白桦脂醇拮抗地塞米松诱导成骨细胞内脂质积聚影响细胞凋亡的实验研究
发布时间：2019-03-23 17:34
【摘要】：目的观察白桦脂醇和地塞米松共同作用于小鼠MC3T3-E1细胞后,内源性脂质蓄积与细胞凋亡中的关系。方法以MC3T3-E1细胞为研究对象,采用白桦脂醇和地塞米松共同作用于MC3T3-E1细胞,测定细胞内甘油三酯含量;NileRed荧光染色观察脂质蓄积情况;流式细胞技术测定成骨细胞凋亡率;ELISA法检测Caspase-3酶活性;采用Spearman等级相关进行脂质含量与成骨细胞凋亡相关性分析。结果白桦脂醇和地塞米松共同作用于小鼠MC3T3-E1细胞后,0.5μmol/L地塞米松(B组)与1.0μmol/L地塞米松组(C组)较空白组(A组),细胞凋亡率、Caspase-3酶活性均明显增高(P0.05),细胞内甘油三酯含量明显增加。2.0μg/ml白桦脂醇作用细胞后,地塞米松所致的细胞凋亡率上升与Caspase-3酶活性升高受到明显抑制,同时细胞内的甘油三酯蓄积降低。Spearman等级相关分析表明地塞米松诱导后的成骨细胞内脂质含量与细胞凋亡率呈正相关,r=0.412,P0.05。结论白桦脂醇可显著减轻地塞米松诱导的成骨细胞内脂质蓄积,降低细胞凋亡率;提示地塞米松所致细胞凋亡至少部分与细胞内脂质蓄积有关。
[Abstract]:Aim to investigate the relationship between endogenous lipid accumulation and apoptosis in MC3T3-E1 cells treated with Bai Hua fatty alcohol and dexamethasone. Methods MC3T3-E1 cells were treated with Bai Hua fatty alcohol and dexamethasone to determine the intracellular triglyceride content, NileRed fluorescence staining was used to observe the accumulation of lipid. The apoptosis rate of osteoblasts was measured by flow cytometry, the activity of Caspase-3 enzyme was measured by ELISA method, and the correlation between lipid content and apoptosis of osteoblasts was analyzed by Spearman grade correlation. Results after the MC3T3-E1 cells were treated with Bai Hua fatty alcohol and dexamethasone, 0.5 渭 mol / L dexamethasone (group B) and 1.0 渭 mol / L dexamethasone group (group C) were more effective than those of control group (group A), and the apoptosis rate was higher than that of control group (group A). The activity of Caspase-3 enzyme was significantly increased (P0.05) and the content of triglyceride in the cells was significantly increased. After treated with 2.0 渭 g / ml Bai Hua lipid alcohol, the increase of apoptosis rate and the increase of Caspase-3 enzyme activity induced by dexamethasone were significantly inhibited. Spearman rank correlation analysis showed that the lipid content in osteoblasts induced by dexamethasone was positively correlated with the apoptosis rate (r = 0.412, P 0.05). Conclusion Bai Hua fatty alcohol can significantly reduce the lipid accumulation and apoptosis rate of osteoblasts induced by dexamethasone, suggesting that the apoptosis induced by dexamethasone is at least partly related to the accumulation of intracellular lipid.
【作者单位】： 广东医科大学附属医院骨科中心;
【基金】：广东省科技计划项目(2012B031800490;2011B031800172)
【分类号】：R580
，
本文编号：2446085
[Abstract]:Aim to investigate the relationship between endogenous lipid accumulation and apoptosis in MC3T3-E1 cells treated with Bai Hua fatty alcohol and dexamethasone. Methods MC3T3-E1 cells were treated with Bai Hua fatty alcohol and dexamethasone to determine the intracellular triglyceride content, NileRed fluorescence staining was used to observe the accumulation of lipid. The apoptosis rate of osteoblasts was measured by flow cytometry, the activity of Caspase-3 enzyme was measured by ELISA method, and the correlation between lipid content and apoptosis of osteoblasts was analyzed by Spearman grade correlation. Results after the MC3T3-E1 cells were treated with Bai Hua fatty alcohol and dexamethasone, 0.5 渭 mol / L dexamethasone (group B) and 1.0 渭 mol / L dexamethasone group (group C) were more effective than those of control group (group A), and the apoptosis rate was higher than that of control group (group A). The activity of Caspase-3 enzyme was significantly increased (P0.05) and the content of triglyceride in the cells was significantly increased. After treated with 2.0 渭 g / ml Bai Hua lipid alcohol, the increase of apoptosis rate and the increase of Caspase-3 enzyme activity induced by dexamethasone were significantly inhibited. Spearman rank correlation analysis showed that the lipid content in osteoblasts induced by dexamethasone was positively correlated with the apoptosis rate (r = 0.412, P 0.05). Conclusion Bai Hua fatty alcohol can significantly reduce the lipid accumulation and apoptosis rate of osteoblasts induced by dexamethasone, suggesting that the apoptosis induced by dexamethasone is at least partly related to the accumulation of intracellular lipid.
【作者单位】： 广东医科大学附属医院骨科中心;
【基金】：广东省科技计划项目(2012B031800490;2011B031800172)
【分类号】：R580
，
本文编号：2446085
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