2型糖尿病患者IL-18、脂联素与认知功能的相关性研究

发布时间：2019-03-31 19:27

【摘要】：目的本实验通过检测2型糖尿病(Type 2 diabetes mellitus,T2DM)患者白介素18(Interleukin,IL-18)和脂联素(Adiponectin,APN)水平,探讨T2DM患者血清IL-18和APN水平与认知功能的关系,及其各自的影响因素。方法1研究对象病例组选自2013年3月至2014年3月于唐山市工人医院内分泌二科住院的T2DM患者65例,男36例,女29例。另外,正常对照组于唐山工人医院院体检中心选取,共50例,男27例,女23例,两组年龄匹配。病例组中,根据IL-18水平是否大于其均数分为IL-18-H、IL-18-L两组,同理,脂联素分为APN-H、APN-L两组。2研究方法采集受试者肘静脉血,于抽血当天测定受试者FPG、Hb A1c、TC、TG、HDL、LDL等临床指标,并进行RBANS量表测试。制定一般情况调查表,收集受试者一般资料包括:性别、年龄、教育程度、病程、体重指数(body mass index,BMI)。采用酶联免疫吸附法(Enzyme linked immunosorbent assay,ELLSA)测定血清IL-18、APN水平,上述资料应用SPSS17.0进行统计分析结果1病例组TG、FPG、Hb A1c、IL-18水平高于对照组,HDL、APN水平低于对照组,差异有统计学意义(P0.05),两组其余指标差异无统计学意义(P0.05)。2病例组即时记忆分数及标准分低于对照组,差异有统计学意义(P0.05),但两组视觉广度分数、言语功能分数、注意力分数、延迟记忆分数差异无统计学意义(P0.05)。3 IL-18-H组即时记忆分数、言语功能分数、注意力分数、延迟记忆分数及标准分低于IL-18-L组,差异有统计学意义(P0.05),视觉广度分数差异无统计学意义(P0.05);APN-H组与APN-L组比较,APN-H组即时记忆分数、注意力分数、标准分高于APN-L组,差异有统计学意义(P0.05),视觉广度分数、言语功能分数、延迟记忆分数差异无统计学意义(P0.05)。4 IL-18水平与年龄、FPG、Hb A1c呈正相关,与APN水平、即时记忆分数、言语功能分数、注意力分数、延迟记忆分数、标准分呈负相关(P0.05);APN水平与Hb A1C、IL-18水平呈负相关,与即时记忆分数、注意力分数、标准分呈正相关(P0.05)。5以IL-18为应变量,APN、性别、年龄、教育程度、BMI、TC、TG、HDL、LDL、FPG、Hb A1c、病程为自变量进行多元逐步回归分析,进入回归方程的有:年龄、Hb A1c、APN;以APN为应变量,IL-18、性别、年龄、教育程度、BMI、TC、TG、HDL、LDL、FPG、Hb A1c、病程为自变量进行多元逐步回归分析,进入回归方程的有:IL-18、TC;分别以即时记忆分数、视觉广度分数、言语功能分数、注意力分数、延迟记忆分数、标准分为应变量,IL-18、APN、性别、年龄、教育程度、BMI、TC、TG、HDL、LDL、FPG、Hb A1c、病程为自变量进行多元逐步回归分析,进入方程的分别有:教育程度、IL-18;病程;年龄、教育程度;年龄、教育程度、IL-18、APN;IL-18;年龄、教育程度、IL-18、APN。结论1 T2DM患者存在炎症状态,其血清IL-18水平显著升高、APN水平显著降低,并伴随认知功能损伤。2随着IL-18水平的升高和APN水平的降低T2DM患者的认知功能显著下降。3 APN可能通过抗炎机制改善认知功能。
[Abstract]:Objective to investigate the relationship between serum levels of interleukin-18 (Interleukin,IL-18) and adiponectin (Adiponectin,APN) in patients with type 2 diabetes mellitus (Type-2 diabetes mellitus,T2DM) and cognitive function by measuring serum levels of IL-18 and APN in patients with type 2 diabetes mellitus (T2DM). And their respective influencing factors. Methods (1) 65 patients (36 males and 29 females) with T2DM were selected from March 2013 to March 2014 in the second Department of Endocrinology of Tangshan Workers' Hospital. In addition, the normal control group was selected from the physical examination center of Tangshan Workers Hospital. There were 50 cases, 27 males and 23 females. The age of the two groups was matched. In the case group, the patients were divided into two groups according to whether or not IL-18 level was greater than its mean. Similarly, adiponectin was divided into two groups: APN-H,APN-L group and control group. (2) Blood samples were collected from the elbow vein of the subjects. The clinical indexes such as FPG,Hb A1C, TC, TG, HDL, LDL were measured on the day of blood sampling, and the RBANS scale was used. A general questionnaire was developed to collect subjects' general data including: sex, age, education level, course of disease, body mass index (body mass index,BMI). The level of serum IL-18,APN was measured by enzyme-linked immunosorbent assay (Enzyme linked immunosorbent assay,ELLSA). The above data were analyzed by SPSS17.0. The results showed that the levels of TG,FPG,Hb A1c and IL-18 in the case group were higher than those in the control group, HDL,. APN level was lower than the control group, the difference was statistically significant (P0.05), there was no significant difference in other indicators between the two groups (P0.05). 2 the immediate memory score and standard score in the case group were lower than those in the control group, the difference was statistically significant (P0.05). But there was no significant difference in visual span score, speech function score, attention score and delayed memory score between the two groups (P0.05). 3 in IL-18-H group, immediate memory score, speech function score and attention score were not significantly different (P0.05). Delayed memory scores and standard scores were lower than those in IL-18-L group (P0.05), but visual breadth scores had no significant difference (P0.05). Compared with APN-L group, the instant memory score, attention score and standard score in APN-H group were higher than those in APN-L group (P0.05), visual breadth score, speech function score, and so on. There was no significant difference in delayed memory score (P0.05). 4 IL-18 level was positively correlated with age, FPG,Hb A1c, APN level, instant memory score, speech function score, attention score and delayed memory score. The standard score was negatively correlated (P0.05); APN level was negatively correlated with Hb A1C, IL-18 level, and positively correlated with instant memory score, attention score and standard score (P0.05). 5 IL-18 was used as dependent variable, APN, sex, age, education level, BMI,TC,TG,HDL,LDL,. FPG,Hb A1c, course of disease as independent variable for multiple stepwise regression analysis, entered the regression equation are: age, Hb A1c, APN; Taking APN as dependent variable, IL-18, sex, age, education level, BMI,TC,TG,HDL,LDL,FPG,Hb A1c and course of disease as independent variables, the multiple stepwise regression analysis was carried out. The regression equation was IL-18,TC;. According to immediate memory score, visual span score, speech function score, attention score, delayed memory score, IL-18,APN, gender, age, education level, BMI,TC,TG,HDL,LDL,FPG,Hb A1c, the standard was classified as dependent variable, visual span score, speech function score, attention score and delayed memory score. The course of disease was independent variable and the multiple stepwise regression analysis was carried out. The entry equations were: education level, IL-18;. Course of disease; age, education level, IL-18,APN;IL-18; age, education level, IL-18,APN. Conclusion (1) there is inflammatory state in T2DM patients. The level of serum IL-18 is significantly higher and the level of APN is significantly lower in patients with SARS. 2 with the increase of IL-18 level and the decrease of APN level, the cognitive function of T2DM patients decreased significantly. 3 APN may improve cognitive function through anti-inflammatory mechanism.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.1

【共引文献】