高脂血症血清脂肪酸标志物的筛选研究

发布时间：2019-02-12 16:29

【摘要】：据有关数据显示,我国高血脂患者的数量非常庞大,每年国家花费在此类疾病上的医疗资源和财力数额巨大。即使如此,我国对于高脂血症到目前为止还是处于被动应付的处境,无法有效解决困扰广大人民群众的健康问题。追其原因,主要是由于缺乏有效的早期检查和判别的方法。如果能够及早发现、及早干预治疗,就可以将疾病扼杀在摇篮里。而如果一直待到生化指标检测发现异常时,那时人体已处于患病状态,只能接受药物治疗。因此我们需要找到在疾病初期就能够检查和评判的方法,而不是在疾病发生后进行药物治疗。脂质组学的出现满足了这一要求,其致力于在疾病发生初期就检测出异常代谢物,然后采用日常饮食调节来达到预防疾病目的,而不是在疾病发生后通过药物进行调控和治疗。本论文采用脂质组学的研究思路,选择GC-MS检测技术,检测高脂血症动态模型中的小鼠血清脂肪酸的动态变化情况,希望通过观测动态模型小鼠血清脂肪酸的差异,找到与高脂血症有关的一些基础信息。本论文得到了如下结论:(1)通过对不同时间点的实验小鼠血清进行生化检测以及相应时间点的实验小鼠肝脏做病理切片,可以清晰的观察到随着饲喂高脂饲料的时间拉长,实验小鼠高脂血症症状越来越明显,说明高脂血症时间动态模型建立成功。(2)对前期建立的时间动态模型中的实验小鼠血清进行GC-MS检测。此次采取的是通过饲喂高脂饲料时间不同,使各组之间高脂血症症状产生一个动态过程。分析检测结果,发现各组之间的脂肪酸含量都不相同,且都具有一定的显著性,但是只有三种脂肪酸的变化是有规律性的,和时间动态是一致的,这三种脂肪酸是:C16:1、C18:1、C22:6。(3)在实验室前期实验的基础上,对高脂组小鼠,基础组小鼠,以及灌胃过有降脂功效的功能性油脂的高脂组小鼠血清进行GC-MS检测分析。此次是通过饲喂饲料不同,使各组之间也产生动态效果,与时间动态模型形成对照。得到各组之间血清脂肪酸含量都有一定的差异,但是其中C16:1、C18:1、C22:6三种脂肪酸在这三组之间的变化差异显著,且具有一定的规律性,可能为潜在的生物标志物。
[Abstract]:According to the relevant data, the number of hyperlipidemia patients in China is very large, and the amount of medical resources and financial resources that the country spends on such diseases is huge every year. Even so, our country is still in a passive state of coping with hyperlipidemia so far, which can not effectively solve the health problems besetting the masses of the people. The main reason is the lack of effective early detection and discrimination methods. Early detection and early intervention can stifle the disease in its cradle. And if you stay until biochemical tests find abnormal, by that time the body is in a state of illness, can only be treated with drugs. So we need to find a way to check and judge at the beginning of the disease, not after the disease. The appearance of lipid genomics satisfies this requirement. It is committed to detecting abnormal metabolites in the early stages of the disease, and then using diet to prevent the disease, rather than drug control and treatment after the disease occurs. In this paper, we choose the GC-MS technique to detect the dynamic changes of serum fatty acids in the dynamic model of hyperlipidemia, and hope to observe the difference of the fatty acids in the serum of the dynamic model mice. Find some basic information about hyperlipidemia. The conclusions of this paper are as follows: (1) by biochemical detection of serum at different time points and pathological section of liver of experimental mice at corresponding time points, it can be clearly observed that the time of feeding high fat feed is lengthened. The symptom of hyperlipidemia in experimental mice was more and more obvious, which indicated that the time dynamic model of hyperlipidemia was established successfully. (2) the serum of experimental mice was detected by GC-MS. This time, the hyperlipidemia symptoms were produced a dynamic process by feeding hyperlipidemia feed for different time. The results of analysis showed that the fatty acid contents of each group were different and had some significance, but only three kinds of fatty acids were regular and consistent with the time dynamics. The three fatty acids were: C16: 1, C18: 1 and C22: 6. (3) on the basis of laboratory experiments, the serum of mice in high fat group, basic group and hyperlipidemic group were detected by GC-MS. By feeding different feed, the dynamic effect of each group was also produced, which was contrasted with the time dynamic model. The results showed that there were some differences in serum fatty acid contents among the three groups, but C16: 1C18: 1: C22: 6 had significant difference among the three groups, and had certain regularity, which might be a potential biomarker.
【学位授予单位】：武汉轻工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R589.2

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