41 healthy adults were selected as control group. Both T1DMN T2DM and control group received venous blood on an empty stomach in the morning. The venous blood was collected after centrifugation, and its t TGA,GADA, was detected by radioligand assay. The levels of Zn T 8A and IA-2A were calculated and the antibody index was analyzed statistically. Results: the serum t TGA,GADA,Zn T8A and IA-2A antibody index and positive rate of T1DM patients were higher than those of T2DM group and control group (P0.05). The positive rate of, t TGA antibody in T1DM was higher than that in GADA,IA-2A,Zn T8A, the difference was statistically significant (P0.05), the sensitivity of, t TGA in T1DM was better than that of GADA,IA-2A,Zn T8A, and the difference was statistically significant (P0.01). Conclusion: the antibody index and positive rate of t TGA,GADA,IA-2A,Zn T8A in T1DM are significantly higher than those in T2DM and control group. The specificity of, t TGA and GADA,IA-2A,Zn T8A in T1DM is similar, but the sensitivity is superior to that of GADA,IA-2A and Zn T8A, which may be more valuable in clinical application.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.1
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