饮水补充硼对雄性肥胖大鼠血清生化指标和免疫器官的影响

发布时间：2019-01-19 15:41

【摘要】：目的研究饮水补充硼对雄性肥胖大鼠血清生化指标及免疫器官组织结构的影响。方法选用40只3月龄清洁级雄性SD大鼠,随机分为5组:空白对照组、肥胖模型组和硼低、中、高剂量组,空白对照组饲喂普通饲料,其余4组饲喂高脂饲料,持续8周造模。随后在硼低、中、高剂量组大鼠饮水中添加20、40和80 mg/L硼,空白对照组和肥胖模型组饮用蒸馏水,试验期90 d。试验结束后,右心房采血测定肝功能相关生化指标,同时称重胸腺和脾脏,HE染色,显微观察并测定免疫器官组织学参数。结果与空白对照组相比,肥胖模型组大鼠Lee's指数和腹脂率、血清低密度脂蛋白胆固醇(LDL-c)含量以及胸腺重量均显著增加(P0.05),而血清总蛋白(TP)和高密度脂蛋白胆固醇(HDL-c)含量则显著降低(P0.05)。与肥胖模型组相比,低剂量硼添加组大鼠Lee's指数和腹脂率、血清载脂蛋白B(apo B)和LDL-c含量、胸腺重量及指数均显著降低(P0.05),而血清TP和HDL-c含量均显著增加(P0.05);硼中剂量组大鼠血清LDL-c含量和胸腺重量也显著降低(P0.05);但硼高剂量组大鼠上述检测指标均无显著变化。显微观测可见,硼低、中剂量组脾小结面积显著增加(P0.05),脾动脉周围淋巴鞘和边缘区增厚,脾索增粗,胸腺髓质/皮质比例显著降低(P0.05),细胞排列紧密,髓质内空泡样结构较少,硼高剂量组脾脏和胸腺组织结构无明显变化。结论饮水补充20 mg/L硼可明显降低血清apo B和LDL-c水平,增加HDL-c水平,缓解高脂饮食引发的肥胖对肝功能相关指标及免疫器官的损伤。
[Abstract]:Objective to study the effects of drinking water boron supplementation on serum biochemical indexes and immune organ structure in obese male rats. Methods Forty 3-month-old clean grade male SD rats were randomly divided into five groups: blank control group, obesity model group, low, medium, high dose group, blank control group, and the other 4 groups were fed with high fat diet for 8 weeks. Then the rats of low, medium and high doses of boron were added 2040 and 80 mg/L boron in drinking water, and the blank control group and obesity model group drank distilled water for 90 days. At the end of the experiment, the right atrium blood was collected to determine the biochemical indexes related to liver function, the thymus and spleen were weighed, HE staining was performed, and the histological parameters of immune organs were observed and measured microscopically. Results compared with the control group, the Lee's index, abdominal fat rate, serum low density lipoprotein cholesterol (LDL-c) content and thymus weight in the obese model group were significantly increased (P0.05). The contents of serum total protein (TP) and high density lipoprotein cholesterol (HDL-c) were significantly decreased (P0.05). Compared with the obese model group, the Lee's index, abdominal fat rate, serum apolipoprotein B (apo B) and LDL-c content, thymus weight and index in the low dose boron group were significantly decreased (P0.05). The levels of serum TP and HDL-c increased significantly (P0.05). The content of serum LDL-c and thymus weight in the middle dose group of boron also decreased significantly (P0.05), but there was no significant change in the above indexes in the high dose group of boron. Microscopic observation showed that the area of splenic nodules was significantly increased (P0.05), the peripheral lymphatic sheath and marginal region of splenic artery thickened, the spleen cord thickened, the medulla / cortex ratio of thymus significantly decreased (P0.05), and the cells were arranged closely in the middle dose group (P0.05). There were few vacuolar structures in medulla, but no significant changes in spleen and thymus tissues in high dose boron group. Conclusion drinking water supplementation with boron for 20 mg/L can significantly decrease serum apo B and LDL-c levels, increase HDL-c level, and alleviate liver function related indexes and immune organ damage caused by obesity induced by high fat diet.
【作者单位】：安徽科技学院动物科学学院;
【基金】：国家自然科学基金(No.31402154,31502137,31501968,31672502) 安徽省农业科技成果转化资金(No.1504032001) 安徽科技学院稳定人才基金(No.ZRC2013354)安徽科技学院兽医学重点学科项目(No.AKZDXK2015A04) 国家级大学生创新课题(No.201610879001):安徽省第七批“115”产业创新团队项目
【分类号】：R589.2

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