罗格列酮对OLETF大鼠肝脏组织病理变化及白细胞介素-17、Bcl-xl表达的影响

发布时间：2019-01-27 13:51

【摘要】：目的探讨罗格列酮(RGZ)对2型糖尿病(T2DM)OLETF大鼠肝脏组织病理变化及白细胞介素(IL)-17、Bcl-xl的影响。方法取OLETF雄性T2DM大鼠16只,随机分为T2DM组和RGZ组,正常对照组(NC)组为LETO鼠,每组8只。给药12 w后同时采集肝脏组织,进行HE、Mass、PAS染色观察组织学改变,免疫组织化学染色观察IL-17和Bcl-xl阳性细胞。结果 T2DM组大鼠肝小叶结构紊乱,可见大量胶原纤维。肝细胞脂肪空泡形成、糖原沉积减少、细胞凋亡增加,Bcl-xl蛋白表达降低、炎症因子IL-17增加。RGZ组较T2DM组肝细胞损伤明显减轻。结论 T2DM可致肝脏组织学发生损伤,而罗格列酮可减轻这种损伤的发生。
[Abstract]:Objective to investigate the effect of rosiglitazone (RGZ) on hepatic histopathological changes and interleukin-17 (IL) Bcl-xl in rats with type 2 diabetes mellitus (T2DM). Methods Sixteen OLETF male T2DM rats were randomly divided into T2DM group and RGZ group. The normal control group (NC) group was LETO rats with 8 rats in each group. After 12 weeks of administration, liver tissues were collected at the same time, HE,Mass,PAS staining was used to observe the histological changes, and IL-17 and Bcl-xl positive cells were observed by immunohistochemical staining. Results in T2DM group, the hepatic lobular structure was disordered and a large number of collagen fibers were observed. Hepatocyte fat vacuole formation, glycogen deposition decreased, apoptosis increased, Bcl-xl protein expression decreased, and inflammatory factor IL-17 increased. The damage of hepatocytes in RGZ group was significantly reduced than that in T2DM group. Conclusion T2DM can induce liver histologic injury, and rosiglitazone can reduce it.
【作者单位】：天津医科大学组织胚胎学教研室;
【分类号】：R587.1

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