亚硒酸钠与核黄素联合暴露对高脂饮食大鼠血脂及血清肝生化指标的影响

发布时间：2019-02-16 21:02

【摘要】：目的研究亚硒酸钠与核黄素联合暴露对高脂饮食雄性大鼠血脂及血清肝生化指标的影响。方法将60只健康SPF级雄性SD大鼠按体重随机分为空白对照组(10只)和高脂组(50只),分别给予基础饲料和高脂饲料;喂养4周后,将50只高脂组大鼠按照血脂水平和体重随机分为5组,分别为高脂对照组、1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组、18.40μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组、1.84μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组和18.40μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组,每组10只。采用灌胃方式进行染毒,同时,空白对照组和高脂对照组给予相同体积的生理盐水,染毒容量为10 ml/kg,每天1次,连续60 d。分别于第0(染毒前)、20、40、60天,测定大鼠血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)水平;并于第60天,检测大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转氨酶(AST)的水平。结果与空白对照组比较,染毒期间高脂对照组及各干预组大鼠血清TC、TG、LDL-C的水平均升高,除第60天时1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组大鼠血清TG水平外,差异有统计学意义(P0.05);而血清HDL-C水平均未见显著变化。与高脂对照组相比,第20、40天时1.84μg/kg亚硒酸钠+0.70mg/kg核黄素干预组大鼠血清TC、TG的水平及1.84μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组大鼠血清TG的水平以及第60天时1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组大鼠血清TC、TG、LDL-C的水平及18.40μg/kg亚硒酸钠+0.70mg/kg核黄素干预组大鼠血清TC的水平均下降,差异有统计学意义(P0.05);而各干预组大鼠血清HDL的水平均无明显改变。与空白对照组比较,高脂对照组、18.40μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组和1.84μg/kg亚硒酸钠+3.50mg/kg核黄素干预组大鼠血清ALT的水平以及高脂对照组、18.40μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组和18.40μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组大鼠血清AST的水平均升高,差异有统计学意义(P0.05)。与高脂对照组相比,仅1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组大鼠血清ALT的水平降低,差异有统计学意义(P0.05);1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组、18.40μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组以及1.84μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组大鼠血清AST的水平降低,差异均有统计学意义(P0.05)。结论适量亚硒酸钠与核黄素联合暴露可以拮抗高脂饮食所致雄性大鼠血脂及血清肝生化指标的升高。
[Abstract]:Objective to study the effects of sodium selenite combined with riboflavin exposure on serum lipids and liver biochemical indexes in high fat diet male rats. Methods 60 healthy SPF male SD rats were randomly divided into control group (n = 10) and hyperlipidemia group (n = 50) according to their body weight. After 4 weeks of feeding, 50 rats in hyperlipidemia group were randomly divided into 5 groups according to blood lipid level and body weight. The rats were divided into 5 groups: hyperlipidemia control group, 1.84 渭 g/kg sodium selenite 0.70 mg/kg riboflavin intervention group. 18.40 渭 g/kg sodium selenite 0.70 mg/kg riboflavin intervention group, 1.84 渭 g/kg sodium selenite 3.50 mg/kg riboflavin intervention group and 18.40 渭 g/kg sodium selenite 3.50 mg/kg riboflavin intervention group, 10 rats in each group. At the same time, the blank control group and the high-fat control group were given the same volume of normal saline once a day for 10 ml/kg, for 60 days. The serum levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) in serum of rats were measured on day 0 (before exposure) and day 40 (60 d), respectively. The level of serum alanine aminotransferase (ALT),) aspartate aminotransferase (AST) was measured on the 60th day. Results compared with the blank control group, the serum TC,TG,LDL-C level in the hyperlipidemia control group and the intervention group increased during the exposure period. There was a significant difference in serum TG level between the rats of 0.70 mg/kg riboflavin intervention group and 1.84 渭 g/kg sodium selenite intervention group on the 60th day (P0.05). There was no significant change in serum HDL-C level. Compared with the hyperlipidemia control group, the serum TC, of rats treated with sodium selenite 0.70mg/kg riboflavin at day 2040 was 1.84 渭 g/kg. TG level and serum TG level of 1.84 渭 g/kg sodium selenite 3.50 mg/kg riboflavin intervention group and 1.84 渭 g/kg sodium selenite 0.70 mg/kg riboflavin intervention group on the 60th day The level of LDL-C and the level of serum TC in the intervention group of 18.40 渭 g/kg sodium selenite 0.70mg/kg riboflavin decreased significantly (P0.05). There was no significant change in serum HDL level in all intervention groups. Compared with the blank control group, the serum ALT levels in hyperlipidemia control group, 18.40 渭 g/kg sodium selenite 0.70 mg/kg riboflavin intervention group and 1.84 渭 g/kg selenite sodium selenite riboflavin group and hyperlipidemia control group were compared. The serum AST levels of 18.40 渭 g/kg sodium selenite 0.70 mg/kg riboflavin intervention group and 18.40 渭 g/kg sodium selenite 3.50 mg/kg riboflavin group increased significantly (P0.05). Compared with the high-fat control group, only 1.84 渭 g/kg sodium selenite 0.70 mg/kg riboflavin intervention group decreased the level of serum ALT, the difference was statistically significant (P0.05). The levels of serum AST in the 渭 g/kg sodium selenite 0.70 mg/kg riboflavin intervention group, 18.40 渭 g/kg sodium selenite 0.70 mg/kg riboflavin intervention group and 1.84 渭 g/kg sodium selenite 3.50 mg/kg riboflavin intervention group were decreased. The difference was statistically significant (P0.05). Conclusion combined exposure of sodium selenite and riboflavin can antagonize the increase of serum lipids and liver biochemical indexes in male rats induced by high fat diet.
【作者单位】：天津医科大学公共卫生学院;天津市疾病预防控制中心毒理室;
【基金】：2013年天津市卫生行业重点攻关项目(13KG138)
【分类号】：R589.2

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