维生素C治疗百草枯中毒大鼠的量效关系

发布时间：2019-03-03 13:18

【摘要】：目的:探讨维生素C治疗百草枯(paraquat,PQ)中毒大鼠的疗效及量效关系。方法:将40只SpragueDawley(SD)大鼠随机分为对照组、中毒组和维生素C1组、C2组,每组10只。通过胃内一次性灌注150 mg/kg PQ,建立PQ中毒SD大鼠模型。中毒组给予30 mg/kg甲基强的松龙、2.5 mg/kg环磷酰胺腹腔注射;维生素C1组、C2组在中毒组基础上给予两种浓度的维生素C(5,500 mg/kg),以生理盐水稀释成5 m L/kg,股静脉注射;对照组仅以生理盐水进行相应处理。于实验后36 h检测大鼠血浆中丙二醛、肝肾功能,并行动脉血气分析,采血后处死大鼠取肺组织行病理切片、湿干重比等检查;取肝组织行比色法检测谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX),Western印迹检测Bcl-2表达等。结果:维生素C1组中丙二醛低于中毒组,GSH-PX高于中毒组,肝肾功能较中毒组有明显改善(均P0.01);维生素C2组丙二醛高于中毒组,其肝肾功能较中毒组也呈恶化趋势(均P0.01)。中毒组中Bcl-2较对照组表达减低,维生素C1组中Bcl-2较中毒组表达增强,而维生素C2组中Bcl-2较中毒组表达减弱(均P0.01)。但维生素C1组、C2组在肺功能、湿干重比、病理学等与中毒组差异无统计学意义(均P0.05)。结论:低剂量维生素C可能通过抗氧化、抑制PQ导致的细胞凋亡等途径对PQ中毒大鼠的肝肾功能有部分的保护作用,而高剂量维生素C反而有促氧化作用;同时,维生素C未呈现出对PQ中毒时肺的保护作用。
[Abstract]:Objective: to investigate the efficacy of vitamin C in the treatment of paraquat (paraquat,PQ) poisoning in rats. Methods: 40 SpragueDawley (SD) rats were randomly divided into three groups: control group, intoxication group, vitamin C 1 group and C 2 group, with 10 rats in each group. The rat model of PQ poisoning SD was established by intragastric infusion of 150 mg/kg PQ,. In the poisoning group, 30 mg/kg methylprednisolone was injected intraperitoneally with 2.5 mg/kg cyclophosphamide. Two concentrations of vitamin C (5500 mg/kg) were given to vitamin C group and C2 group, diluted into 5 mL 路kg ~ (- 1) with saline and injected intravenously into femoral vein, while the control group was treated with normal saline only. Malondialdehyde (MDA) in plasma and function of liver and kidney were measured at 36 h after the experiment, and blood gas analysis was performed. Lung tissue was sacrificed after blood collection for pathological section and wet-dry weight ratio (WW / W). The expression of glutathione peroxidase (Bcl-2) in liver tissue was detected by glutathione peroxidase,GSH-PX), Western blot. Results: MDA in vitamin C1 group was lower than that in poisoning group, and GSH-PX was higher than that in poisoning group. The liver and kidney function of vitamin C1 group was significantly improved than that of poisoning group (all P0.01). The MDA of vitamin C _ 2 group was higher than that of poisoning group, and the liver and kidney function of vitamin C _ 2 group was worse than that of poisoning group (P 0.01). The expression of Bcl-2 in the poisoning group was lower than that in the control group, the expression of Bcl-2 in the vitamin C1 group was higher than that in the poisoning group, and the expression of Bcl-2 in the vitamin C2 group was lower than that in the poisoning group (P0.01). However, there was no significant difference in lung function, wet-dry weight ratio and pathology between vitamin C _ 1 and C _ 2 groups (P 0.05). Conclusion: low dose vitamin C may have partial protective effect on liver and kidney function of PQ poisoned rats by anti-oxidation and inhibiting apoptosis induced by PQ, while high dose vitamin C can promote oxidation. At the same time, vitamin C did not show a protective effect on the lung after PQ poisoning.
【作者单位】：重庆医科大学第二临床学院急救部;
【基金】：重庆市卫生局基金(2013-2-036)~~
【分类号】：R595.4

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