钙螯合胶原多肽与雌激素对去卵巢大鼠骨质改善作用的比较

发布时间：2019-04-08 18:16

【摘要】：目的将钙螯合胶原多肽(CPCC)的骨质改善效果与雌激素进行比较,为安全的骨质疏松(osteoporosis,OP)防治药物的研发奠定基础。方法切除大鼠双侧卵巢,设置切卵巢组(OVX)、假手术组(sham)、17β-雌二醇(OVX+E_2)注射组与CPCC灌胃组(OVX+CPCC),9周后比较各组的骨骼指标和血液生化指标。结果 OVX组股骨密度显著低于sham组(P0.01),说明OP发生。和激素一样,CPCC可有效抑制所测指标的异常变化,并使其维持在sham组水平(P0.05)。然而在抑制体重增加方面,E_2组在第8、9周体重显著低于sham组(P0.01);在阻止骨矿和骨有机质流失方面,E_2组的Mg、Ca水平显著低于CPCC的中、高剂量组,Cu水平与sham组无差异,而CPCC(中、低剂量)则显著高于sham组。E_2组的Mn、Zn、及羟脯氨酸水平显著低于sham组,而CPCC则可维持在sham组水平。对血液中BGP和Str ACP水平升高的抑制作用,E_2组与OVX组相比差异无显著性,CPCC组则可显著低于OVX组。抑制血钙水平下降时,E_2组与OVX组相比差异无显著性,而CPCC组显著高于OVX组。结论在改善去卵巢大鼠骨质方面,钙螯合胶原多肽比雌激素更为有效。
[Abstract]:Aim to compare the bone improvement effect of calcium chelated collagen peptide (CPCC) with estrogen in order to lay a foundation for the research and development of safe preventive and therapeutic drugs for osteoporosis (osteoporosis,OP). Methods the bilateral ovaries of the rats were resected and the bone and blood biochemical indexes of (sham), 17 尾-estradiol (sham),-17 尾-estradiol) (OVX-17 尾-estradiol) injection group and CPCC perfusion group were compared 9 weeks after ovariectomy with (OVX), sham operation group and CPCC instillation group. Results the femoral density in OVX group was significantly lower than that in sham group (P0.01), indicating the occurrence of OP. Like hormone, CPCC can effectively inhibit the abnormal changes of the measured indexes and keep them at the level of sham group (P0.05). However, on the aspect of inhibiting weight gain, the body weight of E-2 group was significantly lower than that of sham group at the 8th and 9th week (P0.01). In preventing bone mineral and bone organic matter loss, the level of Mg,Ca in E 2 group was significantly lower than that in CPCC group, the level of Cu in high dose group was similar to that in sham group, but the level of CPCC (middle and low dose) was significantly higher than that in sham group. The Mn,Zn, in E 2 group was significantly higher than that in E 2 group. The levels of hydroxyproline and hydroxyproline were significantly lower in the sham group than in the sham group, while the CPCC level was maintained in the sham group. There was no significant difference in the levels of BGP and Str ACP between the two groups compared with the OVX group, but that in the CPCC group was significantly lower than that in the OVX group. When the serum calcium level was inhibited, there was no significant difference between E _ 2O _ 2 group and OVX group, but that in CPCC group was significantly higher than that in OVX group. Conclusion calcium chelate collagen peptide is more effective than estrogen in improving the bone of ovariectomized rats.
【作者单位】：山西农业大学动物科技学院;山西省农业科技信息中心;
【基金】：国家自然科学基金(编号:31201347) 山西省回国留学人员项目(编号:2014-042)
【分类号】：R580

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