延边朝鲜族ARL15和脂联素两个基因联合作用与2型糖尿病相关性研究
[Abstract]:Aim to investigate the relationship between the combination of three single nucleotide polymorphisms (SNPs) of ADP- ribonucleotide like factor 15 (ADP-ribosylation factor-like 15, ARL15) and adiponectin gene and type 2 diabetes mellitus,T2DM in Yanbian Korean nationality. Methods A case-control design was used to randomly select 144 patients with T2DM and 140 controls with normal glucose tolerance (NGT). Single base extension method was used to detect the genotypes of rs26770 (A? G) of ARL15 gene, rs1501299 (C) and rs2241766 (T? G) of adiponectin gene. The levels of ARL15 (PARL15), adiponectin (PA) and insulin (INS) in fasting plasma were measured by enzyme linked immunosorbent assay (enzyme linked immunosorbent assay, ELISA), and the physiological and biochemical indexes (weight, height, waist hip ratio, fasting blood glucose (FPG),) were also measured. Total cholesterol, (TC), triglyceride, (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol, low density lipoprotein cholesterol (LDL-C). Results 1.3allele frequencies and genotype frequencies of SNPs were in accordance with Hardy-Weinberg equilibrium law between NGT group and T2DM group (P0.05). However, no significant difference was found in allele frequency and genotype frequency between T2DM group and NGT group (P0.05). The combined genotype analysis (rs26770,rs1501299 and rs2241766) showed that the frequency of GG-AA-TT co-genotype in T2DM group (5.6%) was significantly higher than that in NGT group (0.0%). 3. The content of PA in T2 DM patients was significantly lower than that in NGT group, while the PARL15 content was significantly higher than that in NGT group. The results of association analysis between SNP genotypes and clinical indexes showed for the first time that the PA level of rs2241766-TT genotype of adiponectin gene in Yanbian Korean group was significantly lower than that of GG GT genotype, which was significantly lower than that of adiponectin gene rs2241766-TT genotype in Yanbian Korean nationality. The level of TG was significantly higher than that of GG GT genotype (P0.05), showing a dominant genetic model. The HDL-C of rs26770-GG genotype of ARL15 gene was lower than that of AA AG genotype (P0.05), and the BMI of rs26770-GG genotype of ARL15 gene was lower than that of AA AG genotype in T2DM group (P0.05), while PA was higher than that of AA AG (P0.05), and that of rs26770-GG genotype of ARL15 gene was lower than that of AA AG genotype (P0.05). Recessive inheritance pattern. 5. Compared with HapMap database, the allele frequencies of three SNPs alleles in Korean population in Yanbian, China, were significantly lower than those in European (CEU) (P0.01), and were significantly lower than those of (CHB), in Chinese Han nationality in Beijing (P0.01). There was no significant difference in (YRI) between (JPT) from Tokyo, Japan and Yoruba from Ibadan, Nigeria (P0.05). The allele frequency of rs1501299-A was lower than that of YRI (P0.05), and there was no significant difference with CHB,JPT and YRI (P0.05). The G frequency of rs2241766 allele was significantly higher than that of CEU and YRI (P0.01), but had no significant difference with CHB,JPT (P0.05). Conclusion the combined genotype GG-AA-TT of 1.ARL15 and adiponectin gene three SNPs (rs26770,rs1501299,rs2241766) may be a risk genotype combination of T2DM in Yanbian Korean nationality. Adiponectin gene rs2241766-G up-regulated the level of PA and down-regulated the level of TG; The frequency of SNPs (rs26770, rs1501299,rs2241766) allele of 4.ARL15 gene and adiponectin gene was significantly different between 4.ARL15 gene and adiponectin gene.
【学位授予单位】:延边大学
【学位级别】:硕士
【学位授予年份】:2015
【分类号】:R587.1
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