NETs刺激下慢性支气管炎大鼠血清ANCA产生机制探讨

发布时间：2019-03-26 10:23

【摘要】：目的:探讨长期慢性感染刺激诱导机体产生抗中性粒细胞胞浆抗体(ANCA)的机制。方法:烟熏法联合脂多糖气管内注入,建立慢性支气管炎大鼠模型,用佛波脂(PMA)体外刺激大鼠中性粒细胞产生中性粒细胞胞外网络(NETs),以NETs免疫慢性支气管炎大鼠,比较慢性支气管炎NETs刺激组(CB/NETs)、慢性支气管炎组(CB)和正常组三组大鼠血清髓过氧化物酶-抗中性粒细胞胞浆抗体(MPO-ANCA)、血清瓜氨酸化的组蛋白H3(Cit H3)、血清IFN-α水平及血清肌酐、血清尿素氮、尿红细胞计数、24 h尿蛋白定量水平。结果:(1)CB/NETs组的血清MPO-ANCA水平明显高于CB组及正常组,(62.4975±19.637 64)ng/ml vs(17.380 6±4.886 49)ng/ml,(20.888 4±4.396 07)ng/ml(P0.01)。(2)CB/NETs组的血清Cit H3水平明显高于CB组及正常组,(32.597 4±20.291 5)ng/ml vs(8.856 8±2.366 92)ng/ml,(5.622 5±0.837 38)ng/ml,(P0.05,P0.01)。(3)CB/NETs组中有两只大鼠的血清IFN-α水平异常升高(3 735.7、3 428.4 pg/ml)。(4)三组大鼠的肾脏相关指标差异暂未呈现出统计学意义。(5)大鼠血清Cit H3水平与血清MPO-ANCA水平呈正相关(r=0.61,P0.05)。结论:慢性支气管炎长期、反复感染可导致ANCA水平升高,从形成高水平的ANCA到最终形成ANCA相关性小血管炎尚需要时间。
[Abstract]:Objective: to explore the mechanism of anti-neutrophil cytoplasmic antibody (ANCA) induced by chronic infection. Methods: the rat model of chronic bronchitis was established by fumigation combined with lipopolysaccharide injection. The rat neutrophil extracellular network (NETs),) was stimulated by phorbol ester (PMA) in vitro and NETs was used to immunize chronic bronchitis rats. The levels of myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA), citrullinated histone H3 (Cit H3) and myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA) in serum of chronic bronchitis group (CB/NETs), chronic bronchitis group (CB) and normal group were compared. The levels of serum IFN- 伪 and creatinine, serum urea nitrogen, urine red blood cell count and 24 h urinary protein were measured. Results: (1) the level of serum MPO-ANCA in CB/NETs group was significantly higher than that in CB group and normal group (62.4975 卤19.637 64) ng/ml vs (17.380 6 卤4.886 49) ng/ml,. The serum Cit H3 level in (20.888 4 卤4.396 07) ng/ml (P0.01). (2) CB/NETs group was significantly higher than that in CB group and normal group (32.597 4 卤20.291 5) ng/ml vs (8.856 8 卤2.366 92) ng/ml,. (5.622 5 卤0.837 38) ng/ml, (P 0.05, P0.01.). (- 3) the serum IFN- 伪 level of two rats in CB/NETs group increased abnormally (3735.7, 3 428.4 pg/ml). (4). There was no significant difference in renal related indexes among the three groups. (5) the level of serum Cit H3 was positively correlated with the level of serum MPO-ANCA (r = 0.61, P < 0.01). (P0.05). Conclusion: long-term chronic bronchitis, repeated infection can lead to an increase in the level of ANCA, from the formation of a high level of ANCA to the final formation of ANCA-associated small vasculitis will take time.
【作者单位】：青岛大学附属医院肾病科;
【基金】：国家自然科学基金(纵向:81770699) 山东省医药卫生科技发展计划项目(2014WS0176) 青岛市科技局成果转化计划科技惠民专项(纵向:15-9-2-90-nsh)资助
【分类号】：R593.2

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